Column Chromatographic Determination of Unsaponifiable Matter in Fats and Oils

1981 ◽  
Vol 83 (2) ◽  
pp. 73-76 ◽  
Author(s):  
M. K. Kundu ◽  
A. T. Deb
Keyword(s):  
Unsaponifiable Matter ◽  
Fats And Oils ◽  
Chromatographic Determination ◽  
Column Chromatographic

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. II. Collaborative Study

1969 ◽  
Vol 52 (4) ◽  
pp. 778-781
Author(s):  
C W Thorpe
Keyword(s):  
Unsaponifiable Matter ◽  
Collaborative Study ◽  
Fats And Oils ◽  
Gas Liquid Chromatography ◽  
Animal Fats ◽  
Animal Fat ◽  
Low Levels ◽  
Positive Results ◽  
Column Chromatographic

Abstract A method employing TLC-GLC for detecting animal fats in vegetable fats was subjected to collaborative study. The method involves preparative TLC of unsaponifiable matter followed by GLC of the isolated sterols. The results of this study, based on GLC determination of cholesterol in the isolated sterols, indicate that low levels (2.5%) of animal fat can be detected. The possibility of obtaining false positive results is greatly reduced compared with direct GLC of unsaponifiable matter or column chromatographic fractionation of unsaponifiable matter followed by GLC analysis.

Gas-Liquid Chromatographic Determination of Vitamin D2 in Multiple Vitamin Tablets Containing Minerals and Vitamin E Acetate

1974 ◽  
Vol 57 (5) ◽  
pp. 1089-1091
Author(s):  
David O Edlund ◽  
Florido A Filippini ◽  
James K Datson
Keyword(s):  
Vitamin E ◽  
Chromatographic Determination ◽  
Vitamin D2 ◽  
Vitamin E Acetate ◽  
Chromatographic Procedure ◽  
Ether Solution ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Column Chromatographic

Abstract A gas-liquid chromatographic procedure used to determine vitamin D2 in multiple vitamin tablets has been modified to make it applicable for analysis of multiple vitamin tablets containing minerals and vitamin E acetate. The procedure modifications involve pre-extraction with ether, solution in an alcoholic sulfuric acid-pyridine mixture, and column chromatographic separation on phosphate-treated alumina. The modified procedure has been statistically evaluated. A 2.2% coefficient of variation and 100.3% average recovery were obtained for the samples evaluated.

Column Chromatographic Determination of Certifiable Colors in Lipsticks

1963 ◽  
Vol 46 (6) ◽  
pp. 1013-1017
Author(s):  
Rachel Sclar Silk
Keyword(s):  
Binary Mixture ◽  
Ammonium Hydroxide ◽  
Chromatographic Determination ◽  
Aqueous Alcohol ◽  
Partition Chromatography ◽  
Immobile Phase ◽  
Column Chromatographic

Abstract Column partition chromatography on Celite is used to separate the colors present in lipsticks. In the proposed procedure the immobile phase is aqueous alcohol plus ammonium hydroxide. A small portion of lipstick is ground with a portion of Celite containing the aqueous immobile phase and packed on top of a prepared colrate umn. Solvents of increasing polarity are used to elute individual colors except for one binary mixture. D&C Red No. 19 and D&C Red No. 36 and/or D&C Orange No. 17 elute together.

Gas-Liquid Chromatographic Determination of TYans-Unsaturation in Fats and Oils on Packed Columns: Effect of Positional Isomers

1978 ◽  
Vol 61 (3) ◽  
pp. 702-708
Author(s):  
Henry B S Conacher ◽  
Jagannath R Iyengar
Keyword(s):  
Fats And Oils ◽  
Chromatographic Determination ◽  
Packed Columns ◽  
Gas Liquid Chromatography ◽  
Positional Isomers ◽  
Tubular Columns ◽  
Acid Isomerization ◽  
Cis And Trans ◽  
Liquid Chromatographic

Abstract Studies in our laboratories indicated that the trans contents of a number of commercial margarines, determined by gas-liquid chromatography (GLC) on a 15 ft column packed with 10% Silar IOC, were approximately 80-85% of those determined by infrared spectroscopy. This is mainly due to overlap of some of the trans positional isomers with the cis positional isomers in the octadecenoate fraction, shown by studies involving determination of the transoctadecenoate content of the margarines by silver ion thin layer chromatography/GLC, and by preparation and study of the GLC behavior of the cis and trans positional isomers of Δ6-, Δ9-, Δ12-, and Δl5-octadecenoates. The last 3 isomers were prepared by hydrazine reduction and nitrous acid isomerization of linoleic and linolenic acids. Since it is possible that longer, more efficient columns might result in better separation of the cis- and traru-octadecenoate isomers, it is recommended that studies be continued on longer packed columns (20 ft) and possibly on support-coated open tubular columns.

Ion-Pair Column Chromatographic Determination of Trimethobenzamide Hydrochloride in Capsule and Injection Dosage Forms: Collaborative Study

1985 ◽  
Vol 68 (5) ◽  
pp. 1055-1057
Author(s):  
Helen Naviasky
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Dosage Forms ◽  
Ion Pair ◽  
Drug Substance ◽  
Uv Spectrophotometry ◽  
Coefficients Of Variation ◽  
Column Chromatographic

Abstract An ion-pair column chromatographic method was developed for the determination of trimethobenzamide hydrochloride in capsules and injection dosage forms. Detection is by UV spectrophotometry at 261 nm. Recoveries by the Associate Referee ranged from 98.3 to 101.0% for the drug substance. Results by 5 collaborators for capsules averaged 99.1% of labeled or theoretical with coefficients of variation (CVs) of 1.81% (reproducibility) and 1.17% (repeatability); results for injec- , tions averaged 100.4% of labeled or theoretical with CVs of 1.91% (reproducibility) and 0.69% (repeatability). The method has been adopted official first action

Gas and Thin Layer Chromatographic Determination of Sugars in Cocoa Products

1971 ◽  
Vol 54 (4) ◽  
pp. 937-939
Author(s):  
Milton A Luke
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Water Solution ◽  
Fats And Oils ◽  
Chromatographic Determination ◽  
Trifluoroacetic Anhydride ◽  
Cocoa Products ◽  
Identification Test

Abstract A GLC method is presented in which trifluoroacetate derivatives are used for the determination of sucrose and lactose in cocoa products. The samples are extracted with ether-petroleum ether to remove fats and oils, and the sugars are then extracted with water. An aliquot of the water solution is evaporated to dryness; the derivative is formed with trifluoroacetic anhydride and is quantitatively measured and identified by GLC. Recoveries ranged from 90 to 105%. The method is also applicable to glucose and fructose. A TLC method is also described which can be used as an additional identification test and for the semiquantitative measurement of glucose, fructose, sucrose, lactose, and maltose. It is recommended that the GLC and TLC methods for analysis of sugars in cocoa products be studied collaboratively.

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