Application of accelerated solvent extraction coupled with high-performance counter-current chromatography to extraction and online isolation of chemical constituents from Hypericum perforatum L.

2011 ◽  
Vol 1218 (20) ◽  
pp. 2827-2834 ◽  
Author(s):  
Yuchi Zhang ◽  
Chunming Liu ◽  
Min Yu ◽  
Zhengkun Zhang ◽  
Yanjuan Qi ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Chemical Constituents ◽  
Accelerated Solvent Extraction ◽  
Performance Counter ◽  
Hypericum Perforatum L ◽  
Counter Current

scholarly journals Effects of circadian clock and light on melatonin concentration in Hypericum perforatum L. (St. John’s Wort)

2020 ◽  
Vol 61 (1) ◽  
Author(s):  
Ming-Hsiu Chung ◽  
Tzu-Shing Deng
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Fresh Weight ◽  
Melatonin Concentration ◽  
Long Day ◽  
Spatiotemporal Regulation ◽  
Circadian Time ◽  
Hypericum Perforatum L ◽  
Pharmaceutical Industries ◽  
Oriented Research

Abstract Background Melatonin acts as a signaling hormone and entraining agent in many organisms. We studied the spatiotemporal regulation and influence of light (photoperiods, intensities, and spectral qualities) on melatonin concentration in the medicinal herb Hypericum perforatum L. Furthermore, melatonin concentrations in the leaves of eight species of the Hypericum genus were compared and analyzed using high-performance liquid chromatography. Results Melatonin concentration was found to be the highest in its flowers and leaves. The leaves exhibited a rhythmic variation in melatonin concentration of approximately 24 h under both light–dark entrained (Zeitgeber time) and constant light [circadian time (CT)] conditions, with melatonin concentration peaking at approximately CT6 in the middle of the subjective day. Melatonin concentration was influenced significantly by not only photoperiods but also applied light’s wavelength and intensity. It was approximately six times higher under long-day conditions (18-h light:6-h dark) than under short-day photoperiods (10-h light:14-h dark) and was the highest (131 μg/g fresh weight [FW]) under treatment with blue light at an intensity of 45 µmol·m2/s of photons. The melatonin concentration of the two examined Hypericum spp., namely H. kouytchense Lev. and H. coris L., were approximately twice that of H. perforatum L. Conclusion Our findings provide first insights on melatonin-related functions and mechanisms in the circadian system of H. perforatum and useful resources for further melatonin-oriented research and possible applications in agriculture and pharmaceutical industries.

Study on Gasoline Combustion Dust with ASE and HPLC

2013 ◽  
Vol 750-752 ◽  
pp. 1852-1854
Author(s):  
Shu Jun Liu ◽  
Kun Feng ◽  
Hao Nan Xu ◽  
Ying Wang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Extraction Method ◽  
High Performance ◽  
Accelerated Solvent Extraction ◽  
Extraction Time ◽  
Efficient Extraction ◽  
Optimum Extraction

The gasoline combustion dusts was analyzed by accelerated solvent extraction-high performance liquid chromatography(ASE-HPLC) in this paper. The optimum extraction conditions were that extraction time was 30min, temperature was 120°C and the pressure was 100Mpa. It is a efficient extraction method for a laboratory to quickly extract gasoline combustion dust samples.

scholarly journals Development of an Efficient Protocol for Cimifugin Isolation from Peucedanum schottii and Evaluation of Enzyme Inhibitory Activity

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100
Author(s):  
Ewelina Kozioł ◽  
Ilkay Erdogan Orhan ◽  
F. Sezer Senol ◽  
Kalina Alipieva ◽  
Milen Georgiev ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
High Performance ◽  
Performance Counter ◽  
Inhibitory Potential ◽  
Counter Current ◽  
First Time ◽  
Analytical Scale

The dichloromethane (DCM) extract of the fruits of Peucedanum schottii Besser ex DC. (Apiaceae) was subjected to high-performance counter-current chromatography (HPCCC) for the efficient and fast separation (30 min) and isolation of cimifugin using an ethyl acetate: water (1:1 v/v, K = 1.01) system. The analytical scale-optimized separation was easily scaled to semi-preparative conditions. Cimifugin (11.25% yield, 96.5% purity) was isolated for the first time from P. schottii and characterized by NMR spectroscopy. Cimifugin and the crude DCM extract were evaluated using ELISA microtiter assays for their inhibitory potential against the cholinesterases (acetylcholinesterase - AChE and butyrylcholinesterase - BChE), and tyrosinase (TYR), which are key enzymes for the treatment of some neurodegenerative diseases, i.e. Alzheimer's and Parkinson's. The crude extract exhibited a weak inhibitory activity against AChE, BChE, and TYR (4.2, 35.5, and 0% at 100 μg mL−1 and 10.3, 40.0, and 12.2% at 200 μg mL−1, respectively), while cimifugin displayed low to moderate inhibition towards AChE and BChE (3.1 and 21.6%, respectively) at 200 μg mL−1.

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