Mutation of cyp19a1b results in sterile males due to efferent duct obstruction in Nile tilapia

2019 ◽  
Vol 86 (9) ◽  
pp. 1224-1235 ◽  
Author(s):  
Xianbo Zhang ◽  
Qianwen Min ◽  
Mengru Li ◽  
Xingyong Liu ◽  
Minghui Li ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Duct Obstruction ◽  
Efferent Duct

scholarly journals Haploinsufficiency of SF-1 Causes Female to Male Sex Reversal in Nile Tilapia, Oreochromis niloticus

Endocrinology ◽  
2016 ◽  
Vol 157 (6) ◽  
pp. 2500-2514 ◽  
Author(s):  
Qing-Ping Xie ◽  
Xue He ◽  
Yi-Ning Sui ◽  
Li-Li Chen ◽  
Li-Na Sun ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Gonadal Dysgenesis ◽  
Nile Tilapia ◽  
Sex Reversal ◽  
Efferent Duct ◽  
Forkhead Box ◽  
Male Sex ◽  
Steroidogenic Cells ◽  
Cytochrome P450 Family ◽  
Estradiol 17Β

Steroidogenic factor-1 (Sf-1) (officially designated nuclear receptor subfamily 5 group A member 1 [NR5A1]) is a master regulator of steroidogenesis and reproduction in mammals. However, its function remains unclear in nonmammalian vertebrates. In the present study, we used immunohistochemistry to detect expression of Sf-1 in the steroidogenic cells, the interstitial, granulosa, and theca cells of the ovary, and the Leydig cells of the testis, in Nile tilapia. Clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (Cas9) cleavage of sf-1 resulted in a high mutation rate in the F0 generation and a phenotype of gonadal dysgenesis and reduced steroidogenic cells in XX and XY fish. Sf-1 deficiency also resulted in decreased cytochrome P450, family 19, subfamily A, polypeptide 1a, forkhead box L2 expression, and serum estradiol-17β in XX fish. In XY fish, Sf-1 deficiency increased cytochrome P450, family 19, subfamily A, polypeptide 1a and forkhead box L2 expression but decreased cytochrome P450, family 11, subfamily B, polypeptide 2 expression and serum 11-ketotestosterone levels. 17α-methyltestosterone treatment successfully rescued the gonadal phenotype of Sf-1-deficient XY fish, as demonstrated by normal spermatogenesis and production of F1 mutants. In contrast, estradiol-17β treatment only partially rescued the gonadal phenotype of Sf-1-deficient XX fish, as demonstrated by the appearance of phase II oocytes. Furthermore, both sf-1+/− F1 XX and XY mutants developed as fertile males, although spermatogenesis was delayed and efferent duct formation was disordered. Our data suggest that Sf-1 is a major regulator of steroidogenesis and reproduction in fish, as it is in mammals. Sf-1 deficiency resulted in gonadal dysgenesis and feminization of XY gonads. However, unlike in mammals, Sf-1 deficiency also resulted in female to male sex reversal in 8.1% of F0 and 92.1% of sf-1+/− F1 in XX fish.

Efferent Duct

2020 ◽  
Author(s):  
Keyword(s):  
Efferent Duct

Congenital nasolacrimal duct obstruction: irrigation or probing?

2000 ◽  
Vol 14 (2) ◽  
pp. 90 ◽  
Author(s):  
Y S Kim ◽  
S C Moon ◽  
K W Yoo
Keyword(s):  
Nasolacrimal Duct ◽  
Nasolacrimal Duct Obstruction ◽  
Congenital Nasolacrimal Duct Obstruction ◽  
Duct Obstruction

Morphological re-description and phylogenetic relationship of five myxosporean species of the family Myxobolidae infecting Nile tilapia

2017 ◽  
Vol 124 (3) ◽  
pp. 201-214 ◽  
Author(s):  
R Abdel-Gaber ◽  
F Abdel-Ghaffar ◽  
S Maher ◽  
AM El-Mallah ◽  
S Al Quraishy ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Nile Tilapia ◽  
The Family ◽  
Relationship Of ◽  
Myxosporean Species

Characterization of Francisella noatunensis subsp. orientalis isolated from Nile tilapia Oreochromis niloticus farmed in Lake Yojoa, Honduras

2019 ◽  
Vol 133 (2) ◽  
pp. 141-145 ◽  
Author(s):  
E Soto ◽  
K Shahin ◽  
JJ Talhami ◽  
MJ Griffin ◽  
A Adams ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

2001 ◽  
Vol 16 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Gracinda De Lourdes Jorge ◽  
Luiz Sergio Leonardi ◽  
Ilka de Fatima Santana Ferreira Boin ◽  
Orlando de Castro e Silva Jr ◽  
Cecilia Amelia Fazzio Escanhoela
Keyword(s):  
Bile Duct ◽  
Morphological Study ◽  
Wistar Rats ◽  
Hepatic Duct ◽  
Control Group ◽  
Sham Operation ◽  
Biliary Cirrhosis ◽  
Technical Failure ◽  
Duct Obstruction ◽  
Duct Ligation

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

Contemporary methods of surgical treatment of nasolacrimal duct obstruction in adults

2017 ◽  
Vol 4 (2) ◽  
pp. 110-115
Author(s):  
Marcin Broda ◽  
Dominika Białas ◽  
Radosław Różycki
Keyword(s):  
Surgical Treatment ◽  
Nasolacrimal Duct ◽  
Nasolacrimal Duct Obstruction ◽  
Duct Obstruction
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