Determination of Norfloxacin Free Interstitial Levels in Skeletal Muscle by Microdialysis

2002 ◽  
Vol 91 (11) ◽  
pp. 2433-2440 ◽  
Author(s):  
Rodrigo J. Freddo ◽  
Teresa Dalla Costa
Keyword(s):  
Skeletal Muscle ◽  
Free Interstitial

Antecubital Vein Cannula Position Impacts Assessment of Forearm Glucose Uptake During an Oral Glucose Challenge in Healthy Volunteers

2020 ◽  
Author(s):  
Cécile Bétry ◽  
Aline V. Nixon ◽  
Paul L. Greenhaff ◽  
Elizabeth J. Simpson
Keyword(s):  
Skeletal Muscle ◽  
Blood Glucose ◽  
Glucose Uptake ◽  
Venous Blood ◽  
Whole Body ◽  
Oral Glucose ◽  
Antecubital Vein ◽  
Glucose Challenge ◽  
The Impact

Abstract Introduction Skeletal muscle is a major site for whole-body glucose disposal, and determination of skeletal muscle glucose uptake is an important metabolic measurement, particularly in research focussed on interventions that impact muscle insulin sensitivity. Calculating arterial-venous difference in blood glucose can be used as an indirect measure for assessing glucose uptake. However, the possibility of multiple tissues contributing to the composition of venous blood, and the differential in glucose uptake kinetics between tissue types, suggests that sampling from different vein sites could influence the estimation of glucose uptake. This study aimed to determine the impact of venous cannula position on calculated forearm glucose uptake following an oral glucose challenge in resting and post-exercise states. Materials and Methods In 9 young, lean, males, the impact of sampling blood from two antecubital vein positions; the perforating vein (‘perforating’ visit) and, at the bifurcation of superficial and perforating veins (‘bifurcation’ visit), was assessed. Brachial artery blood flow and arterialised-venous and venous blood glucose concentrations were measured in 3 physiological states; resting-fasted, resting-fed, and fed following intermittent forearm muscle contraction (fed-exercise). Results Following glucose ingestion, forearm glucose uptake area under the curve was greater for the ‘perforating’ than for the ‘bifurcation’ visit in the resting-fed (5.92±1.56 vs. 3.69±1.35 mmol/60 min, P<0.01) and fed-exercise (17.38±7.73 vs. 11.40±7.31 mmol/75 min, P<0.05) states. Discussion Antecubital vein cannula position impacts calculated postprandial forearm glucose uptake. These findings have implications for longitudinal intervention studies where serial determination of forearm glucose uptake is required.

Determination of the Ca2+ and Mg2+ affinity constants of troponin C from eel skeletal muscle and positioning of the single tryptophan in the primary structure

1993 ◽  
Vol 14 (6) ◽  
pp. 585-593 ◽  
Author(s):  
Jean-Marie Fran�ois ◽  
Charles Gerday ◽  
Franklyn G. Prendergast ◽  
James D. Potter
Keyword(s):  
Skeletal Muscle ◽  
Primary Structure ◽  
Troponin C ◽  
Affinity Constants

Chymotryptic Subfragments of Troponin T from Rabbit Skeletal Muscle. I. Determination of the Primary Structure1

1982 ◽  
Vol 91 (4) ◽  
pp. 1257-1265 ◽  
Author(s):  
Masaru TANOKURA ◽  
Yuriko TAWADA ◽  
Iwao OHTSUKI
Keyword(s):  
Skeletal Muscle ◽  
Troponin T ◽  
Rabbit Skeletal Muscle

Administration of a Mixture of Fungal Glucosidases to a Patient with Type II Glycogenosis (Pompe's Disease)

PEDIATRICS ◽  
1968 ◽  
Vol 42 (4) ◽  
pp. 672-676
Author(s):  
Ronald M. Lauer ◽  
Thelma Mascarinas ◽  
Antonio S. Racela ◽  
Antoni M. Diehl ◽  
Barbara Illingworth Brown
Keyword(s):  
Skeletal Muscle ◽  
Enzyme Assay ◽  
Glycogen Content ◽  
Hydrolytic Enzymes ◽  
Type Ii ◽  
Electron Microscopic ◽  
Pompe’S Disease ◽  
Pompe's Disease ◽  
Type Ii Glycogenosis

A case of Type II glycogenosis (Pompe's disease) has been studied by histochemical, electron microscopic, and biochemical techniques. These studies have been made prior to and after the intramuscular administration for 1 week of a mixture of hydrolytic enzymes containing both α-1,4- and α-1,6-glucosidase activities. Electron photomicrographs of the liver before enzyme administration showed glycogen to be located both within and outside of membrane-limited vacuoles. No change in this distribution could be detected in tissue removed by biopsy after enzyme administration. This impression was confirmed by the determination of glycogen content which was shown to be unchanged. Nevertheless, the liver was found by enzyme assay to contain the administered enzyme. Leucocytes isolated from blood taken 4 hours after the last enzyme injection were also shown to contain the parenterally administered glucosidases. In skeletal muscle glycogen was present chiefly as extrasaccular deposits which were unchanged in appearance by enzyme administration. No glucosidase activity was demonstrable in the skeletal muscle after such a treatment. Myocardium sectioned after autopsy had major deposits of glycogen in extrasaccular areas.

