Clinical value of in vitro drug sensitivity testing based on short-term effects on dna and rna metabolism in ovarian cancer

1989 ◽  
Vol 41 (3) ◽  
pp. 201-205 ◽  
Author(s):  
S. K. Khoo ◽  
T. Hurst ◽  
M. J. Webb ◽  
G. Dickie ◽  
J. Kearsley ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Drug Sensitivity ◽  
Rna Metabolism ◽  
Short Term ◽  
Clinical Value ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing ◽  
Dna And Rna ◽  
Short Term Effects

Short-term organoid culture for drug sensitivity testing in high-grade serous ovarian cancer

2019 ◽  
Vol 153 (3) ◽  
pp. e13 ◽  
Author(s):  
K. Gotimer ◽  
H. Chen ◽  
G.S. Leiserowitz ◽  
L.H. Smith
Keyword(s):  
Ovarian Cancer ◽  
Drug Sensitivity ◽  
High Grade ◽  
Serous Ovarian Cancer ◽  
Short Term ◽  
Sensitivity Testing ◽  
Organoid Culture ◽  
Drug Sensitivity Testing

Short-term organoid culture for drug sensitivity testing in high-grade serous ovarian cancer

2019 ◽  
Vol 154 ◽  
pp. 92-93 ◽  
Author(s):  
K. Gotimer ◽  
H. Chen ◽  
G.S. Leiserowitz ◽  
L.H. Smith
Keyword(s):  
Ovarian Cancer ◽  
Drug Sensitivity ◽  
High Grade ◽  
Serous Ovarian Cancer ◽  
Short Term ◽  
Sensitivity Testing ◽  
Organoid Culture ◽  
Drug Sensitivity Testing

Short-term Organoid Culture For Drug Sensitivity Testing in High Grade Serous Ovarian Cancer

2020 ◽  
Vol 156 (3) ◽  
pp. e27 ◽  
Author(s):  
Kristin Gotimer ◽  
Hui Chen ◽  
Clifford Tepper ◽  
Anthony Karnezis ◽  
Jeremy Chien ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Drug Sensitivity ◽  
High Grade ◽  
Serous Ovarian Cancer ◽  
Short Term ◽  
Sensitivity Testing ◽  
Organoid Culture ◽  
Drug Sensitivity Testing

In vitro Anticancer Drug Sensitivity Testing

2015 ◽  
pp. 13-14
Author(s):  
Ya. V. Dobrynin ◽  
G. V. Vyshinskaya
Keyword(s):  
Anticancer Drug ◽  
Drug Sensitivity ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing

Correlation of in vitro drug sensitivity testing results with response to chemotherapy and survival: Comparison of non-small cell lung cancer and small cell lung cancer

1996 ◽  
Vol 63 (S24) ◽  
pp. 173-185 ◽  
Author(s):  
Gail L. Shaw ◽  
Adi F. Gazdar ◽  
Ruby Phelps ◽  
Seth M. Steinberg ◽  
R. Iiona Linnoila ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Drug Sensitivity ◽  
Small Cell ◽  
Small Cell Lung ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing ◽  
Response To Chemotherapy

Stroma-free long-term growth of primary acute myeloid leukemia (AML) cells.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e18535-e18535
Author(s):  
Jing Chen ◽  
Glorymar Ibanez Sanchez ◽  
Paul Calder ◽  
Mark G. Frattini
Keyword(s):  
Growth Factors ◽  
Conditioned Medium ◽  
Stromal Cells ◽  
Drug Sensitivity ◽  
Growth Conditions ◽  
Leukemic Cells ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing

e18535 Background: To individualize therapy for relapsed/refractory AML patients, optimal in-vitro culture conditions to support primary leukemic cells are essential for drug sensitivity testing. Our lab has validated a high throughput chemosensitivity assay with primary AML cells maintained by growth factors (cytokines); however, growth factors have not been shown to support long-term assays of primary AML cells. Stromal cells of the tumor microenvironment are crucial to maintain normal hematopoiesis and leukemic cells and have been shown to support long term in-vitro expansion of primary AML cells. However, there is little information characterizing these growth conditions. The aim of this study was to compare long-term proliferation and phenotypes of primary AML cells with growth factors or stromal support to best determine their utility as a platform for drug sensitivity testing in functional assays. Methods: Patient-derived AML cells were cultured in 96-well plates in: 1) cell culture medium only 2) Human HS5 or HS27 stromal cells 3) HS5 or HS27 stroma-conditioned media or 4) cytokine cocktail. Viability readout by Guava ViaCount and leukemic cell surface phenotypes by fluorescently-conjugated antibodies were performed weekly over 3 weeks. Results: Primary AML cells cultured with only cytokines maintained proliferation at 3 weeks. In comparison, AML cells cultured in HS5 stroma-conditioned medium also maintained proliferation at a similar rate at 3 weeks, while co-culture with HS5 stromal cells demonstrated significantly higher proliferation. Leukemic immunophenotypes were maintained for all growth conditions over 3 weeks. Conclusions: Contrary to known data, primary AML cells with cytokines continued to expand at 3 weeks, at a similar rate to HS5 stroma-conditioned medium, a finding that has not been reported. Consistent with previous studies, we confirmed that stromal cells such as HS5 can provide long-term in-vitro expansion of primary AML cells, which cannot be substituted by stroma-conditioned medium. The ability to maintain long-term expansion of primary AML cells by both cytokines and stromal cells sets up a platform for a direct comparison of high throughput drug sensitivity testing under these growth conditions.

Blastocystis hominis: neutral red supravital staining and its application to in vitro drug sensitivity testing

2000 ◽  
Vol 86 (7) ◽  
pp. 573-581 ◽  
Author(s):  
Alexandre A. Vdovenko ◽  
John E. Williams
Keyword(s):  
Drug Sensitivity ◽  
Neutral Red ◽  
Blastocystis Hominis ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing ◽  
Supravital Staining

Prognostic Relevance of In-Vitro Drug Sensitivity Testing in Adult AML

2001 ◽  
pp. 251-256 ◽  
Author(s):  
P. Staib ◽  
T. SchinköThe ◽  
S. Wiedenmann ◽  
T. Dimski ◽  
C. Schoch ◽  
...  
Keyword(s):  
Drug Sensitivity ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing ◽  
Prognostic Relevance

An in Vitro Micromethod for Drug Sensitivity Testing of Leishmania

1989 ◽  
Vol 41 (3) ◽  
pp. 318-330 ◽  
Author(s):  
Joan E. Jackson ◽  
John D. Tally ◽  
Douglas B. Tang
Keyword(s):  
Drug Sensitivity ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing
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