Compression period in a diesel engine is generally seen as initial characteristics before injection into combustion chamber. A free-piston type rapid compression machine (RCM) has been designed simulate the combustion phenomena in order to observe the chemical and physical kinetics studies at elevated pressures and temperatures. Purpose of this study is to clarify the effects of wall cylinder temperature on air heat release, especially during compression period. This method can a light piston is pneumatically shot and gets hammered in stopper at compression end. Measurements were made on a light piston compress in a rapid compression machine (RCM) with intended to simulate the actual compression period related phenomena. During this phenomena, the ambient temperature influences from the cylinder wall temperature has appear beneficial for the biodiesel fuel premixing and achieving best mixture preparation There are three tests will used to investigate the effects of the variant temperature of wall cylinder during compression period. Results show high temperature on cylinder wall will be effects decrease during temperature drop after compression stroke. The new characteristics rapid compression machine (RCM) is to develop and analyse before combustion process.