Platelet-derived growth factor-BB-induced hypertrophy of peritubular smooth muscle cells is mediated by activation of p38 MAP-kinase and of Rho-kinase

2006 ◽  
Vol 207 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Francesca Romano ◽  
Claudia Chiarenza ◽  
Fioretta Palombi ◽  
Antonio Filippini ◽  
Fabrizio Padula ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Map Kinase ◽  
Rho Kinase ◽  
Muscle Cells ◽  
P38 Map Kinase ◽  
Platelet Derived Growth Factor

scholarly journals Contribution of Rho A and Rho Kinase to Platelet-Derived Growth Factor-BB-Induced Proliferation of Vascular Smooth Muscle Cells.

2003 ◽  
Vol 10 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Masumi Kamiyama ◽  
Kazunori Utsunomiya ◽  
Kanta Taniguchi ◽  
Tamotsu Yokota ◽  
Hideaki Kurata ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Platelet Derived Growth Factor ◽  
Rho A

scholarly journals Treatment of vascular smooth muscle cells with antisense phosphorothioate oligodeoxynucleotides directed against p42 and p44 mitogen-activated protein kinases abolishes DNA synthesis in response to platelet-derived growth factor

1996 ◽  
Vol 320 (1) ◽  
pp. 123-127 ◽  
Author(s):  
Caspar J. M. ROBINSON ◽  
Pamela H SCOTT ◽  
Andrew B ALLAN ◽  
Thomas JESS ◽  
Gwyn W. GOULD ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Map Kinase ◽  
Kinase Activity ◽  
Thymidine Incorporation ◽  
Muscle Cells ◽  
Platelet Derived Growth Factor ◽  
Mitogen Activated Protein

We have investigated the requirement for mitogen-activated protein (MAP) kinase in the stimulation of DNA synthesis by platelet-derived growth factor (PDGF) in rat aortic smooth muscle cells using a phosphorothioate-modified oligodeoxynucleotide (ODN) to deplete MAP kinase. Treatment for 72 h with MAP kinase antisense ODN directed against both the p42 and p44 isoforms of MAP kinase abolished the expression of MAP kinase and reduced agonist-stimulated MAP kinase activity by approx. 95%. The scrambled control ODN was without effect, but the sense control ODN slightly enhanced the expression of both isoforms. Abolition of MAP kinase activity by antisense ODN treatment prevented angiotensin II- and PDGF-stimulated activation of p90 ribosomal S6 kinase activity, but did not affect activation of MAP kinase kinase. In addition, antisense ODN pretreatment reduced PDGF-stimulated [3H]thymidine incorporation to < 5% of control, and decreased basal incorporation by approx. 90%. In contrast, basal [3H]thymidine incorporation was enhanced approx. 60% by control sense ODN treatment. These results indicate an obligatory role for MAP kinase in the activation of a number of early events in mitogenesis, including DNA synthesis, in vascular smooth muscle cells.

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