Low Molecular Weight PEI-Based Vectors via Acid-Labile Ortho Ester Linkage for Improved Gene Delivery

2016 ◽  
Vol 16 (8) ◽  
pp. 1175-1187 ◽  
Author(s):  
Lei Zhang ◽  
Min Yu ◽  
Jun Wang ◽  
Rupei Tang ◽  
Guoqing Yan ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Ortho Ester ◽  
Ester Linkage ◽  
Acid Labile

Acid-labile poly(amino alcohol ortho ester) based on low molecular weight polyethyleneimine for gene delivery

2017 ◽  
Vol 32 (3) ◽  
pp. 349-361 ◽  
Author(s):  
Jun Wang ◽  
Lei Zhang ◽  
Xin Wang ◽  
Shengxiang Fu ◽  
Guoqing Yan
Keyword(s):  
Molecular Weight ◽  
Amino Alcohol ◽  
Water Solubility ◽  
Transfection Efficiency ◽  
Enzymatic Digestion ◽  
Low Molecular Weight ◽  
Hydroxyl Groups ◽  
Ortho Ester ◽  
Human Neuroblastoma ◽  
Acid Labile

A series of acid-labile poly(amino alcohol ortho ester) (POEeis) were synthesized through ring-opening polymerization between diglycidyl ethers with ortho ester bonded and low molecular weight polyethyleneimine by various feed molar ratios. The obtain POEei 1 and POEei 2 exhibited clear kinetic of degradation and condensed plasmid DNA into nanoparticles of suitable sizes (250–300 nm) and positive zeta potentials (+20–30 mV) while protecting DNA from enzymatic digestion. Further, these polymers have uniform distribution of abundant hydroxyl groups, which could improve their water solubility, biocompatibility, and lower protein adsorption. Significantly, ortho ester groups in POEeis main-chains could hydrolyze rapidly at acidic endosomal pH, resulting in intracellular DNA release and diminished material toxicity. MTT assay revealed that all the polymers exhibited much lower cytotoxicity than 25 kDa PEI in the human neuroblastoma SH-SY5Y cells. Moreover, the transfection efficiency of POEei 1 was higher than 25 kDa PEI in serum-free medium or 10% serum medium.

Gene delivery with low molecular weight linear polyethylenimines

2005 ◽  
Vol 7 (10) ◽  
pp. 1287-1298 ◽  
Author(s):  
Miriam Breunig ◽  
Uta Lungwitz ◽  
Renate Liebl ◽  
Claudia Fontanari ◽  
Juergen Klar ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight

Co-liposomes of redox-active alkyl-ferrocene modified low MW branched PEI and DOPE for efficacious gene delivery in serum

2015 ◽  
Vol 3 (11) ◽  
pp. 2318-2330 ◽  
Author(s):  
Krishan Kumar ◽  
Gururaja Vulugundam ◽  
Paturu Kondaiah ◽  
Santanu Bhattacharya
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Redox Active ◽  
Branched Polyethylenimine

Ferrocenylated lipopolymers based on low molecular weight, branched polyethylenimine (BPEI 800 Da) for redox controlled gene delivery.

Polyelectrolyte Complexes of Low Molecular Weight PEI and Citric Acid as Efficient and Nontoxic Vectors for in Vitro and in Vivo Gene Delivery

2016 ◽  
Vol 27 (3) ◽  
pp. 549-561 ◽  
Author(s):  
M. Dolores Giron-Gonzalez ◽  
Rafael Salto-Gonzalez ◽  
F. Javier Lopez-Jaramillo ◽  
Alfonso Salinas-Castillo ◽  
Ana Belen Jodar-Reyes ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Citric Acid ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Polyelectrolyte Complexes ◽  
In Vivo Gene Delivery

Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

2019 ◽  
Vol 7 (4) ◽  
pp. 1716-1728 ◽  
Author(s):  
Huapan Fang ◽  
Lin Lin ◽  
Jie Chen ◽  
Jiayan Wu ◽  
Huayu Tian ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Zinc Ion ◽  
Low Molecular Weight ◽  
Gene Delivery System ◽  
System P

A zinc ion coordination-contained polycationic gene delivery system.

scholarly journals Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents?

Polymers ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 693-718 ◽  
Author(s):  
Olga Samsonova ◽  
Christian Pfeiffer ◽  
Markus Hellmund ◽  
Olivia M. Merkel ◽  
Thomas Kissel
Keyword(s):  
Molecular Weight ◽  
Block Copolymers ◽  
Gene Delivery ◽  
Raft Polymerization ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Viral Gene Delivery

A novel nonviral gene delivery vector: Low-molecular-weight polyethylenimine-graft-ovalbumin

2009 ◽  
Vol 114 (6) ◽  
pp. 3744-3750 ◽  
Author(s):  
Dan Chen ◽  
Yang Liu ◽  
Anna Lehtinen ◽  
Qiying Jiang ◽  
Jun Liu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Low Molecular Weight ◽  
Gene Delivery Vector ◽  
Delivery Vector

Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics

2012 ◽  
Vol 23 (2) ◽  
pp. 222-231 ◽  
Author(s):  
Jisheng Xiao ◽  
Xiaopin Duan ◽  
Qi Yin ◽  
Lingli Chen ◽  
Zhiwen Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight
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