Alkaline phosphatase activity and nucleic acids during embryonic development of Amblystoma punctatum at different temperatures

1952 ◽  
Vol 121 (3) ◽  
pp. 489-504 ◽  
Author(s):  
Edith J. Krugelis ◽  
J. S. Nicholas ◽  
Marjorie E. Vosgian
Keyword(s):  
Alkaline Phosphatase ◽  
Embryonic Development ◽  
Nucleic Acids ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Different Temperatures

The Localization of Alkaline Phosphatase during the Post-embryonic Development of Drosophila melanogaster

1950 ◽  
Vol s3-91 (13) ◽  
pp. 89-105
Author(s):  
T. YAO
Keyword(s):  
Alkaline Phosphatase ◽  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Larval Instar ◽  
Morphological Evidence ◽  
Simultaneous Increase ◽  
Endocrine Organs ◽  
Pericardial Cells

1. The localization of alkaline phosphatase during the post-embryonic development of Drosophila melanogaster has been described. 2. In the larvae, nuclear phosphatase is always demonstrable, but cytoplasmic phosphatase shows a more restricted distribution. Salivary glands, mid-gut, Malpighian tubes, and pericardial cells are richest in cytoplasmic phosphatase. 3. The larva prior to puparium formation is characterized by a decrease of alkaline phosphatase in the internal organs with a simultaneous increase in the hypodermis. 4. The phosphatase data support the view that the prepupa is actually an intrapuparial larval instar. 5. Pupation is accompanied, by a very noticeable increase of alkaline phosphatase which is mainly confined to the cytoplasm. The high enzyme activity is maintained for the first day and a half after head eversion: there is a subsequent decline until at the time of emergence most organs are inactive. However, certain organs retain their alkaline phosphatase activity. 6. As in embryogenesis, alkaline phosphatase seems to be more concerned with histo-differentiation than with chemo-differentiation. 7. Alkaline phosphatase (and also acid phosphatase) actively participates in the process of histolysis or cellular degeneration. 8. The alkaline phosphatase activity of the pericardial cells, together with other morphological evidence, indicates that these cells are endocrine organs which play important roles in Drosophila metamorphosis. 9. Cytochemical evidence suggests that alkaline phosphatase in Drosophila is probably playing a part in the carriage of organic substances across the membrane barrier.

PHOSPHOMONOESTERASE ACTIVITY IN THE LIFE-CYCLE STAGES OF SCHISTOCERCA GREGARIA

1968 ◽  
Vol 100 (6) ◽  
pp. 649-655 ◽  
Author(s):  
S. N. H. Naqvi ◽  
Shahid H. Ashrafi ◽  
M. A. H. Qadri
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Embryonic Development ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Schistocerca Gregaria ◽  
Adult Males ◽  
First Instar ◽  
Almost All

AbstractThe acid and alkaline phosphatase activity was measured in the developing egg and in the alimentary canal of aging nymphs as well as adult males and females of different ages. Para-nitrophenol was used as colorimetric standard and disodium p-nitrophenyl phosphate as substrate. Activity was measured in terms of micromoles of p-nitrophenol liberated from the substrate as a result of enzyme action.Acid phosphatase activity was noticed to increase with the embryonic development and was higher than in the case of alkaline phosphatase. The alkaline phosphatase activity was lowest in the freshly laid egg, but increased more sharply than acid phosphatase during embryonic development.The activity of both the acid and alkaline phosphatases was highest in the first instar and declined gradually to the fifth instar. The activity of acid phosphatase was higher than alkaline phosphatase in all stages except the first instar where it was almost equal. The activity of both the enzymes was higher during the intermoulting period and declined at each moult indicating a hormone–enzyme relationship.In adults, activity of both the enzymes increased up to the maturation period after which the activity gradually decreased. Acid phosphatase activity was generally higher in males whereas alkaline phosphatase activity was generally higher in females. In almost all cases, the acid phosphatase activity was found to be higher than the alkaline phosphatase.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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