Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells

2021 ◽  
Author(s):  
Chiaki Morimoto ◽  
Masahide Takedachi ◽  
Kohsuke Kawasaki ◽  
Junpei Shimomura ◽  
Mari Murata ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Gingival Fibroblasts

scholarly journals The Cytolethal Distending Toxin Induces Receptor Activator of NF-κB Ligand Expression in Human Gingival Fibroblasts and Periodontal Ligament Cells

2005 ◽  
Vol 73 (1) ◽  
pp. 342-351 ◽  
Author(s):  
G. N. Belibasakis ◽  
A. Johansson ◽  
Y. Wang ◽  
C. Chen ◽  
S. Kalfas ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Mrna Expression ◽  
Periodontal Ligament ◽  
Aggressive Periodontitis ◽  
Periodontal Ligament Cells ◽  
Cytolethal Distending Toxin ◽  
Gingival Fibroblasts ◽  
Wild Type ◽  
Rankl Expression ◽  
Receptor Activator

ABSTRACT Actinobacillus actinomycetemcomitans is associated with localized aggressive periodontitis, a disease characterized by rapid loss of the alveolar bone surrounding the teeth. Receptor activator of NF-κB Ligand (RANKL) and osteoprotegerin (OPG) are two molecules that regulate osteoclast formation and bone resorption. RANKL induces osteoclast differentiation and activation, whereas OPG blocks this process by acting as a decoy receptor for RANKL. The purpose of this study was to investigate the effect of A. actinomycetemcomitans on the expression of RANKL and OPG in human gingival fibroblasts and periodontal ligament cells. RANKL mRNA expression was induced in both cell types challenged by A. actinomycetemcomitans extract, whereas OPG mRNA expression remained unaffected. Cell surface RANKL protein was also induced by A. actinomycetemcomitans, whereas there was no change in OPG protein secretion. A cytolethal distending toxin (Cdt) gene-knockout strain of A. actinomycetemcomitans did not induce RANKL expression, in contrast to its wild-type strain. Purified Cdt from Haemophilus ducreyi alone, or in combination with extract from the A. actinomycetemcomitans cdt mutant strain, induced RANKL expression. Pretreatment of A. actinomycetemcomitans wild-type extract with Cdt antiserum abolished RANKL expression. In conclusion, A. actinomycetemcomitans induces RANKL expression in periodontal connective tissue cells. Cdt is crucial for this induction and may therefore be involved in the pathological bone resorption during the process of localized aggressive periodontitis.

Efficient gene transfer to periodontal ligament cells and human gingival fibroblasts by adeno-associated virus vectors

2009 ◽  
Vol 37 (7) ◽  
pp. 502-508 ◽  
Author(s):  
Melanie Kunze ◽  
Anke Huber ◽  
Anna Krajewski ◽  
Esther Lowden ◽  
Natascha Schuhmann ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Periodontal Ligament ◽  
Human Gingival Fibroblasts ◽  
Periodontal Ligament Cells ◽  
Gingival Fibroblasts ◽  
Adeno Associated Virus ◽  
Virus Vectors ◽  
Efficient Gene

scholarly journals Induction of IL-6 and IL-8 Expression by Leptin Treatment in Periodontal Ligament Cells and Gingival Fibroblasts

2013 ◽  
Vol 38 (2) ◽  
pp. 73-80 ◽  
Author(s):  
Hong-Gyu Park ◽  
◽  
Ji-Hye Kim ◽  
Jeong-Heon Cha ◽  
Eun-Jung Bak ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Gingival Fibroblasts
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