Attached culture of Gibberella fujikuroi for biocomposite sorbent production and ciprofloxacin sequestration applications

Author(s):  
Tamer Akar ◽  
Fatih Sayin ◽  
Sema Celik ◽  
Sibel Tunali Akar
2008 ◽  
Vol 58 (1) ◽  
pp. 83-87 ◽  
Author(s):  
O. Modin ◽  
K. Fukushi ◽  
F. Nakajima ◽  
K. Yamamoto

Methane would potentially be an inexpensive, widely available electron donor for denitrification of wastewaters poor in organics. Currently, no methanotrophic microbe is known to denitrify. However, aerobic methane oxidation coupled to denitrification (AME-D) has been observed in several laboratory studies. In the AME-D process, aerobic methanotrophs oxidise methane and release organic metabolites and lysis products, which are used by coexisting denitrifiers as electron donors for denitrification. Due to the presence of oxygen, the denitrification efficiency in terms of methane-to-nitrate consumption is usually low. To improve this efficiency the use of a membrane biofilm reactor was investigated. The denitrification efficiency of an AME-D culture in (1) a suspended growth reactor, and (2) a membrane biofilm reactor was studied. The methane-to-nitrate consumption ratio for the suspended culture was 8.7. For the membrane-attached culture the ratio was 2.2. The results clearly indicated that the membrane-attached biofilm was superior to the suspended culture in terms of denitrification efficiency. This study showed that for practical application of the AME-D process, focus should be placed on development of a biofilm reactor.


1997 ◽  
Vol 25 (3) ◽  
pp. 557-560 ◽  
Author(s):  
Ulrike F. Schlacht ◽  
Evelyn M. Möller ◽  
Hartwig G. Geiger

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 175-189 ◽  
Author(s):  
Jin-rong Xu ◽  
John F Leslie

Abstract We constructed a recombination-based map of the fungal plant pathogen Gibberella fujikuroi mating population A (asexual stage Fusarium moniliforme). The map is based on the segregation of 142 restriction fragment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+ / matA−), female sterility (ste1), spore-killer (Sk), and a gene governing the production of the mycotoxin fumonisin B1 (fum1) among 121 random ascospore progeny from a single cross. We identified 12 linkage groups corresponding to the 12 chromosome-sized DNAs previously observed in contour-clamped homogeneous electric field (CHEF) gels. Linkage groups and chromosomes were correlated via Southern blots between appropriate RFLP markers and the CHEF gels. Eleven of the 12 chromosomes are meiotically stable, but the 12th (and smallest) is subject to deletions in 3% (4/121) of the progeny. Positive chiasma interference occurred on five of the 12 chromosomes, and nine of the 12 chromosomes averaged more than one crossover per chromosome. The average kb/cM ratio in this cross is ~32.


2011 ◽  
Vol 77 (9) ◽  
pp. 3023-3034 ◽  
Author(s):  
Ya-Jie Tang ◽  
Wei Zhao ◽  
Hong-Mei Li

ABSTRACTAccording to the structure of podophyllotoxin and its structure-function relationship, a novel tandem biotransformation process was developed for the directional modification of the podophyllotoxin structure to directionally synthesize a novel compound, 4-(2,3,5,6-tetramethylpyrazine-1)-4′-demethylepipodophyllotoxin (4-TMP-DMEP). In this novel tandem biotransformation process, the starting substrate of podophyllotoxin was biotransformed into 4′-demethylepipodophyllotoxin (product 1) with the demethylation of the methoxyl group at the 4′ position byGibberella fujikuroiSH-f13, which was screened out from Shennongjia prime forest humus soil (Hubei, China). 4′-Demethylepipodophyllotoxin (product 1) was then biotransformed into 4′-demethylpodophyllotoxone (product 2) with the oxidation of the hydroxyl group at the 4 position byAlternaria alternataS-f6, which was screened out from the gatheredDysosma versipellisplants in the Wuhan Botanical Garden, Chinese Academy of Sciences. Finally, 4′-demethylpodophyllotoxone (product 2) and ligustrazine were linked with a transamination reaction to synthesize the target product 4-TMP-DMEP (product 3) byAlternaria alternataS-f6. Compared with podophyllotoxin (i.e., a 50% effective concentration [EC50] of 529 μM), the EC50of 4-TMP-DMEP against the tumor cell line BGC-823 (i.e., 0.11 μM) was significantly reduced by 5,199 times. Simultaneously, the EC50of 4-TMP-DMEP against the normal human proximal tubular epithelial cell line HK-2 (i.e., 0.40 μM) was 66 times higher than that of podophyllotoxin (i.e., 0.006 μM). Furthermore, compared with podophyllotoxin (i.e., logP= 0.34), the water solubility of 4-TMP-DMEP (i.e., logP= 0.66) was significantly enhanced by 94%. For the first time, the novel compound 4-TMP-DMEP with superior antitumor activity was directionally synthesized from podophyllotoxin by the novel tandem biotransformation process developed in this work.


