Plasmid gene AZOBR_p60126 impacts biosynthesis of lipopolysaccharide II and swarming motility in Azospirillum brasilense Sp245

2020 ◽  
Vol 60 (7) ◽  
pp. 613-623
Author(s):  
Lilia P. Petrova ◽  
Stella S. Yevstigneyeva ◽  
Igor V. Borisov ◽  
Andrei V. Shelud'ko ◽  
Gennady L. Burygin ◽  
...  
2018 ◽  
Vol 64 (2) ◽  
pp. 107-118 ◽  
Author(s):  
Yulia A. Filip’echeva ◽  
Andrei V. Shelud’ko ◽  
Alexei G. Prilipov ◽  
Gennady L. Burygin ◽  
Elizaveta M. Telesheva ◽  
...  

Azospirillum brasilense can swim and swarm owing to the activity of a constitutive polar flagellum (Fla) and inducible lateral flagella (Laf), respectively. Experimental data on the regulation of the Fla and Laf assembly in azospirilla are scarce. Here, the coding sequence (CDS) AZOBR_p1160043 (fabG1) for a putative 3-oxoacyl-[acyl-carrier protein (ACP)] reductase was found essential for the construction of both types of flagella. In an immotile leaky Fla− Laf− fabG1::Omegon-Km mutant, Sp245.1610, defects in flagellation and motility were fully complemented by expressing the CDS AZOBR_p1160043 from plasmid pRK415. When pRK415 with the cloned CDS AZOBR_p1160045 (fliC) for a putative 65.2 kDa Sp245 Fla flagellin was transferred into the Sp245.1610 cells, the bacteria also became able to assemble a motile single flagellum. Some cells, however, had unusual swimming behavior, probably because of the side location of the organelle. Although the assembly of Laf was not restored in Sp245.1610 (pRK415-p1160045), this strain was somewhat capable of swarming motility. We propose that the putative 3-oxoacyl-[ACP] reductase encoded by the CDS AZOBR_p1160043 plays a role in correct flagellar location in the cell envelope and (or) in flagellar modification(s), which are also required for the inducible construction of Laf and for proper swimming and swarming motility of A. brasilense Sp245.


2020 ◽  
Vol 65 (6) ◽  
pp. 963-972
Author(s):  
Lilia P. Petrova ◽  
Stella S. Yevstigneyeva ◽  
Yulia A. Filip’echeva ◽  
Andrei V. Shelud’ko ◽  
Gennady L. Burygin ◽  
...  

2021 ◽  
Vol 90 (1) ◽  
pp. 64-75
Author(s):  
А. В. Шелудько ◽  
Д. И. Мокеев ◽  
С. С. Евстигнеева ◽  
Ю. А. Филипьечева ◽  
А. М. Буров ◽  
...  

2009 ◽  
Vol 295 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Monica Gupta ◽  
Kestur Krishnamurthy Rao

2018 ◽  
Vol 31 (1) ◽  
pp. 1-8
Author(s):  
MARCOS DA SILVA BRUM ◽  
THOMAS NEWTON MARTIN ◽  
VINÍCIUS SANTOS DA CUNHA ◽  
LUIZ FERNANDO TELEKEN GRANDO ◽  
ALEX TAGLIAPIETRA SCHONELL

ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.


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