In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration

Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here, we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa, or vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.

Wavelength of a Turing-type mechanism regulates the morphogenesis of meshwork patterns

The meshwork pattern is a significant pattern in the development of biological tissues and organs. It is necessary to explore the mathematical mechanism of meshwork pattern formation. In this paper, we found that the meshwork pattern is formed by four kinds of stalk behaviours: stalk extension, tip bifurcation, side branching and tip fusion. The Turing-type pattern underlying the meshwork pattern is a Turing spot pattern, which indicates that the Turing instability of the spot pattern promotes activator peak formation and then guides the formation of meshwork patterns. Then, we found that the Turing wavelength decreased in turn from tip bifurcation to side branching to tip fusion via statistical evaluation. Through the functional relationship between the Turing wavelength and model parameters ($$\upvarepsilon ,{ \rho }_{A}$$ ε , ρ A and $${\rho }_{H}$$ ρ H ), we found that parameters $$\upvarepsilon $$ ε and $${\rho }_{H}$$ ρ H had monotonic effects on the Turing wavelength and that parameter $${\rho }_{A}$$ ρ A had nonmonotonic effects. Furthermore, we performed simulations of local meshwork pattern formation under variable model parameter values. The simulation results verified the corresponding relationship between the Turing wavelength and stalk behaviours and the functional relationship between the Turing wavelength and model parameters. The simulation results showed that the Turing wavelength regulated the meshwork pattern and that the small Turing wavelength facilitated dense meshwork pattern formation. Our work provides novel insight into and understanding of the formation of meshwork patterns. We believe that studies associated with network morphogenesis can benefit from our work.

In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration

Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying unique proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa or Vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.

Psychometric Properties and Urdu Translation of Wong and Law Emotional Intelligence Scale (WLEIS)

<p>The purpose of this study is to translate “Wong and Law Emotional Intelligence Scale” (WLEIS; Wong & Law, 2002) into Urdu and also to determine the psychometric properties of this scale. The final (Urdu) version of the scale is checked on a sample of 315 managers of the top four private commercial banks of Pakistan after the translation process. The validity and reliability of the WLEIS were evaluated by analyzing the “Cronbach alpha”, composite reliability, rho-A, and average variance extracted. The following hypotheses were formulated after detailed literature review (a) the Urdu version of WLEIS scores were positively correlated to three dimensions of job performance (JP) (b) Work engagement (WE) mediates the association between Urdu translated dimensions of WLEIS and dimensions of JP. Reliability of the four-dimensional Urdu variant of WLEIS estimated by Cronbach's Alpha which is 0.67, 0.74, 0.77 and 0.75; rho A is 0.69, 0.76, 0.79 and 0.76 and composite reliability is .0.80, 0.84, 0.85 and 0.84 and AVE is 0.51, 0.56, 0.59 and 0.57 respectively. In addition, WLEIS-U dimensions have a statistically substantial positive association with three dimensions of JP and WE is proved to be mediator between WLEIS-U dimensions and dimensions of job performance. It has been found that the Urdu version of WLEIS in Pakistan is reliable.</p>

Psychometric Properties and Urdu Translation of Wong and Law Emotional Intelligence Scale (WLEIS)

<p>The purpose of this study is to translate “Wong and Law Emotional Intelligence Scale” (WLEIS; Wong & Law, 2002) into Urdu and also to determine the psychometric properties of this scale. The final (Urdu) version of the scale is checked on a sample of 315 managers of the top four private commercial banks of Pakistan after the translation process. The validity and reliability of the WLEIS were evaluated by analyzing the “Cronbach alpha”, composite reliability, rho-A, and average variance extracted. The following hypotheses were formulated after detailed literature review (a) the Urdu version of WLEIS scores were positively correlated to three dimensions of job performance (JP) (b) Work engagement (WE) mediates the association between Urdu translated dimensions of WLEIS and dimensions of JP. Reliability of the four-dimensional Urdu variant of WLEIS estimated by Cronbach's Alpha which is 0.67, 0.74, 0.77 and 0.75; rho A is 0.69, 0.76, 0.79 and 0.76 and composite reliability is .0.80, 0.84, 0.85 and 0.84 and AVE is 0.51, 0.56, 0.59 and 0.57 respectively. In addition, WLEIS-U dimensions have a statistically substantial positive association with three dimensions of JP and WE is proved to be mediator between WLEIS-U dimensions and dimensions of job performance. It has been found that the Urdu version of WLEIS in Pakistan is reliable.</p>

Searching optimum equations of state of neutron star matter in strong magnetic fields with rotation

Masses and radii of neutron stars are obtained in the presence of strong magnetic fields together with rotation. Mass-radius relations are calculated using 11 equations of state (EoSs: GM1, TM1-a, TM1-b, TM2$\omega\rho$-a, TM2$\omega\rho$-b, NL3-a, NL3-b, NL3$\omega\rho$-a, NL3$\omega\rho$-b, DDME2-a and DDME2-b) in relativistic mean field (RMF) theory. Obtained masses are over and around twice the solar mass ($M_\odot$) for all EoSs in the presence of strong magnetic fields of $3 \times 10^{18}$ G at the center. For NL3$\omega\rho$-a and NL3$\omega\rho$-b EoSs, masses are more than $M=2.17\,M_\odot$(observed maximum mass: $2.14\,M_\odot$) even without magnetic fields. Rotational effects are found to be insignificant in any case, at least up to the Kepler frequency. Suitable EoSs are also selected concerning the constraint on the radius of a neutron star.

Overexpression of microRNA-136-3p Alleviates Myocardial Injury in Coronary Artery Disease via the Rho A/ROCK Signaling Pathway

Objective: Coronary artery disease (CAD) is a cardiovascular disease that poses a fatal threat to human health, and the identification of potential biomarkers may help to delineate its pathophysiological mechanisms. Accumulating evidence has implicated microRNAs (miRNAs) in the pathogenesis and development of cardiovascular diseases. The present study aims to identify the expression of miRNA-136-3p (miR-136-3p) in CAD and further investigate its functional relevance in myocardial injury both in vitro and in vivo. Methods: Initially, CAD models were induced in rats by high-fat diet and intraperitoneal injection of pituitrin. Next, the effect of overexpressed miR-136-3p on cardiac function and pathological damage in myocardial tissue, cardiomyocyte apoptosis, oxidative stress and inflammatory response were assessed in CAD rats. Rat cardiac microvascular endothelial cells (CMECs) were isolated and cultured by the tissue explant method, and the CMEC injury model was induced by homocysteine (HCY). The function of miR-136-3p in vitro was further evaluated. Results: miR-136-3p was poorly expressed in the myocardial tissue of CAD rats and CMEC injury models. In vivo assays indicated that overexpressed miR-136-3p could improve cardiac function and alleviate pathological damage in myocardial tissue, accompanied by reduced oxidative stress and inflammatory response. Moreover,in vitro assays suggested that overexpression of miR-136-3p enhanced proliferation and migration while inhibiting apoptosis of HCY-stressed CMECs. Notably, we revealed that EIF5A2 was a target gene of miR-136-3p, and miR-136-3p inhibited EIF5A2 expression and activation of the Rho A/ROCK signaling pathway. Conclusion: In conclusion, the overexpression of miR-136-3p could potentially impede myocardial injury in vitro and in vivo in CAD through the blockade of the Rho A/ROCK signaling pathway, highlighting a potential miR-136-3p functional relevance in the treatment of CAD.