Determination of neurotransmitter amino acids in mouse central nervous system by CE-LIF

2013 ◽  
Vol 36 (19) ◽  
pp. 3264-3269 ◽  
Author(s):  
Xu-Jie Xiong ◽  
Xiao-Feng Guo ◽  
Xu-Xia Ge ◽  
Hong Wang ◽  
Hua-Shan Zhang
Keyword(s):  
Amino Acids ◽  
Central Nervous System ◽  
Nervous System ◽  
Neurotransmitter Amino Acids ◽  
Mouse Central Nervous System

scholarly journals CB1 and LPA1 Receptors Relationship in the Mouse Central Nervous System

2019 ◽  
Vol 12 ◽  
Author(s):  
Estíbaliz González de San Román ◽  
Iván Manuel ◽  
Catherine Ledent ◽  
Jerold Chun ◽  
Fernando Rodríguez de Fonseca ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Mouse Central Nervous System

scholarly journals Apoptotic Cells, Including Macrophages, Are Prominent in Theiler's Virus-Induced Inflammatory, Demyelinating Lesions

2003 ◽  
Vol 77 (7) ◽  
pp. 4383-4388 ◽  
Author(s):  
Brian P. Schlitt ◽  
Matthew Felrice ◽  
Mary Lou Jelachich ◽  
Howard L. Lipton
Keyword(s):  
Central Nervous System ◽  
T Cells ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Apoptotic Cells ◽  
Theiler's Virus ◽  
Demyelinating Lesions ◽  
Encephalomyelitis Virus ◽  
Mouse Central Nervous System

ABSTRACT Theiler's murine encephalomyelitis virus (TMEV) persists in the mouse central nervous system principally in macrophages, and infected macrophages in culture undergo apoptosis. We have detected abundant apoptotic cells in perivascular cuffs and inflammatory, demyelinating lesions of SJL mice chronically infected with TMEV. T cells comprised 74% of apoptotic cells, while 8% were macrophages, 0.6% were astrocytes, and ∼17% remained unidentified. In situ hybridization revealed viral RNA in ∼1% of apoptotic cells.

scholarly journals The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies

2021 ◽  
Vol 4 (1) ◽  
pp. 11
Author(s):  
Evrysthenis Vartholomatos ◽  
George Vartholomatos ◽  
George A. Alexiou ◽  
Georgios S. Markopoulos
Keyword(s):  
Central Nervous System ◽  
Flow Cytometry ◽  
Nervous System ◽  
Therapeutic Agents ◽  
Phenotypic Characterization ◽  
Cell Phenotype ◽  
Analytical Technique ◽  
The Past ◽  
Current Review

Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as by the acquired chemoresistance of CNSM cells. Flow Cytometry is an analytical technique with the ability to quantify cell phenotype and to categorize cell populations on the basis of their characteristics. In the current review, we summarize the Flow Cytometry methodologies that have been used to study different phenotypic aspects of CNSMs. These include DNA content analysis for the determination of malignancy status and phenotypic characterization, as well as the methodologies used during the development of novel therapeutic agents. We conclude with the historical and current utility of Flow Cytometry in the field, and we propose how we can exploit current and possible future methodologies in the battle against this dreadful type of malignancy.

scholarly journals Bioanalysis of small-molecule drugs in nasal and paranasal tissues and secretions: Current status and perspectives

2012 ◽  
Vol 10 (3) ◽  
pp. 686-702
Author(s):  
Ana Serralheiro ◽  
Gilberto Alves ◽  
Amílcar Falcão
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Small Molecule ◽  
Intranasal Administration ◽  
Quantitative Measurement ◽  
Current Status ◽  
Small Molecule Drugs ◽  
Development And Validation ◽  
Liquid Chromatographic

AbstractOver the last years, interest in intranasal administration as an alternative and promising route for the delivery of drugs withlocal, systemic, and even central nervous system action has tremendously increased. Accordingly, understanding of the propertiesand characteristics of the nasal cavity as well as the biodisposition processes of drugs into the nasal compartments is acquiringa significant prominence in the field of pharmacology. In this context, the development and validation of bioanalytical methodologies for the quantitative measurement of drugs and their metabolites in nasal and paranasal tissues and/or secretions is of the utmostimportance. However, currently, information concerning bioanalysis of drugs in nasal and paranasal tissues and/or secretionsis scattered. This review aims to provide a valuable overview of the methodologies that have been used for the collectionand preparation of nasal and paranasal samples with special emphasis placed on the review of liquid chromatographic methodsemployed for the quantitative determination of small-molecule drugs and their metabolites in such specimens.

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