CRISPR/Cas9 mediated GFP‐human dentin matrix protein 1 (DMP1) promoter knock‐in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation

2020 ◽  
Vol 22 (12) ◽  
Author(s):  
Fahimeh Shahabipour ◽  
Reza Kazemi Oskuee ◽  
Mohammad Ali Shokrgozar ◽  
Hojjat Naderi‐Meshkin ◽  
Lena Goshayeshi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteoblast Differentiation ◽  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Rosa26 Locus ◽  
Dentin Matrix Protein ◽  
Human Dentin ◽  
Dentin Matrix

scholarly journals Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells

2019 ◽  
Vol 23 ◽  
pp. e00348 ◽  
Author(s):  
Aktsar Roskiana Ahmad ◽  
Pornjira Kaewpungsup ◽  
Narach Khorattanakulchai ◽  
Kaewta Rattanapisit ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Matrix Protein ◽  
Periodontal Ligament Cells ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Human Periodontal Ligament Cells ◽  
Human Dentin ◽  
Dentin Matrix

The recombinant human dentin matrix protein 1-coated titanium and its effect on the attachment, proliferation and ALP activity of MG63 cells

2012 ◽  
Vol 23 (11) ◽  
pp. 2717-2726 ◽  
Author(s):  
Xibo Pei ◽  
Lanlan Pan ◽  
Fenglin Cui ◽  
Rui He ◽  
Hong Bao ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Alp Activity ◽  
Mg63 Cells ◽  
Human Dentin ◽  
Dentin Matrix

scholarly journals Immunohistochemical localization of dentin matrix protein 1 in human dentin

10.4081/1219 ◽  
2009 ◽  
Vol 52 (4) ◽  
pp. 215 ◽  
Author(s):  
G Orsini ◽  
A Ruggeri ◽  
A Mazzoni ◽  
F Nato ◽  
M Falconi ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Immunohistochemical Localization ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Human Dentin ◽  
Dentin Matrix

scholarly journals Transcriptional Regulation of Dentin Matrix Protein 1 by JunB and p300 during Osteoblast Differentiation

2004 ◽  
Vol 279 (43) ◽  
pp. 44294-44302 ◽  
Author(s):  
Karthikeyan Narayanan ◽  
Rampalli Srinivas ◽  
Mathew Craig Peterson ◽  
Amsaveni Ramachandran ◽  
Jianjun Hao ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Osteoblast Differentiation ◽  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Dentin Matrix

scholarly journals Functional derivatives of human dentin matrix protein 1 modulate morphology of calcium carbonate crystals

2020 ◽  
Vol 34 (5) ◽  
pp. 6147-6165
Author(s):  
Aleksandra Porębska ◽  
Mirosława Różycka ◽  
Rafał Hołubowicz ◽  
Zbigniew Szewczuk ◽  
Andrzej Ożyhar ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Functional Derivatives ◽  
Human Dentin ◽  
Dentin Matrix ◽  
Derivatives Of

scholarly journals Recombinant Human Dentin Matrix Protein 1 (hDMP1) Expressed in Nicotiana benthamiana Potentially Induces Osteogenic Differentiation

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 566 ◽  
Author(s):  
Aktsar Roskiana Ahmad ◽  
Pornjira Kaewpungsup ◽  
Narach Khorattanakulchai ◽  
Kaewta Rattanapisit ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Nicotiana Benthamiana ◽  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Human Dentin ◽  
Dentin Matrix

Inductive molecules are critical components for successful bone tissue engineering. Dentin matrix protein-1 (DMP1), a non-collagenous protein in the bone matrix, has been shown to play roles in osteogenic differentiation and phosphate homeostasis. This study aimed to produce recombinant human dentin matrix protein-1 (hDMP1) in Nicotiana benthamiana and investigated the ability of this plant-produced DMP1 to induce osteogenesis in human periodontal ligament stem cells (hPDLSCs). The hDMP1 gene was cloned into the geminiviral vector for transient expression in N. benthamiana. We found that hDMP1 was transiently expressed in N. benthamiana leaves and could be purified by ammonium sulphate precipitation followed by nickel affinity chromatography. The effects of hDMP1 on the induction of cell proliferation and osteogenic differentiation were investigated. The results indicated that plant-produced hDMP1 could induce the cell proliferation of hPDLSCs and increase the expression levels of osteogenic genes, including osterix (OSX), type I collagen (COL1), bone morphogenetic protein-2 (BMP2), and Wnt3a. Moreover, the plant-produced hDMP1 promoted calcium deposition in hPDLSCs as determined by alizarin red S staining. In conclusion, our results indicated that plant-produced hDMP1 could induce osteogenic differentiation in hPDLSCs and could potentially be used as a bone inducer in bone tissue engineering.

Binding of two nuclear factors to a novel silencer element in human dentin matrix protein 1 (DMP1) promoter regulates the cell type-specific DMP1 gene expression

2004 ◽  
Vol 92 (2) ◽  
pp. 332-349 ◽  
Author(s):  
Shuo Chen ◽  
Natalyia Inozentseva-Clayton ◽  
Juan Dong ◽  
Ting Ting Gu ◽  
Mary MacDougall
Keyword(s):  
Gene Expression ◽  
Matrix Protein ◽  
Cell Type ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Nuclear Factors ◽  
Human Dentin ◽  
Cell Type Specific ◽  
Dentin Matrix ◽  
Silencer Element

scholarly journals Dentin matrix protein 1 (DMP1) expression in developing human teeth

2009 ◽  
Vol 20 (5) ◽  
pp. 365-369 ◽  
Author(s):  
Elizabeth Ferreira Martinez ◽  
Luciana Alves Herdy da Silva ◽  
Cristiane Furuse ◽  
Ney Soares de Araújo ◽  
Vera Cavalcanti de Araújo
Keyword(s):  
Dental Pulp ◽  
Matrix Protein ◽  
Enamel Organ ◽  
Dentin Matrix Protein 1 ◽  
Tooth Formation ◽  
Dentinal Tubules ◽  
Dental Lamina ◽  
Dentin Matrix Protein ◽  
Dental Papilla ◽  
Dentin Matrix

Dentin matrix protein 1 (DMP1) is an acidic phosphoprotein that plays an important role in mineralized tissue formation by initiation of nucleation and modulation of mineral phase morphology. The purpose of the present study was to examine the immunoexpression of DMP1 in tooth germs of 7 human fetuses at different gestational ages (14, 16, 19, 20, 21, 23 and 24 weeks) comparing with completed tooth formation erupted teeth. The results showed the presence of DMP1 in the dental lamina, as well as in the cells of the external epithelium, stellate reticulum and stratum intermedium of the enamel organ. However, in the internal dental epithelium, cervical loop region and dental papilla some cells have not labeled for DMP1. In the crown stage, DMP1 was expressed in the ameloblast and odontoblast layer, as well as in the dentinal tubules of coronal dentin near the odontoblast area. Erupted teeth with complete tooth formation exhibited immunolabeling for DMP1 only in the dentinal tubules mainly close to the dental pulp. No staining was observed in the enamel, predentin or dental pulp matrix. DMP1 is present in all developing dental structures (dental lamina, enamel organ, dental papilla) presenting few immunoexpression variations, with no staining in mineralized enamel and dentin.

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