Determination of Skeletal Muscle Microvascular Flowmotion with Contrast-Enhanced Ultrasound

2017 ◽  
Vol 43 (9) ◽  
pp. 2013-2023 ◽  
Author(s):  
Sarah J. Blackwood ◽  
Renee M. Dwyer ◽  
Eloise A. Bradley ◽  
Michelle A. Keske ◽  
Stephen M. Richards ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Contrast Enhanced Ultrasound ◽  
Contrast Enhanced

Determination of the Magnitude of the Water-Exclusive Pathway in Cat Skeletal Muscle Microvasculature

1996 ◽  
Vol 3 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Matthew B. Wolf
Keyword(s):  
Skeletal Muscle

Effects of Co60 Irradiation on Muscle Fiber Types of the Grass Frog

2000 ◽  
Vol 6 (S2) ◽  
pp. 852-853
Author(s):  
Glenn M. Cohen ◽  
Margaret F. Scott
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Striated Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Mitochondrial Enzymes ◽  
Metabolic Characteristics ◽  
Long Latency ◽  
Low Levels

Striated skeletal muscle has been considered radioresistant because it is highly differentiated and post-mitotic. Striated muscle does, however, respond to irradiation with morphological and biochemical changes after short and long latency periods; vascular and/or neurological impairments might contribute to the delayed responses to irradiation.The objective of the present study was to determine the susceptibility of three amphibian muscle fiber types to Co60 irradiation. In amphibians, the three major fiber types are 1) large twitch fibers, which contain low levels of mitochondrial enzymes and lipids, but intermediate levels of glycogen; 2) small twitch fibers, which contain high levels of both glycolytic and mitochondrial enzymes (FIG. 1); and tonic fibers, which contain low levels of all three histochemical markers. Thus, the determination of susceptibility of different amphibian fiber types to irradiation might indicate whether the metabolic characteristics of the fibers, rather than morphological or electrical properties, could serve as an early indicator of radiation damage.

scholarly journals Isolation of human skeletal muscle myosin heavy chain and actin for measurement of fractional synthesis rates

1998 ◽  
Vol 275 (6) ◽  
pp. E1092-E1099 ◽  
Author(s):  
D. L. Hasten ◽  
G. S. Morris ◽  
S. Ramanadham ◽  
K. E. Yarasheski
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Sodium Dodecyl ◽  
Synthesis Rate ◽  
Simple Method ◽  
Skeletal Muscle Myosin ◽  
Fractional Synthesis

Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), we have developed a simple method to isolate myosin heavy chain (MHC) and actin from small (60–80 mg) human skeletal muscle samples for the determination of their fractional synthesis rates. The amounts of MHC and actin isolated are adequate for the quantification of [13C]leucine abundance by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Fractional synthesis rates of mixed muscle protein (MMP), MHC, and actin were determined in six healthy young subjects (27 ± 1 yr) after they received a 14-h intravenous infusion (prime = 7.58 μmol/kg body wt, constant infusion = 7.58 μmol ⋅ kg body wt−1 ⋅ h−1) of [1-13C]leucine. The fractional synthesis rates of MMP, MHC, and actin were found to be 0.0468 ± 0.0048, 0.0376 ± 0.0033, and 0.0754 ± 0.0078%/h, respectively. Overall, the synthesis rate of MHC was 20% lower ( P = 0.012), and the synthesis rate of actin was 61% higher ( P = 0.060, not significant) than the MMP synthesis rate. The isolation of these proteins for isotope abundance analysis by GC-C-IRMS provides important information about the synthesis rates of these specific contractile proteins, as opposed to the more general information provided by the determination of MMP synthesis rates.

scholarly journals Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo

1980 ◽  
Vol 188 (3) ◽  
pp. 705-713 ◽  
Author(s):  
G Livesey ◽  
P Lund
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Bacillus Subtilis ◽  
Branched Chain Amino Acids ◽  
Rat Tissues ◽  
Arterial Blood ◽  
Branched Chain ◽  
Arteriovenous Difference

1. A procedure is described for the purification of leucine dehydrogenase (EC 1.4.1.9) from Bacillus subtilis. 2. The preparation is suitable for the quantitative assay of branched-chain amino acids and their 2-oxoacid analogues. 3. The content of total branched-chain 2-oxoacids in freeze-clamped liver, kidney, heart or mammary gland of fed rats is less than 5 nmol/g fresh wt. Higher amounts are present in skeletal muscle and arterial blood (25 +/- 4 nmol per g fresh wt., and 33 +/- 6 nmol per ml respectively; means +/- S.D. of 3 and 11 animals respectively). The values are not significantly affected by starvation for 24 h. 4. Arteriovenous difference measurements show that considerable amounts of branched-chain 2-oxoacids are released by skeletal muscle into the circulation and similar amounts are removed by the liver (about 1 mmol/24 h in a 400 g rat).

Meat Tenderness Factors, Determination of Mucoprotein in Skeletal Muscle

1961 ◽  
Vol 9 (6) ◽  
pp. 421-424 ◽  
Author(s):  
E. N. McIntosh
Keyword(s):  
Skeletal Muscle ◽  
Meat Tenderness
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