Scanning electron microscopy of primordial germ cells in early chick embryos

1978 ◽  
Vol 206 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Hsin-Yi Lee ◽  
Robert G. Nagele ◽  
Mark M. Goldstein
1984 ◽  
Vol 13 (3) ◽  
pp. 193-196 ◽  
Author(s):  
V. J. Götzens ◽  
M. Gimeno ◽  
L. Dominguez ◽  
S. Climent

Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.


1977 ◽  
Vol 150 (3) ◽  
pp. 291-300 ◽  
Author(s):  
Marjorie A. England ◽  
Jennifer Wakely

1998 ◽  
Vol 8 (3) ◽  
pp. 396-399 ◽  
Author(s):  
Jörg Männer ◽  
Wolfgang seidl ◽  
Gerd Steding

AbstractChick embryos are frequently used as animal models when researching the developing heart. In the past, every attempt to induce complete transposition (the combination of concordant arrioventricular and discordant ventriculo-arterial connections) failed in chicks, suggesting that it might be impossible to develop a chicken modle for this malformation. We demonstrate, to the best of our knowledge, the first well-documented case of complete transpositon occourrine in the chick


1973 ◽  
Vol 146 (4) ◽  
pp. 543-552 ◽  
Author(s):  
W. Breipohl ◽  
N. Bornfeld ◽  
G. J. Bijvank ◽  
H. Laugwitz ◽  
M. Pfautsch

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