Practical thin layer chromatography techniques for diaminopimelic acid and whole cell sugar analyses in the classification of environmental actinomycetes

2006 ◽  
Vol 46 (3) ◽  
pp. 243-249 ◽  
Author(s):  
Renate Schön ◽  
Ingrid Groth
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Diaminopimelic Acid ◽  
Whole Cell ◽  
Layer Chromatography

scholarly journals Rapid method for the detection and identification of mycolic acids in aerobic actinomycetes and related bacteria

1976 ◽  
Vol 4 (3) ◽  
pp. 284-287
Author(s):  
S T Hecht ◽  
W A Causey
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Clinical Laboratory ◽  
Diaminopimelic Acid ◽  
Whole Cell ◽  
Mycolic Acids ◽  
Detection And Identification ◽  
Layer Chromatography ◽  
Aerobic Actinomycetes

A rapid method for the identification of lipids characteristic of the genera Corynebacterium, Mycobacterium, Nocardia, and the "rhodochrous group" has been developed. Modifications of previously described methods make this procedure suitable for use in the clinical laboratory. Thin-layer chromatography is used to demonstrate the presence of the lipid characteristic of Nocardia spp. (type A) in some corynebacteria, nocardias, and members of the "rhodochrous group." Precipitation in ether and ethanol is used to demonstrate the presence of mycobacterial mycolic acids. Since this procedure can be carried out in less than 2 days and the lipids are extracted from the same batch of cells grown for diaminopimelic acid and whole-cell sugar analyses, it can readily be added to the battery of tests performed in reference laboratories that deal with aerobic actinomycetes and related bacteria.

The use of thin-layer chromatography for the separation and identification of phytoplankton pigments

1965 ◽  
Vol 45 (3) ◽  
pp. 583-591 ◽  
Author(s):  
J. P. Riley ◽  
T. R. S. Wilson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phaeodactylum Tricornutum ◽  
Isochrysis Galbana ◽  
Marine Phytoplankton ◽  
Rapid Separation ◽  
Systematic Classification ◽  
Layer Chromatography ◽  
Natural Phytoplankton

Thin-layer chromatography has been used for the rapid separation of microgram amounts of pigments of marine phytoplankton, both from cultures and from the sea. The separation is carried out on plates coated with silica gel; the chromatogram is developed with a solvent consisting of petroleum ether (b.p. 60–80°), ethyl acetate and diethylamine in the ratio 58:30:12 by volume. The separated individual pigments may be eluted from the plate and characterized by their absorption spectra. The pigments of the following phytoplankton have been examined: Dunaliella primolecta, Phaeodactylum tricornutum, Isochrysis galbana, Dicrateria inornata, Coccolithus huxleyi, and Peridinium trochoidium.IntroductionA knowledge of the component pigments of phytoplankton is of value in the systematic classification of these organisms and in the study of the mechanism of photosynthesis. Very little is known about the amounts of the lesser phyto-plankton pigments associated with the natural phytoplankton crop in the sea, but a considerable amount of work has been carried out on the measurement of chlorophylls a, b and c and of carotenoids for the estimation of the plant bio-mass in the sea. These determinations are usually carried out by modifications of the poly-chromatic spectrophotometric method originally described by Richards with Thompson (1952) and modified by Creitz & Richards (1955) (see also Parsons & Strickland, 1963). While these procedures give a satisfactory measure of chlorophylls a and b, their precision for chlorophyll c and carotenoids is poor; furthermore, no resolution of carotenes from xanthophylls can be attained; nor can any indication of the presence of unusual pigments be obtained.

scholarly journals Classification of Mycobacterium farcinogenes and Mycobacterium senegalense by Immunodiffusion and Thin-layer Chromatography of Long-chain Components

Microbiology ◽  
1982 ◽  
Vol 128 (6) ◽  
pp. 1299-1307
Author(s):  
M. Ridell ◽  
M. Goodfellow ◽  
D. E. Minnikin ◽  
S. M. Minnikin ◽  
I. G. Hutchinson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography ◽  
Long Chain

scholarly journals A scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis

1975 ◽  
Vol 2 (1) ◽  
pp. 55-61
Author(s):  
V P Kurup ◽  
J N Fink
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Hypersensitivity Pneumonitis ◽  
Diaminopimelic Acid ◽  
Biochemical Tests ◽  
Chromatography Method ◽  
Thermophilic Actinomycetes ◽  
Saccharomonospora Viridis ◽  
Microscopic Morphology ◽  
Layer Chromatography

A scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. Eighty strains, 10 Micropolyspora faeni, 6 Saccharomonospora viridis, 52 Thermoactinomyces candidus, 7 T. vulgaris, 4 T. sacchari, and 1 T. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. In addition to the cultural and microscopic morphology in various media, each strain was subjected to an array of biochemical tests. These tests included decomposition of tyrosine, xanthine, hypoxanthine, gelatin, casein, esculin, and arbutin. Using a rapid thin-layer chromatography method, the isomer of diaminopimelic acid and sugar in the whole cell hydrolysate were studied. The thermophilic actinomycetes can be identified in a reasonable period of time using a combination of all these tests.

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