Morphological patterns of cell death in ovarian follicles of primary and secondary growth and postovulatory follicle complex of the annual killifish Millerichthys robustus (Cyprinodontiformes: Cynolebiidae)

2019 ◽  
Vol 280 (11) ◽  
pp. 1668-1681
Author(s):  
Omar Domínguez‐Castanedo ◽  
Mari Carmen Uribe ◽  
Tessy M. Muñoz‐Campos
Keyword(s):  
Cell Death ◽  
Secondary Growth ◽  
Ovarian Follicles ◽  
Annual Killifish ◽  
Postovulatory Follicle ◽  
Morphological Patterns

scholarly journals Cork cells in cork oak periderms undergo programmed cell death and proanthocyanidin deposition

2021 ◽  
Author(s):  
Vera Inácio ◽  
Carolina Lobato ◽  
José Graça ◽  
Leonor Morais-Cecílio
Keyword(s):  
Cell Death ◽  
Cell Wall ◽  
Programmed Cell Death ◽  
Secondary Growth ◽  
Cork Oak ◽  
Comprehensive Overview ◽  
Highly Active ◽  
Transmission Electron ◽  
Traumatic Wound ◽  
The Impact

Abstract Vascular plants with secondary growth develop a periderm mostly composed of dead suberized cork cells to face environmental hostile conditions. Cork oak has a highly active and long-living phellogen forming a remarkably thick periderm that is periodically debarked for industrial purposes. This wounding originates the quick formation of a new traumatic periderm, making cork oak an exceptional model to study the first periderm differentiation during normal development in young sprigs and traumatic (wound) periderm formation after debarking. Here, we studied the poorly known first periderm differentiation steps that involve cell wall suberization, polyphenolic accumulation and programmed cell death (PCD) by combining transmission electron microscopy, histochemical and molecular methods in periderms from young sprigs. These processes were further compared with traumatic periderms formed after wounding using molecular and histochemical techniques, such as the polyphenolic accumulation. In the first periderms from young sprigs, four distinct differentiation stages were defined according to the presence of PCD morphological features. First young and traumatic periderms showed an upregulation of genes related to suberin biosynthesis, proanthocyanidins biosynthesis and transport, autophagy, and PCD. Traumatic periderms revealed an overall upregulation of these genes, likely resulting from ontogeny differences and distinct phellogen origin associated with a faster metabolism, highlighting the impact of wounding on phellogen activity after debarking. First periderms from young sprigs showed gradual accumulation of proanthocyanidins in the vacuoles throughout PCD stages until total filled lumens, whereas in traumatic periderms, these compounds were found cell wall linked in already empty cells. This work enabled a comprehensive overview of the cork cells differentiation processes contributing to deepening the knowledge of the fundamental ontogenic program of this protective tissue, which is also a unique forest product, constituting the basis of a sustainable and profitable industry.

scholarly journals Matrix Metalloproteinase Inhibition Facilitates Cell Death in Intracerebral Hemorrhage in Mouse

2007 ◽  
Vol 28 (4) ◽  
pp. 752-763 ◽  
Author(s):  
Mark Grossetete ◽  
Gary A Rosenberg
Keyword(s):  
Cell Death ◽  
Matrix Metalloproteinase ◽  
Intracerebral Hemorrhage ◽  
Secondary Growth ◽  
Death Receptors ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Gelatinolytic Activity ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Null Mouse ◽  
Tissue Inhibitor

