Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil

2010 ◽  
Vol 82 (8) ◽  
pp. 1400-1405 ◽  
Author(s):  
Luiza A. Castro-Jorge ◽  
Paula R.L. Machado ◽  
Camila A. Fávero ◽  
Marcos C. Borges ◽  
Luzia M.R. Passos ◽  
...  
Keyword(s):  
Virus Infection ◽  
Early Diagnosis ◽  
Dengue Virus ◽  
Clinical Evaluation ◽  
Dengue Virus Infection ◽  
Capture Elisa ◽  
Antigen Capture Elisa ◽  
Antigen Capture ◽  
Ns1 Antigen

scholarly journals Dengue NS1 Antigen: A Tool in Early Detection of Dengue Virus Infection

2014 ◽  
Vol 15 (1) ◽  
pp. 28-30 ◽  
Author(s):  
Rama Biswas ◽  
Fazle Rabbi Mohammed ◽  
Poly Sengupta ◽  
Hasan Shahrear Ahmed ◽  
Md. Motlabur Rahman ◽  
...  
Keyword(s):  
Virus Infection ◽  
Early Diagnosis ◽  
Dengue Virus ◽  
Complete Blood Count ◽  
Tertiary Care ◽  
Care Hospital ◽  
Igg Antibody ◽  
Dengue Virus Infection ◽  
Igm Antibody ◽  
Ns1 Antigen

Objectives: This aim of the study was to evaluate the efficacy of NS1 antigen assay for early diagnosis of dengue virus infection in a tertiary care hospital. Methods: This cross sectional study was carried out in medicine unit of Square hospital from May to August 2013. Total 100 patients with dengue fever were included. Complete blood count, alanine aminotransferase (ALT), aspartate aminotransferase (AST), Dengue NS1 antigen and IgM and IgG antibodies of dengue virus were done in all cases. Results: Of the 100 sera tested, 75% were positive for dengue virus infection based on dengue NS1 antigen, IgM antibody and IgG antibody. Dengue NS1 antigen and IgM, IgG antibody were able to detect dengue virus infection between day 1 to day 8 in 92% of samples, 86.7% of samples and 82.6% of samples respectively. Sixty nine percent (69%) were found positive for dengue NS1 antigen, 65% were IgM positive and 62% were IgG positive. Based on the dengue NS1 antigen and IgM antibody combination, 74% were positive for dengue virus infections. Sensitivity of Dengue NS1 antigen was 92.3% and specificity of 74.28% in comparison to IgM antibody. Based on the antigen and IgG antibody combination, detection rate increased to 75%. Sensitivity of dengue NS1 antigen was 90.3% and specificity of 65.8% in comparison to IgG antibody. Conclusion: Dengue NS1 antigen is a useful, sensitive and specific test for early diagnosis of dengue virus infection and it improves diagnostic efficiency in combination with antibody test.DOI: http://dx.doi.org/10.3329/jom.v15i1.19856 J Medicine 2014; 15: 28-30

scholarly journals Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using NS1 Antigen Detection in Human Serum

2008 ◽  
Vol 2 (8) ◽  
pp. e280 ◽  
Author(s):  
Philippe Dussart ◽  
Laure Petit ◽  
Bhety Labeau ◽  
Laetitia Bremand ◽  
Alexandre Leduc ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Human Serum ◽  
Antigen Detection ◽  
Dengue Virus Infection ◽  
Ns1 Antigen

scholarly journals NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

2018 ◽  
Vol 17 (4) ◽  
pp. 669-673
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
AKM Muraduzzaman ◽  
Tahmina Shirin
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Medical Science ◽  
Cost Effective ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Medical College ◽  
Virus Serotype ◽  
Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

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