Dynamic macromolecular composition and high exudation rates in Prochlorococcus

The work is devoted to modeling the growth of optically dense microalgae cultures in natural light. The basic model is based on the idea of the two-stage photoautotrophic growth of microalgae. It is shown that the increase in the intensity of sunlight in the first half of the day can be described by a linear equation. Analytical equations for the growth of biomass of microalgae and its macromolecular components are obtained. As the initial conditions, it is assumed that at the time of sunrise, the concentration of reserve biomass compounds is zero. The simulation results show that after sunrise, the growth of the microalgae culture is due only to an increase in the reserve part of the biomass, while the structural part practically does not change over six hours. Changes in the ratio of the reserve and structural parts of the biomass indicate a change in the biochemical composition of cells.

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Differences in the macromolecular composition of the zona pellucida isolated from pig oocytes, eggs, and zygotes

Journal of Experimental Zoology ◽

10.1002/jez.1402410213 ◽

1987 ◽

Vol 241 (2) ◽

pp. 257-262 ◽

Cited By ~ 50

Author(s):

Jerry L. Hedrick ◽

Nathan J. Wardrip ◽

Trish Berger

Keyword(s):

Zona Pellucida ◽

Macromolecular Composition

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Macromolecular Composition Dictates Receptor and G Protein Selectivity of Regulator of G Protein Signaling (RGS) 7 and 9-2 Protein Complexes in Living Cells

Journal of Biological Chemistry ◽

10.1074/jbc.m113.462283 ◽

2013 ◽

Vol 288 (35) ◽

pp. 25129-25142 ◽

Cited By ~ 30

Author(s):

Ikuo Masuho ◽

Keqiang Xie ◽

Kirill A. Martemyanov

Keyword(s):

G Protein ◽

Protein Complexes ◽

Living Cells ◽

G Protein Signaling ◽

Protein Signaling ◽

Macromolecular Composition

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Macromolecular Composition of Secretions Produced by Human Bronchial Explants

Advances in Experimental Medicine and Biology - Mucus in Health and Disease—II ◽

10.1007/978-1-4615-9254-9_56 ◽

1982 ◽

pp. 357-360 ◽

Cited By ~ 5

Author(s):

Stephen J. Coles ◽

K. R. Bhaskar ◽

Donna Defeudis O’Sullivan ◽

Lynne M. Reid

Keyword(s):

Macromolecular Composition

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Fluorescence Correlation and Cross-Correlation Spectroscopy Unveil Cytoplasmic mRNP Composition and Dynamics

10.1101/2020.07.20.212308 ◽

2020 ◽

Author(s):

Àngels Mateu-Regué ◽

Jan Christiansen ◽

Christian Hellriegel ◽

Finn Cilius Nielsen

Keyword(s):

Life Cycle ◽

Dynamic Analysis ◽

Cross Correlation ◽

Rna Binding ◽

Rna Binding Proteins ◽

Correlation Spectroscopy ◽

Live Cells ◽

Macromolecular Complexes ◽

Fluorescence Correlation ◽

Macromolecular Composition

ABSTRACTUnderstanding the mRNA life cycle requires analysis of the dynamic macromolecular composition and stoichiometry of mRNPs. Fluorescence correlation and cross-correlation spectroscopy (FCS and FCCS) are appealing technologies to study mRNP complexes because they readily provide information about the molecular composition, stoichiometry, heterogeneity and dynamics of the particles. We developed FCS protocols for analysis of live cells and cellular lysates, and demonstrate the feasibility of analysing common cytoplasmic mRNPs composed of core factor YBX1, IMPs (or IGF2BPs) and their interactions with other RNA binding proteins such as PABPC1, ELAVL2 (HuB), STAU1 and FMRP. FCCS corroborated previously reported RNA dependent interactions between the factors and provided an estimate of the relative overlap between the factors in the mRNPs. In this way FCS and FCCS provide a new and useful approach for the quantitative and dynamic analysis of mRNP macromolecular complexes that may complement current biochemical approaches.

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