Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences

1988 ◽  
Vol 26 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Ronald Melki ◽  
Bernadette Khoury ◽  
Francois Catalan
Keyword(s):  
Human Papillomavirus ◽  
Nucleic Acid ◽  
Dna Sequences ◽  
Spot Hybridization ◽  
Nucleic Acid Spot Hybridization ◽  
Papillomavirus Dna

scholarly journals Prevalence of human papillomavirus DNA sequences in an area with very high incidence of cervical carcinoma

1994 ◽  
Vol 70 (4) ◽  
pp. 694-696 ◽  
Author(s):  
CC Pao ◽  
SM Kao ◽  
G-C Tang ◽  
K Lee ◽  
J Si ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Carcinoma ◽  
Dna Sequences ◽  
High Incidence ◽  
Papillomavirus Dna ◽  
Very High

scholarly journals Presence of human papillomavirus DNA sequences in cervical intraepithelial neoplasia.

BMJ ◽  
1983 ◽  
Vol 287 (6395) ◽  
pp. 784-788 ◽  
Author(s):  
D J McCance ◽  
P G Walker ◽  
J L Dyson ◽  
D V Coleman ◽  
A Singer
Keyword(s):  
Human Papillomavirus ◽  
Cervical Intraepithelial Neoplasia ◽  
Dna Sequences ◽  
Intraepithelial Neoplasia ◽  
Papillomavirus Dna

Human papillomavirus DNA sequences in esophagus squamous cell carcinoma

1994 ◽  
Vol 107 (1) ◽  
pp. 128-136 ◽  
Author(s):  
Kazumi Togawa ◽  
Kasimir Jaskiewicz ◽  
Hiroshi Takahashi ◽  
Stephen J. Meltzer ◽  
Anil K. Rustgi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Dna Sequences ◽  
Esophagus Squamous Cell Carcinoma ◽  
Papillomavirus Dna

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

2009 ◽  
Vol 64 (9-10) ◽  
pp. 711-716 ◽  
Author(s):  
Kuraba Gopal ◽  
Sundeep Sudarsan ◽  
Venati Gopi ◽  
Latchireddy Naram Naidu ◽  
Maniyaram Ramaiah ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sweet Orange ◽  
Pcr Amplification ◽  
Positive Control ◽  
Infected Tissue ◽  
Citrus Greening Disease ◽  
Pcr Product ◽  
Spot Hybridization ◽  
Total Dna ◽  
Nucleic Acid Spot Hybridization

Polymerase chain reaction (PCR) amplification with primers specific to the rDNA region successfully amplified the 1160-bp DNA fragment from a Huanglongbing (HLB)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. The PCR product of 1160-bp was used as probe labeled with biotin for detection of the HLB pathogen in the nucleic acid spot hybridization (NASH) test. It was found that the HLB pathogen could be detected up to 1:100 dilution in HLB-infected tissue. Total DNA extracted from HLB-infected tissue was diluted 2-fold as 900 ng in TE buffer and spotted on a nitrocellulose membrane. Strong signals were observed up to 225 ng of DNA dilution, whereas a moderate signal was recorded at 112 ng. No hybridization signal was observed in the healthy samples, while strong signals were observed in the positive control

Human Papillomavirus DNA Sequences in Cell Lines Derived from Head and Neck Squamous Cell Carcinomas

1991 ◽  
Vol 104 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Carol R. Bradford ◽  
Susan E. Zacks ◽  
Elliott J. Androphy ◽  
Lucie Gregoire ◽  
Wayne D. Lancaster ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Dna Sequences ◽  
Squamous Cell Carcinomas ◽  
Papillomavirus Dna
Sign in / Sign up

Export Citation Format

Share Document