A consecutive preparation method based upon accelerated solvent extraction and high-speed counter-current chromatography for isolation of aesculin from Cortex fraxinus

2012 ◽  
Vol 35 (24) ◽  
pp. 3609-3614 ◽  
Author(s):  
Xing Tong ◽  
Ting Zhou ◽  
Xiaohua Xiao ◽  
Gongke Li
Keyword(s):  
Solvent Extraction ◽  
High Speed ◽  
Preparation Method ◽  
Accelerated Solvent Extraction ◽  
Counter Current

Antioxidant Activities of Flavonoids from Canavalia maritime

2013 ◽  
Vol 834-836 ◽  
pp. 577-581
Author(s):  
Jian Chao Deng ◽  
Gai Gai Niu ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Yong Chuan Deng ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Hydroxyl Radical ◽  
High Speed ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Counter Current

Flavonoids was isolated from canavalia maritime by accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC). The antioxidant activities of flavonoids were investigated in vitro and evaluated by IC50. The results indicated that flavonoids possessed obvious reducing power and significant inhibitory effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggest that canavalia maritime flavonoids could be a suitable natural antioxidant for humans.

Separation and preparation of two rotenoids from the roots of Derris by high-speed counter-current chromatography

2010 ◽  
Vol 28 (8) ◽  
pp. 813-816
Author(s):  
Wei JI ◽  
Yulan CUI ◽  
Renjuan CHEN ◽  
Xinren LIN ◽  
Qingxin ZHOU ◽  
...  
Keyword(s):  
High Speed ◽  
Counter Current

Isolation and purification of flavones from Murraya exotica L. by high-speed counter-current chromatography

2010 ◽  
Vol 28 (4) ◽  
pp. 383-387 ◽  
Author(s):  
Aiyi PENG ◽  
Xuewei QU ◽  
Hui LI ◽  
Lu GAO ◽  
Bo YU ◽  
...  
Keyword(s):  
High Speed ◽  
Isolation And Purification ◽  
Murraya Exotica ◽  
Counter Current

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent

2020 ◽  
Author(s):  
Xue Yang ◽  
Yongling Liu ◽  
Tao Chen ◽  
Nana Wang ◽  
Hongmei Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Dimethyl Sulfoxide ◽  
Efficient Method ◽  
Crude Extract ◽  
High Speed ◽  
Glacial Acetic Acid ◽  
Butyl Alcohol ◽  
Preparative Hplc ◽  
Counter Current

Abstract Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.

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