A Novel Approach for Quantitation of Nonderivatized Sialic Acid in Protein Therapeutics Using Hydrophilic Interaction Chromatographic Separation and Nano Quantity Analyte Detection

2015 ◽  
Vol 104 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Letha Chemmalil ◽  
Sreekanth Suravajjala ◽  
Kate See ◽  
Eric Jordan ◽  
Marsha Furtado ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Chromatographic Separation ◽  
Protein Therapeutics ◽  
Hydrophilic Interaction ◽  
Novel Approach ◽  
Analyte Detection

LC-MS in combination with DMBA derivatization for sialic acid speciation and distribution analysis in fish tissues

2020 ◽  
Vol 12 (17) ◽  
pp. 2221-2227 ◽  
Author(s):  
Junjie Du ◽  
Qiwei Zhang ◽  
Jianjun Li ◽  
Qi Zheng
Keyword(s):  
Sialic Acid ◽  
Chromatographic Separation ◽  
Separation Efficiency ◽  
Speciation Analysis ◽  
Distribution Analysis ◽  
Fish Tissues

For sialic acid speciation analysis, DMBA provides superior chromatographic separation efficiency and comparable MS/MS spectra with DMB.

scholarly journals S95 Exploiting the immunoregulatory role of Siglec-E via sialic acid-functionalised nanoparticles as a novel approach for the treatment of acute lung injury

Thorax ◽  
2013 ◽  
Vol 68 (Suppl 3) ◽  
pp. A51.1-A51 ◽  
Author(s):  
MK Greene ◽  
S Spence ◽  
F Fay ◽  
D Small ◽  
D Schmid ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Sialic Acid ◽  
Lung Injury ◽  
Novel Approach

O.7 Novel approach to sialic acid therapy in DMRV/hIBM mouse model

2010 ◽  
Vol 20 (9-10) ◽  
pp. 636
Author(s):  
M.C. Malicdan ◽  
S. Noguchi ◽  
K. Momma ◽  
F. Funato ◽  
Y.K. Hayashi ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Mouse Model ◽  
Acid Therapy ◽  
Novel Approach

Zwitterion Chromatography-Hydrophilic Interaction Chromatography for Separation and Quantitative of Rutin and Quercetin from Herbs and Bee Products

2021 ◽  
Vol 43 (4) ◽  
pp. 484-484
Author(s):  
Raad Radi Karabat Raad Radi Karabat ◽  
Ashraf Saad Rasheed and Mohammed Jasim M Hassan Ashraf Saad Rasheed and Mohammed Jasim M Hassan
Keyword(s):  
Cancer Cells ◽  
Chromatographic Separation ◽  
Human Cancer ◽  
Stationary Phases ◽  
Hydrophilic Interaction Chromatography ◽  
Hydrophilic Interaction ◽  
Experimental Animals ◽  
Cancer Induction ◽  
Rutin And Quercetin

Quercetin and rutin have a number of useful pharmacological consequences and a possible medication of the future. The growth of cancer cells was observed in vitro and the production of tumors in experimental animals was decreased. In addition to preventing human cancer induction and progression. A set of four HILIC stationary phases, two home-made columns with different space chains between the ionic site groups, and two commercially stationary phases HALOand#174;HILIC 2.7 and ZICand#174;–pHILIC for chromatographic separation and quantitation of quercetin and rutin in herbs and bee products. The separation mechanisms are based on hydrophobic and hydrophilic for the quercetin and rutin, respectively. The validated method was successfully applied in bee products and herbs. The results showed that the HILIC methods were simple and reliable and could be used in bee products and herbs to detect the content of quercetin and rutin.

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