1994 ◽  
Vol 1994 (40) ◽  
pp. 33-37 ◽  
Author(s):  
Takumi YOSHIZAWA ◽  
Mika YAMASAKI ◽  
Naoki NANBA ◽  
Akihiro YAMASHITA ◽  
Susumu UEDA ◽  
...  

1982 ◽  
Vol 69 (3) ◽  
pp. 712-716 ◽  
Author(s):  
Ronald C. Coolbaugh ◽  
David R. Hell ◽  
Charles A. West

Author(s):  
B. E. Cross ◽  
R. H. B. Galt ◽  
J. R. Hanson ◽  
P. J. Curtis ◽  
John Frederick Grove ◽  
...  

Author(s):  

Abstract A new distribution map is provided for Cephalosporium sacchari[Gibberella fujikuroi var. subglutinans] Butler. Hosts: Sugarcane (Saccharum officinarum). Information is given on the geographical distribution in AFRICA, Madagascar, Réunion, Rhodesia, South Africa, Uganda, ASIA, India, Pakistan, Philippines, CENTRAL AMERICA, WEST INDIES, Barbados, Dominican Republic, Guadeloupe, Trinidad, NORTH AMERICA, Mexico, SOUTH AMERICA, Argentina, Brazil, Colombia.


Author(s):  
C. Booth

Abstract A description is provided for Gibberella fujikuroi var. subglutinans. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On several hosts of economic importance in the Gramineae; also on a wide range of hosts represented by the following families: Amaryllidaceae, Anacardiaceae, Bromeliaceae, Chenopodiaceae, Convolvulaceae, Cruciferae, Iridaceae, Leguminosae, Liliaceae, Malvaceae, Marantaceae, Musaceae, Palmae, Rosaceae, Rutaceae, Sterculiaceae (14: 708; 31: 515; 36: 501; 40: 89 and Herb. IMI). DISEASES: Causes a seedling blight, and root, stalk and kernel rot of maize; also on heads and stalks of sorghum associated with a foot and stem rot, and causing a stem rot and top rot of sugar-cane ('pokkah boeng'). Other records include a wilt of Crotalaria, a heart rot of leaves of banana and Manila hemp, and fruit rot of banana, cacao and pineapple. There appear to be no references to pathogenicity to rice. Also entomogenous on cereal stem borer larvae and other insects (27: 71; 33: 382; 38: 141, 740). GEOGRAPHICAL DISTRIBUTION: Africa (Central African Republic, Congo, Ghana, Ivory Coast, Kenya, Mauritius, Morocco, Reunion, Sierra Leone, South Africa, Southern Rhodesia, Tanganyika, Uganda); Asia (Formosa (Taiwan), Hong Kong, India, Java, Indo-China, Philippines, Syria); Australasia (Hawaii, New South Wales, New Zealand, Victoria); Europe (Czechoslovakia, Germany,? Italy, Poland, Romania); Central America & West Indies (French Antilles, Honduras, Trinidad); North America (Canada, United States); South America (Argentina, Peru). (CMI Map 191). TRANSMISSION: Both seed and soil-borne. Air-borne ascospores produced from perithecia on over-wintered plant debris or on dead stalks of sugar-cane at the beginning of the rainy season are also important sources of infection (30: 344). The pathogen may also be disseminated on pupae and adults of cereal stem borers and their parasites in sugar-cane (33: 382).


1988 ◽  
Vol 27 (2) ◽  
pp. 628-629 ◽  
Author(s):  
W. David Nes ◽  
Eugene E. Van Tamelen ◽  
E.J. Leopold
Keyword(s):  

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