Biochemical Characterization of Interleukin 1 From a Human Monocytic Cell Line

1985 ◽  
Vol 37 (5) ◽  
pp. 511-518 ◽  
Author(s):  
Teresa Krakauer
10.1251/bpo92 ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 220-225 ◽  
Author(s):  
Neora Pick ◽  
Scott Cameron ◽  
Dorit Arad ◽  
Yossef Av-Gay

2019 ◽  
Author(s):  
Ana Neves-Costa ◽  
Dora Pedroso ◽  
Luis F Moita

Abstract This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune responsive cells. The cell line used was the human monocytic cell line THP-1, an adherent cell type with many known applications in in vitro studies of innate immunity. The comet assay is a robust procedure that allows the accurate and reproducible quantification of DNA damage. Here we describe not only the comet assay step-by-step protocol, but also some important aspects related to troubleshooting.


2011 ◽  
Vol 14 (3) ◽  
pp. 240-246 ◽  
Author(s):  
Nam-Seok Kim ◽  
Seung-Il Jeong ◽  
Byung-Soon Hwang ◽  
Young-Eun Lee ◽  
Shin-Ho Kang ◽  
...  

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