The Bayesian phylogenetic analysis of the 18S rRNA sequences from the main lineages of Trichophora (Insecta: Heteroptera: Pentatomomorpha)

Abstract Hepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal.

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Thraustochytrids are chromists, not Fungi: 18s rRNA signatures of Heterokonta

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.1994.0156 ◽

1994 ◽

Vol 346 (1318) ◽

pp. 387-397 ◽

Cited By ~ 90

Keyword(s):

Phylogenetic Analysis ◽

18S Rrna ◽

Rrna Genes ◽

Evolutionary Relationships ◽

Signature Sequences ◽

A Cell ◽

Chromophyte Algae ◽

Marine Protists ◽

18S Rrna Genes ◽

Rrna Sequences

Thraustochytrids are a neglected group of non-photosynthetic marine protists characterized by the presence of a sagenogenetosome, an ectoplasmic net and a cell wall composed of non-cellulosic scales. Though originally classified as fungi, they are ultrastructurally closest to the labyrinthulids, which have often been treated as protozoa, but are now usually considered to be more closely related to the heterokont algae. In order to clarify their controversial taxonomic position and evolutionary relationships, we have cloned and sequenced the 18s rRNA genes of the thraustochytrids Thraustochytrium kinnei and Ulkenia profunda . Phylogenetic analysis by several methods confirms earlier suggestions based on ultrastructure that thraustochytrids constitute a deeply divergent branch of the phylum Heterokonta, which is currently classified in the kingdom Chromista. The phylum Heterokonta also includes numerous chromophyte algae (e.g. brown algae and diatoms) as well as the oomycetes. Heterokont 18s rRNA sequences can be distinguished from those of all other eukaryotes by having an AU not a UA base pair at the very base of helix 47 in the V9 region. We also tabulate signature sequences that can be used to characterize 18s rRNA sequences from each of the major heterokont taxa.

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Lassa Virus Circulation in Small Mammal Populations in Bo District, Sierra Leone

Biology ◽

10.3390/biology10010028 ◽

2021 ◽

Vol 10 (1) ◽

pp. 28

Author(s):

Umaru Bangura ◽

Jacob Buanie ◽

Joyce Lamin ◽

Christopher Davis ◽

Gédéon Ngiala Bongo ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Sierra Leone ◽

Small Mammal ◽

Hemorrhagic Fever ◽

Lassa Fever ◽

Lassa Virus ◽

Sierra Leonean ◽

Bayesian Phylogenetic Analysis ◽

Prevalent Species ◽

Lineage Iv

Lassa fever is a viral hemorrhagic fever caused by the Lassa virus LASV, which was first isolated in the rodent Mastomys natalensis in 1974 in Kenema, Sierra Leone. As little is known about the abundance and the presence of LASV in rodents living in the Bo area, we carried out a small mammal longitudinal population survey. A standardized trapping session was performed in various habitats and seasons in six villages over two years (2014–2016) and samples collected were tested for arenavirus IgG and LASV. A Bayesian phylogenetic analysis was performed on sequences identified by PCR. A total of 1490 small mammals were collected, and 16 rodent species were identified, with M. natalensis (355, 24%) found to be the most prevalent species. Forty-one (2.8%) samples were IgG positive, and 31 of these were trapped in homes and 10 in surrounding vegetation. Twenty-nine of 41 seropositive rodents were M. natalensis. We detected four LASV by PCR in two villages, all found in M. natalensis. Phylogenetic analysis showed that the sequences were distributed within the Sierra Leonean clade within lineage IV, distinguishing a Bo sub-clade older than a Kenema sub-clade. Compared to other settings, we found a low abundance of M. natalensis and a low circulation of LASV in rodents in villages around Bo district.

