Genetic relatedness of toxic and nontoxic isolates of the marine pennate diatomPseudonitzschia (bacillariophyceae): Phylogenetic analysis of 18S rRNA sequences

1994 ◽  
Vol 2 (4) ◽  
pp. 166-174 ◽  
Author(s):  
Donald J. Douglas ◽  
Denise Landry ◽  
Susan E. Douglas
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Genetic Relatedness ◽  
Rrna Sequences

scholarly journals Hepatozoon caimani in Caiman crocodilus yacare (Crocodylia, Alligatoridae) from North Pantanal, Brazil

2017 ◽  
Vol 26 (3) ◽  
pp. 352-358 ◽  
Author(s):  
Andréa Bouer ◽  
Marcos Rogério André ◽  
Luiz Ricardo Gonçalves ◽  
Mayara de Cássia Luzzi ◽  
Juliana Paula de Oliveira ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Molecular Techniques ◽  
Mato Grosso ◽  
Caiman Crocodilus ◽  
Blood Smears ◽  
High Prevalence ◽  
Hepatozoon Species ◽  
Free Ranging ◽  
Rrna Sequences

Abstract Hepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal.

Thraustochytrids are chromists, not Fungi: 18s rRNA signatures of Heterokonta

1994 ◽  
Vol 346 (1318) ◽  
pp. 387-397 ◽  
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Rrna Genes ◽  
Evolutionary Relationships ◽  
Signature Sequences ◽  
A Cell ◽  
Chromophyte Algae ◽  
Marine Protists ◽  
18S Rrna Genes ◽  
Rrna Sequences

Thraustochytrids are a neglected group of non-photosynthetic marine protists characterized by the presence of a sagenogenetosome, an ectoplasmic net and a cell wall composed of non-cellulosic scales. Though originally classified as fungi, they are ultrastructurally closest to the labyrinthulids, which have often been treated as protozoa, but are now usually considered to be more closely related to the heterokont algae. In order to clarify their controversial taxonomic position and evolutionary relationships, we have cloned and sequenced the 18s rRNA genes of the thraustochytrids Thraustochytrium kinnei and Ulkenia profunda . Phylogenetic analysis by several methods confirms earlier suggestions based on ultrastructure that thraustochytrids constitute a deeply divergent branch of the phylum Heterokonta, which is currently classified in the kingdom Chromista. The phylum Heterokonta also includes numerous chromophyte algae (e.g. brown algae and diatoms) as well as the oomycetes. Heterokont 18s rRNA sequences can be distinguished from those of all other eukaryotes by having an AU not a UA base pair at the very base of helix 47 in the V9 region. We also tabulate signature sequences that can be used to characterize 18s rRNA sequences from each of the major heterokont taxa.

scholarly journals Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis globosa (Prymnesiophyceae)

2004 ◽  
Vol 70 (6) ◽  
pp. 3700-3705 ◽  
Author(s):  
C. P. D. Brussaard ◽  
S. M. Short ◽  
C. M. Frederickson ◽  
C. A. Suttle
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Coastal Waters ◽  
Common Ancestor ◽  
Genetic Relatedness ◽  
Amino Acid Sequences ◽  
Polymerase Gene ◽  
Phaeocystis Globosa ◽  
The Family ◽  
Algal Host

ABSTRACT Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved.

scholarly journals Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

2021 ◽  
Author(s):  
Saowapha Surawut ◽  
Sorasak Nak-aim ◽  
Chutapa Kunsook ◽  
Laddawan Kamhaengkul ◽  
Pornpimon Kanjanavas ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Genetic Relatedness ◽  
Sequence Similarity ◽  
Its Region ◽  
Bioactive Compound ◽  
Rubber Plantation ◽  
Mushroom Species ◽  
Reference Strains

Abstract Ascomycetes mushrooms are fungi that produce ascospores in asci and some with perithecia. Not only they have a role of decomposer in ecology but also produced some bioactive compound, anti-microbial activity, and cytotoxicity. This study aims to explore the diversity of ascomycetes mushroom species in para rubber plantations and to identify them by morphological and sequence analysis of the internal transcribed spacer (ITS) region. The results found ascomycetes mushroom consist of Trichoderma pezizoides (RP1, % identity 98.79, DQ835513.1), Daldinia eschscholtzii (RP2, % identity 100, MN310384.1), Cookeina sulcipes (RP3, % identity 98.44, KY094620.1), Cookeina garethjonesii (RP4, % identity 99.06, KY094622.1), Cookeina tricholoma (RP5, % identity 100, KY094619.1) and Xylaria terricola (RP6, % identity 88.42, MF577038.1). Most of the ascomycetes in this study have previously been described in Thailand except Xylaria terricola. Additionally, phylogenetic analysis of ascomycetes mushroom showed high genetic relatedness with reference strains. Therefore, the sequence similarity and phylogenetic analysis confirmed the identity of six ascomycetes mushroom species, and further study of bioactive compound from these mushrooms may be investigated for other applications.

scholarly journals Genetic Diversity and Zoonotic Potential of Blastocystis in Korean Water Deer, Hydropotes inermis argyropus

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 955
Author(s):  
Kyoo-Tae Kim ◽  
Gyeonguk Noh ◽  
Haeseung Lee ◽  
Seon-Hee Kim ◽  
Hyesung Jeong ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Intestinal Tract ◽  
Protozoan Parasite ◽  
Domestic Animals ◽  
Nucleotide Sequencing ◽  
Zoonotic Potential ◽  
Infection Status ◽  
Korean Water Deer

Blastocystis is a protozoan parasite commonly detected in the intestinal tract of humans and animals. It has been actively studied worldwide; however, information on Blastocystis is limited in Korea. Because there is an increasing concern about the contact between wildlife and domestic animals or humans, we assessed the infection status and zoonotic potential of Blastocystis in Korean water deer (KWD, Hydropotes inermis argyropus) using genotyping and phylogenetic analysis. A total of 125 fresh fecal samples were collected from KWD which were killed by vehicles on highways or roadsides in this study. Among the 125 samples, 51 (40.8%) were PCR positive. We performed nucleotide sequencing and phylogenetic analysis of 26 of the 51 PCR-positive samples. By analyzing Blastocystis 18S rRNA, two subtypes (ST4 and ST14) were identified in this study. Of the 26 samples analyzed, 25 were identified as ST14 and one as ST4. Infection of ST14 in humans has not been reported. Although only one ST4 sample was detected in this study, ST4 has zoonotic potential without showing ruminant specificity. Thus, continuous attention should be provided to the potential of transmission between wildlife and domestic animals and humans.

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