Heat shock protein 70 gene expression in intact salamanders (Eurycea bislineata) in response to calibrated heat shocks and to high temperatures encountered in the field

Heat shock protein 70 (HSP70) is a chaperon that stabilizes unfolded or partially folded proteins, preventing inappropriate inter- and intramolecular interactions. Here, we examined the developmental competence of in vitro matured oocytes exposed to heat stress with or without HSP70. Bovine oocytes were matured for 24 h at 39 °C without (group C39) or with HSP70 (group H39) and at 41 °C for the first 6 h, followed by 16 h at 39 °C with (group H41) or without HSP70 (group C41). After insemination, zygotes were cultured for 9 days at 39 °C. Cleavage and embryo yield were assessed 48 h post insemination and on days 7, 8, 9, respectively. Gene expression was assessed by RT-PCR in oocytes, cumulus cells and blastocysts. In C41, blastocysts formation rate was lower than in C39 and on day 9 it was lower than in H41. In oocytes, HSP70 enhanced the expression of three HSP genes regardless of incubation temperature. HSP70 at 39 °C led to tight coordination of gene expression in oocytes and blastocysts, but not in cumulus cells. Our results imply that HSP70, by preventing apoptosis, supporting signal transduction, and increasing antioxidant protection of the embryo, protects heat stressed maturing bovine oocyte and restores its developmental competence.

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Effect of heat shock protein 70 homologue DNAK on gene expression of penaeidins, serine proteinase, prophenoloxidase and transglutaminase in hemocytes of Litopenaeus vannamei

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2013.03.068 ◽

2013 ◽

Vol 34 (6) ◽

pp. 1656

Author(s):

B Hu ◽

P Sorgeloos ◽

P Bossier

Keyword(s):

Gene Expression ◽

Heat Shock ◽

Heat Shock Protein ◽

Litopenaeus Vannamei ◽

Heat Shock Protein 70 ◽

Serine Proteinase

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Induction of Heat Shock Protein Genes is the Hallmark of Egg Heat Tolerance in Agasicles hygrophila (Coleoptera: Chrysomelidae)

Journal of Economic Entomology ◽

10.1093/jee/toaa105 ◽

2020 ◽

Vol 113 (4) ◽

pp. 1972-1981

Author(s):

Dong Jia ◽

Yan-Hong Liu ◽

Bin Zhang ◽

Zhou-Yu Ji ◽

Yuan-Xin Wang ◽

...

Keyword(s):

Gene Expression ◽

Heat Shock ◽

Heat Shock Protein ◽

High Temperatures ◽

Heat Tolerance ◽

Invasive Plant ◽

Alternanthera Philoxeroides ◽

Alligator Weed ◽

Natural Habitats ◽

Hsp Genes

Abstract Insects are ecotothermic organisms. Their development, survival, reproduction as well as distribution and abundance are affected by temperature. Heat shock protein (HSP) gene expression is closely associated with temperature variation and influences the adaptation of organisms to adverse environments. The beetle Agasicles hygrophila has successfully been used for biological control of the invasive plant alligator weed (Alternanthera philoxeroides). As A. hygrophila populations are substantially inhibited by high temperatures in the summer, increasing global temperatures may limit the efficacy of this control agent. We previously established that A. hygrophila eggs have low tolerance to heat and this factored into the decreased numbers of A. hygrophila beetles at temperatures of 37.5°C and above. Here, we identified 26 HSP genes in A. hygrophila and examined the relationship between the transcript levels of these genes and heat tolerance. The temperature at which the expression of these 21 HSP genes peaked (Tpeak) was 37.5°C, which is in line with the limit of the high temperatures that A. hygrophila eggs tolerate. Therefore, we speculate that the Tpeak of HSP gene expression in eggs indicates the upper limit of temperatures that A. hygrophila eggs tolerate. This study identifies HSP genes as potential robust biomarkers and emphasizes that determining species’ heat tolerance in their natural habitats remains an important consideration for biocontrol. HSP gene expression data provide information about a species’ heat tolerance and may be used to predict its geographical distribution.

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