scholarly journals Temporal changes in Hox gene expression accompany endothelial cell differentiation of embryonic stem cells

2011 ◽  
Vol 5 (2) ◽  
pp. 133-141 ◽  
Author(s):  
S. Bahram Bahrami ◽  
Mandana Veiseh ◽  
Ashley A. Dunn ◽  
Nancy J. Boudreau
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Temporal Changes ◽  
Hox Gene ◽  
Endothelial Cell Differentiation

An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells

2011 ◽  
Vol 32 (22) ◽  
pp. 3133-3137 ◽  
Author(s):  
Jong Min Lee ◽  
Ji-eun Kim ◽  
Edward Kang ◽  
Sang-Hoon Lee ◽  
Bong Geun Chung
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Endothelial Cell Differentiation

TGFβ1 inhibits lymphatic endothelial cell differentiation from mouse embryonic stem cells

2012 ◽  
Vol 227 (11) ◽  
pp. 3593-3602 ◽  
Author(s):  
Daniel Vittet ◽  
Galina Merdzhanova ◽  
Marie-Hélène Prandini ◽  
Jean-Jacques Feige ◽  
Sabine Bailly
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lymphatic Endothelial Cell ◽  
Mouse Embryonic Stem Cells ◽  
Endothelial Cell Differentiation

Leptin enhances endothelial cell differentiation and angiogenesis in murine embryonic stem cells

2015 ◽  
Vol 97 ◽  
pp. 65-74 ◽  
Author(s):  
Silvia Kurtovic ◽  
Tina T. Ng ◽  
Ankur Gupta ◽  
Vaithilingaraja Arumugaswami ◽  
Kira L. Chaiboonma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Endothelial Cell Differentiation

HOX gene analysis of endothelial cell differentiation in human bone marrow-derived mesenchymal stem cells

2007 ◽  
Vol 36 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Namhyun Chung ◽  
Bo Keun Jee ◽  
Song Wha Chae ◽  
Yang-Whan Jeon ◽  
Kweon Haeng Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Endothelial Cell ◽  
Cell Differentiation ◽  
Human Bone ◽  
Gene Analysis ◽  
Human Bone Marrow ◽  
Hox Gene ◽  
Endothelial Cell Differentiation

BMP4 Favors Hematopoietic Differentiation from Murine Embryonic Stem Cells and Acts by Activation of the cdx-hox Pathway.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3613-3613
Author(s):  
Claudia Lengerke ◽  
Yuan Wang ◽  
Frank Yates ◽  
Leila Maouche-Chretien ◽  
George Q. Daley
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hox Genes ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Hematopoietic Stem ◽  
Hox Gene ◽  
Murine Embryonic Stem Cells

Abstract Cdx4 and cdx1, members of the caudal family of homeodomain-containing transcriptional regulators, are important for specifying the hematopoietic fate of mesoderm in the zebrafish. We have shown that the cdx4 gene plays a role in enhancing hematopoietic fate during in vitro differentiation of murine ESCs (Davidson et al., Nature 2003). Cdx4 induces hox genes, and genetic modification of mESCs with a combination of cdx4 and hoxb4 promotes long-term engraftment of ESC-derived HSCs in lethally irradiated primary and secondary mice (Wang et al, submitted). While cdx1 is known to be a direct target of signaling by the embryonic morphogens fgf, wnt3a, and retinoids, morphogens acting upstream of cdx4 have not yet been defined. Our goal is to determine optimal morphogen conditions for hematopoietic commitment from murine embryonic stem cells by evaluating activation of the cdx-hox pathway. We have developed quantitative RT-PCR assays for the cdx genes (cdx4, cdx1 and cdx2) and multiple hox genes as well as markers specific to hematopoietic stem cells and lineages. We have used these assays, together with a reporter line engineered to express GFP from the brachury locus (Fehling et al., Development 2003), to characterize the conditions for mesodermal induction and hematopoietic fate specification following addition of morphogens to differentiating cultures of ES cells under serum-free conditions. Among all morphogens tested (BMP4, activin, nodal, wnt3a, wnt5a, sonic hedgehog, indian hedgehog, retinoic acid), only BMP4 has been found to strongly induce CDX4 gene expression within the developing embryoid bodies, while addition of the BMP4 inhibitor noggin to serum suppressed CDX4 expression. Addition of BMP4 significantly increases the number of emerging CD41+ and CD45+ cells, the precursors of definitive hematopoietic stem cells. We are currently analyzing the functional changes following BMP4 exposure, and correlating hematopoietic maturation with changes in the Hox gene expression pattern. Analysis of the cdx-hox gene pathway provides a means of otpimizing induction of hematopoietic fate by application of embryonic morphogens.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

scholarly journals The Trends in Global Gene Expression in Mouse Embryonic Stem Cells During Spaceflight

2019 ◽  
Vol 10 ◽  
Author(s):  
Lili An ◽  
Yanming Li ◽  
Yingjun Fan ◽  
Ning He ◽  
Fanlei Ran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Mouse Embryonic Stem Cells
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