ABSTRACTSuccinoyl trehalose lipids (STLs) are promising glycolipid biosurfactants produced fromn-alkanes that are secreted byRhodococcusspecies bacteria. These compounds not only exhibit unique interfacial properties but also demonstrate versatile biochemical actions. In this study, three novel types of genes involved in the biosynthesis of STLs, including a putative acyl coenzyme A (acyl-CoA) transferase (tlsA), fructose-bisphosphate aldolase (fda), and alkane monooxygenase (alkB), were identified. The predicted functions of these genes indicate that alkane metabolism, sugar synthesis, and the addition of acyl groups are important for the biosynthesis of STLs. Based on these results, we propose a biosynthesis pathway for STLs from alkanes inRhodococcussp. strain SD-74. By overexpressingtlsA, we achieved a 2-fold increase in the production of STLs. This study advances our understanding of bacterial glycolipid production inRhodococcusspecies.
Draft Genome Sequence of the Oleaginous Yeast
Cryptococcus albidus
var.
albidus
