Isolation and characterization of kirsten murine sarcoma virus-transformed mouse keratinocytes resistant to transforming growth factor beta

1990 ◽  
Vol 3 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Nancy J. Sipes ◽  
Russette M. Lyons ◽  
Harold L. Moses
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Isolation And Characterization ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Growth Factor Beta ◽  
Mouse Keratinocytes ◽  
Murine Sarcoma

scholarly journals Characterization of the binding of transforming growth factor-beta 1, -beta 2, and -beta 3 to recombinant beta 1-latency-associated peptide.

1992 ◽  
Vol 6 (5) ◽  
pp. 694-702
Author(s):  
D M Miller ◽  
Y Ogawa ◽  
K K Iwata ◽  
P ten Dijke ◽  
A F Purchio ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Growth Factor Beta ◽  
Beta 2

scholarly journals Characterization of distinct functional domains of transforming growth factor beta.

1993 ◽  
Vol 90 (18) ◽  
pp. 8628-8632 ◽  
Author(s):  
J. K. Burmester ◽  
S. W. Qian ◽  
A. B. Roberts ◽  
A. Huang ◽  
S. Amatayakul-Chantler ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Functional Domains ◽  
Growth Factor Beta

Complex regulation of transforming growth factor beta 1, beta 2, and beta 3 mRNA expression in mouse fibroblasts and keratinocytes by transforming growth factors beta 1 and beta 2

1989 ◽  
Vol 9 (12) ◽  
pp. 5508-5515
Author(s):  
C C Bascom ◽  
J R Wolfshohl ◽  
R J Coffey ◽  
L Madisen ◽  
N R Webb ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Mrna Levels ◽  
Tgf Beta ◽  
Growth Factor Beta ◽  
Beta 2 ◽  
Maximal Induction ◽  
Mouse Keratinocytes

Regulation of transforming growth factor beta 1 (TGF beta 1), TGF beta 2, and TGF beta 3 mRNAs in murine fibroblasts and keratinocytes by TGF beta 1 and TGF beta 2 was studied. In quiescent AKR-2B fibroblasts, in which TGF beta induces delayed stimulation of DNA synthesis, TGF beta 1 autoregulation of TGF beta 1 expression was observed as early as 1 h, with maximal induction (25-fold) after 6 to 12 h. Increased expression of TGF beta 1 mRNA was accompanied by increased TGF beta protein production into conditioned medium of AKR-2B cells. Neither TGF beta 2 nor TGF beta 3 mRNA, however, was significantly induced, but both were apparently down regulated at later times by TGF beta 1. Protein synthesis was not required for autoinduction of TGF beta 1 mRNA in AKR-2B cells. Nuclear run-on analyses and dactinomycin experiments indicated that autoregulation of TGF beta 1 expression is complex, involving both increased transcription and message stabilization. In contrast to TGF beta 1, TGF beta 2 treatment of quiescent AKR-2B cells increased expression of TGF beta 1, TGF beta 2, and TGF beta 3 mRNAs, but with different kinetics. Autoinduction of TGF beta 2 mRNA occurred rapidly with maximal induction at 1 to 3 h, enhanced TGF beta 3 mRNA levels were observed after 3 h, and increased expression of TGF beta 1 occurred later, with maximal mRNA levels obtained after 12 to 24 h. Nuclear run-on analyses indicated that TGF beta 2 regulation of TGF beta 2 and TGF beta 3 mRNA levels is transcriptional, while TGF beta 2 induction of TGF beta 1 expression most likely involves both transcriptional and posttranscriptional controls. In BALB/MK mouse keratinocytes, minimal autoinduction of TGF beta 1 occurred at only the 12- and 24-h time points and protein synthesis was required for this autoinduction. The results of this study provide an example in which TGF beta 1 and TGF beta 2 elicit different responses and demonstrate that expression of TGF beta 1, and TGF beta 3 are regulated differently. The physiological relevance of TGF beta 1 autoinduction in the context of wound healing is discussed.

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