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Case of Human Infestation with Dermanyssus gallinae (Poultry Red Mite) from Swallows (Hirundinidae)

Pathogens ◽

10.3390/pathogens10030299 ◽

2021 ◽

Vol 10 (3) ◽

pp. 299

Author(s):

Georgios Sioutas ◽

Styliani Minoudi ◽

Katerina Tiligada ◽

Caterina Chliva ◽

Alexandros Triantafyllidis ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Cytochrome C ◽

Cytochrome C Oxidase ◽

Clinical Symptoms ◽

Skin Lesions ◽

Coi Gene ◽

Dermanyssus Gallinae ◽

Poultry Red Mite ◽

Human Form ◽

Bayesian Phylogenetic Analysis

Dermanyssus gallinae (the poultry red mite, PRM) is an important ectoparasite in the laying hen industry. PRM can also infest humans, causing gamasoidosis, which is manifested as skin lesions characterized by rash and itching. Recently, there has been an increase in the reported number of human infestation cases with D. gallinae, mostly associated with the proliferation of pigeons in cities where they build their nests. The human form of the disease has not been linked to swallows (Hirundinidae) before. In this report, we describe an incident of human gamasoidosis linked to a nest of swallows built on the window ledge of an apartment in the island of Kefalonia, Greece. Mites were identified as D. gallinae using morphological keys and amplifying the Cytochrome C oxidase subunit I (COI) gene by PCR. Bayesian phylogenetic analysis and median-joining network supported the identification of three PRM haplogroups and the haplotype isolated from swallows was identical to three PRM sequences isolated from hens in Portugal. The patient was treated with topical corticosteroids, while the house was sprayed with deltamethrin. After one week, the mites disappeared and clinical symptoms subsided. The current study is the first report of human gamasoidosis from PRM found in swallows’ nest.

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A biologist’s guide to Bayesian phylogenetic analysis

Nature Ecology & Evolution ◽

10.1038/s41559-017-0280-x ◽

2017 ◽

Vol 1 (10) ◽

pp. 1446-1454 ◽

Cited By ~ 51

Author(s):

Fabrícia F. Nascimento ◽

Mario dos Reis ◽

Ziheng Yang

Keyword(s):

Phylogenetic Analysis ◽

Bayesian Phylogenetic Analysis

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Reassignment of 'Triplophysa' jiarongensis (Cypriniformes: Nemacheilidae) to Oreonectes Günther, 1868

Zootaxa ◽

10.11646/zootaxa.4565.3.6 ◽

2019 ◽

Vol 4565 (3) ◽

pp. 398 ◽

Cited By ~ 1

Author(s):

XUAN LIU ◽

HUAIQING DENG ◽

ZHIJIN LIU ◽

JIANG ZHOU

Keyword(s):

Phylogenetic Analysis ◽

Short Distance ◽

River System ◽

Genetic Distances ◽

Molecular Evidence ◽

Karst Cave ◽

Bayesian Phylogenetic Analysis ◽

As Species ◽

Secondary Sexual Dimorphism ◽

Underground River

Specimens identified as Oreonectes jiarongensis (Cypriniformes: Nemacheilidae) were collected from a karst cave in Jiarong Town and Banzhai Township, Libo County, Guizhou, China. Several lines of morphological and molecular evidence suggested that this species was similar to species of Oreonectes Günther 1868 and not closely related to species of Triplophysa Rendal, 1933. The anterior and posterior nostrils of 'Triplophysa' jiarongensis were separated by a short distance, and there was no secondary sexual dimorphism in male specimens. The Bayesian phylogenetic analysis based on cytochrome b recovered 'T.' jiarongensis in a well-supported clade with Oreonectes daqikongensis, O. shuilongensis, O. furcocaudalis and O. platycephalus, sister to the Triplophysa clade. In addition, the genetic distances between 'T.' jiarongensis and species of Oreonectes were low (O. daqikongensis: 0.114; O. shuilongensis: 0.106; O. platycephalus: 0.180), while distances to species of Triplophysa were higher (T. dorsalis: 0.233; T. yarkandensis: 0.282). Therefore, we reassign 'T.' jiarongensis to Oreonectes. Based on this result, it is now clear that species of both Oreonectes and Triplophysa inhabit the same underground river system in Guizhou. However, as species of Oreonectes are only known from the southern part of Guizhou, it may be that O. jiarongensis inhabits the most northerly part of the range of this genus.

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