Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells

1994 ◽  
Vol 159 (1) ◽  
pp. 101-113 ◽  
Author(s):  
F. Roux ◽  
O. Durieu-Trautmann ◽  
N. Chaverot ◽  
M. Claire ◽  
P. Mailly ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Endothelial Cells ◽  
Rat Brain ◽  
Gamma Glutamyl Transpeptidase ◽  
Phosphatase Activities ◽  
Gamma Glutamyl ◽  
Microvessel Endothelial Cells ◽  
Brain Microvessel ◽  
Glutamyl Transpeptidase

Modulation of endothelial GSH concentrations: effect of exogenous GSH and GSH monoethyl ester

1989 ◽  
Vol 66 (3) ◽  
pp. 1029-1034 ◽  
Author(s):  
M. F. Tsan ◽  
J. E. White ◽  
C. L. Rosano
Keyword(s):  
Endothelial Cells ◽  
Trace Amount ◽  
Potent Inhibitor ◽  
Buthionine Sulfoximine ◽  
Diethyl Ester ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Intracellular Concentrations ◽  
Glutamyl Transpeptidase

We studied the effects of exogenous glutathione (GSH) and GSH monoethyl ester (GSH-MEE) on the enhancement of endothelial GSH concentrations. The preparation of GSH-MEE used contained 91% GSH-MEE, approximately 9% GSH diethyl ester (GSH-DEE) and a trace amount of GSH. Both GSH and GSH-MEE markedly stimulated the intracellular concentrations of GSH in endothelial cells. GSH-MEE was more potent than GSH. The enhancement of endothelial GSH concentration by exogenous GSH was completely inhibited by buthionine sulfoximine (BSO), a potent inhibitor of gamma-glutamylcysteine synthase, or acivicin (AT-125), an inhibitor of gamma-glutamyl transpeptidase, suggesting that it was due to the extracellular breakdown and subsequent intracellular resynthesis of GSH. In contrast, the effect of GSH-MEE was largely resistant to BSO and acivicin, suggesting that it was primarily due to transport of GSH-MEE followed by intracellular hydrolysis. The GSH-MEE preparation, which contained 9% GSH-DEE, at concentrations of 2 mM or higher caused vacuolization of endothelial cells. The enhancement of GSH concentrations by exogenous GSH, but not by GSH-MEE, protected endothelial cells against H2O2-induced injury.

scholarly journals Assessment of liver enzymes in saliva and serum of Iraqi patients with chronic periodontitis disease

2020 ◽  
Vol 14 (1) ◽  
pp. 20-24
Author(s):  
Hussein SH. Ridha ◽  
Zahraa H.M. Kadri
Keyword(s):  
Alkaline Phosphatase ◽  
Chronic Periodontitis ◽  
Alanine Aminotransferase ◽  
Liver Enzymes ◽  
Serum Levels ◽  
Aspartate Transaminase ◽  
Gamma Glutamyl Transpeptidase ◽  
Serum Samples ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

Objective: The present study aimed to assess of four liver enzymes, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT). Material and Methods: Based on periodontal clinical parameters, sixty four patients with chronic periodontitis (CP) and twenty four controls were enrolled in the study. Saliva and serum samples were collected and Automated Chemistry Analyzer AU 480 was employed to assess levels of enzymes. Results: Compared to healthy controls, the levels of the four enzymes were significant increased in serum of patients, especially in the severe group while in the saliva a significant increase observed only in the level of AST. Moreover, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT) the levels of these enzymes in serum were significantly higher than those in saliva. Conclusion: ALT, AST, ALP and GGT serum levels are suggested to be important indicators for disease progression as well as predict the liver health.  

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