Intracerebral hemorrhage (ICH) initiates an inflammatory response with secondary growth of hemorrhage and cell death. Matrix metalloproteinase (MMP) gelatinolytic activity is increased in ICH, and synthetic inhibitors to MMPs reduce edema and hemorrhage size. Recently, we found that tissue inhibitor of metalloproteinase-3 (TIMP-3) is elevated after ischemia and colocalizes with TUNEL (terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate-biotin nick end-labeled)-labeled cells. Tissue inhibitor of metalloproteinase-3 promotes neuronal apoptosis in vitro by blocking the shedding of the tumor necrosis factor (TNF) superfamily of death receptors/ligands by stromelysin-1 (MMP-3). However, the effect of TIMP-3 and synthetic MMP inhibitors on cell death in ICH is unclear. Therefore, we used the collagenase-induced intracerebral hemorrhage (CIH) model in Timp-3 knockout and C57Bl/6 wild-type mice to study MMP expression, hemorrhage volume, and cell death. Real-time PCR showed an increase in Mmp-3 mRNA in CIH, but similar Mmp-2 and -9 mRNA expression levels in CIH and saline-injected mice. Protein levels of pro and cleaved MMP-3 were increased in CIH, and zymographic gelatinolytic activity of MMP-9 was elevated after CIH at 72 h, suggesting an exogenous source. Apoptosis was shown by increased caspase-3 levels at 2 and 72 h, and active caspase-8 by 2 and 24 h. The Timp-3 null mouse and wild types had similar hemorrhage sizes and TUNEL-labeled cells. Unexpectedly, the broad-spectrum MMP inhibitor BB-94 increased hemorrhage size and TUNEL-labeled cells. Our results fail to implicate TIMP-3 in apoptosis in CIH, but show that BB-94 increased apoptosis in CIH, possibly by blocking shedding of TNF death receptors and/or their ligands.

scholarly journals Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (Sigmodon hispidus)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1768
Author(s):  
Md Rashedul Islam ◽  
Osamu Ichii ◽  
Teppei Nakamura ◽  
Takao Irie ◽  
Akio Shinohara ◽  
...  
Keyword(s):  
Cell Death ◽  
Strain Differences ◽  
Inbred Strains ◽  
Ovarian Follicles ◽  
Sigmodon Hispidus ◽  
Cotton Rat ◽  
Ovarian Histology ◽  
Ovarian Morphology ◽  
Cotton Rats ◽  
Single Nucleus

Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, Sigmodon hispidus). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences.

scholarly journals Monoclonal Antibodies Recognize a Novel Cell Death Receptor and a Decoy Receptor on Granulosa Cells of Porcine Ovarian Follicles.

2001 ◽  
Vol 18 (1) ◽  
pp. 20-32 ◽  
Author(s):  
Noboru Manabe ◽  
Akira Myoumoto ◽  
Chiemi Tajima ◽  
Mizuho Nakayama ◽  
Misuzu Yamaguchi ◽  
...  
Keyword(s):  
Cell Death ◽  
Monoclonal Antibodies ◽  
Granulosa Cells ◽  
Death Receptor ◽  
Ovarian Follicles ◽  
Decoy Receptor ◽  
Cell Death Receptor

An Ultrastructural Study of the Proliferation Patterns of the Reserve Cells of the Human Cervix Uteri

1972 ◽  
Vol 30 ◽  
pp. 80-81
Author(s):  
Takanori Sohda ◽  
Hiroshi Saito ◽  
Goro Asano ◽  
Katsunari Fukushi ◽  
Katsuya Suzuki ◽  
...  
Keyword(s):  
Ultrastructural Study ◽  
Age Groups ◽  
Point Of View ◽  
University Hospital ◽  
Cervix Uteri ◽  
Morphological Aspect ◽  
Functional Aspect ◽  
Pathological Conditions ◽  
Human Cervix ◽  
Morphological Patterns

Recently, the functional aspect as well as morphological aspect of the reserve cells in the cervix uteri drew much attention in view of the carcinogenesis in squamocolumunar junction. In this communication, the authors elucidate the ultrastructural features of the reserve cells in patients of various age groups visiting our university hospital and affiliated hospital.From conventional light microscopic point of view, the reserve cells tend to be pronounced in various pathological conditions, such as the persisting inflammation, proliferative disorders and irritation of hormones. The morphological patterns of the reserve cells from various stage and degree of irritation were observed.

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