Development of novel species-specific primers for species identification of the Lactobacillus casei group based on RAPD fingerprints

2009 ◽  
Vol 89 (11) ◽  
pp. 1831-1837 ◽  
Author(s):  
Chien-Hsun Huang ◽  
Fwu-Ling Lee
Keyword(s):  
Species Identification ◽  
Lactobacillus Casei ◽  
Novel Species ◽  
Specific Primers ◽  
Rapd Fingerprints ◽  
Species Specific ◽  
Lactobacillus Casei Group

Agronomically important thrips: development of species-specific primers in multiplex PCR and microarray assay using internal transcribed spacer 1 (ITS1) sequences for identification

2014 ◽  
Vol 105 (1) ◽  
pp. 52-59 ◽  
Author(s):  
W.B. Yeh ◽  
M.J. Tseng ◽  
N.T. Chang ◽  
S.Y. Wu ◽  
Y.S. Tsai
Keyword(s):  
Multiplex Pcr ◽  
Species Identification ◽  
Internal Transcribed Spacer ◽  
High Throughput Screening ◽  
Virus Transmission ◽  
Specific Primers ◽  
Its1 Sequence ◽  
Microarray Assay ◽  
Thrips Species ◽  
Species Specific

AbstractThrips, the sole vector of plantTospovirus, are major pests of many agricultural crops throughout the world. Molecular approaches have been applied in recent decades to identify these minute and morphologically difficult to distinguish insects. In this study, sequences of internal transcribed spacer 1 (ITS1) region of 15 agronomically important thrips, including several virus transmission species, have been analyzed in order to design species-specific primers for multiplex PCR and probes for microarray assay. That the ITS1 sequence distances within species were smaller than those among species suggests that the ITS1 fragment can be used for thrips species identification. The specificity and stability of these primers, combined with universal paired primers, were tested and verified in multiplex PCR. Using these specific primers as probes, microarray assay showed that PCR products of all thrips species hybridized consistently to their corresponding probes, though some signals were weak. We have demonstrated that multiplex PCR using specific primers based on ITS1 sequences is a simple, reliable, and cost-effective diagnostic tool for thrips species identification. Moreover, the DNA microarray assay is expected to extend into a reliable high-throughput screening tool for the vast numbers of thrips.

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