TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells

2012 ◽  
Vol 227 (11) ◽  
pp. 3593-3602 ◽  
Author(s):  
Daniel Vittet ◽  
Galina Merdzhanova ◽  
Marie-Hélène Prandini ◽  
Jean-Jacques Feige ◽  
Sabine Bailly
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lymphatic Endothelial Cell ◽  
Mouse Embryonic Stem Cells ◽  
Endothelial Cell Differentiation

An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells

2011 ◽  
Vol 32 (22) ◽  
pp. 3133-3137 ◽  
Author(s):  
Jong Min Lee ◽  
Ji-eun Kim ◽  
Edward Kang ◽  
Sang-Hoon Lee ◽  
Bong Geun Chung
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Endothelial Cell Differentiation

Leptin enhances endothelial cell differentiation and angiogenesis in murine embryonic stem cells

2015 ◽  
Vol 97 ◽  
pp. 65-74 ◽  
Author(s):  
Silvia Kurtovic ◽  
Tina T. Ng ◽  
Ankur Gupta ◽  
Vaithilingaraja Arumugaswami ◽  
Kira L. Chaiboonma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Endothelial Cell Differentiation

scholarly journals Post-meiotic male germ cell differentiation of mouse embryonic stem cells by exposure to conditioned medium

2019 ◽  
Vol 112 (3) ◽  
pp. e101-e102
Author(s):  
Aysha Trout ◽  
Philip Xie ◽  
Alessandra Parrella ◽  
Zev Rosenwaks ◽  
Gianpiero D. Palermo
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Conditioned Medium ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Male Germ Cell ◽  
Germ Cell Differentiation

scholarly journals Lin28 Inhibits the Differentiation from Mouse Embryonic Stem Cells to Glial Lineage Cells through Upregulation of Yap1

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Juan Luo ◽  
Hailin Zou ◽  
Liang Deng ◽  
Xiang Sun ◽  
Ping Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Glial Cell ◽  
Rna Binding ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Neuronal Marker ◽  
Glial Lineage

The RNA-binding protein Lin28 regulates neurogliogenesis in mammals, independently of the let-7 microRNA. However, the detailed regulatory mechanism remains obscured. Here, we established Lin28a or Lin28b overexpression mouse embryonic stem cells (ESCs) and found that these cells expressed similar levels of the core pluripotent factors, such as Oct4 and Sox2, and increased Yap1 but decreased lineage-specific markers compared to the control ESCs. Further differentiation of these ESCs to neuronal and glial lineage cells revealed that Lin28a/b overexpression did not affect the expression of neuronal marker βIII-tubulin, but dramatically inhibited the glial lineage markers, such as Gfap and Mbp. Interestingly, overexpression of Yap1 in mouse ESCs phenocopied Lin28a/b overexpression ESCs by showing defect in glial cell differentiation. Inhibition of Yap1/Tead-mediated transcription with verteporfin partially rescued the differentiation defect of Lin28a/b overexpression ESCs. Mechanistically, we demonstrated that Lin28 can directly bind to Yap1 mRNA, and the induction of Yap1 by Lin28a in mESCs is independent of Let7. Taken together, our results unravel a novel Lin28-Yap1 regulatory axis during mESC to glial lineage cell differentiation, which may shed light on glial cell generation in vitro.

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