scholarly journals Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

2021 ◽  
Author(s):  
Saowapha Surawut ◽  
Sorasak Nak-aim ◽  
Chutapa Kunsook ◽  
Laddawan Kamhaengkul ◽  
Pornpimon Kanjanavas ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Genetic Relatedness ◽  
Sequence Similarity ◽  
Its Region ◽  
Bioactive Compound ◽  
Rubber Plantation ◽  
Mushroom Species ◽  
Reference Strains

Abstract Ascomycetes mushrooms are fungi that produce ascospores in asci and some with perithecia. Not only they have a role of decomposer in ecology but also produced some bioactive compound, anti-microbial activity, and cytotoxicity. This study aims to explore the diversity of ascomycetes mushroom species in para rubber plantations and to identify them by morphological and sequence analysis of the internal transcribed spacer (ITS) region. The results found ascomycetes mushroom consist of Trichoderma pezizoides (RP1, % identity 98.79, DQ835513.1), Daldinia eschscholtzii (RP2, % identity 100, MN310384.1), Cookeina sulcipes (RP3, % identity 98.44, KY094620.1), Cookeina garethjonesii (RP4, % identity 99.06, KY094622.1), Cookeina tricholoma (RP5, % identity 100, KY094619.1) and Xylaria terricola (RP6, % identity 88.42, MF577038.1). Most of the ascomycetes in this study have previously been described in Thailand except Xylaria terricola. Additionally, phylogenetic analysis of ascomycetes mushroom showed high genetic relatedness with reference strains. Therefore, the sequence similarity and phylogenetic analysis confirmed the identity of six ascomycetes mushroom species, and further study of bioactive compound from these mushrooms may be investigated for other applications.

scholarly journals Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China

MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 103-139
Author(s):  
Guo-Jie Li ◽  
Shou-Mian Li ◽  
Bart Buyck ◽  
Shi-Yi Zhao ◽  
Xue-Jiao Xie ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Its Region ◽  
Line Drawings ◽  
Phylogenetic Data ◽  
Three New Species ◽  
First Time

Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.

Two new rock-inhabiting species of Cyphellophora from karst habitats in China

Phytotaxa ◽  
2019 ◽  
Vol 397 (1) ◽  
pp. 23
Author(s):  
WEI SUN ◽  
BINGJIE LIU ◽  
RONG FU ◽  
XINGZHONG LIU ◽  
MEICHUN XIANG
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Its Region ◽  
Closely Related Species ◽  
Two New Species ◽  
Rock Inhabiting Fungi ◽  
Karst Habitats

During survey on rock-inhabiting fungi from karst habitats in Guizhou, China, two new species in Cyphellophora were discovered and identified. Phylogenetic analysis based on combined sequences of the nuclear large subunit (nucLSU) and internal transcribed spacer (ITS) region of ribosomal DNA revealed that the tested isolates, clustered into two clades that well affiliated in the genus of Cyphellophora. Morphological characteristics were also supported the estabolishment of the new species. Herewith Cyphellophora botryose sp. nov. and Cyphellophora guizhouensis sp. nov. were described and their differences from closely related species were discussed.

scholarly journals Sarcocystis sp. parasites in the Mexican Great-tailed Grackle (Quiscalus mexicanus), Bronzed Cowbird (Molothrus aeneus), and Stripe-headed Sparrow (Aimophila ruficauda)

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Félix Domingo Sánchez-Godoy ◽  
Fernando Chávez-Maya ◽  
Adriana Méndez-Bernal ◽  
Gary García-Espinosa ◽  
Cristina Guerrero-Molina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Sequence Similarity ◽  
Bird Species ◽  
The United States ◽  
Histopathological Analysis ◽  
Biological Studies ◽  
Pcr Rflp ◽  
Molothrus Aeneus ◽  
Specific Fragment

The objective of this study was to describe the morphological and ultraestructural characteristics, the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) results, the sequences and the phylogenetic analysis of a specific fragment of internal transcribed spacer 1 (ITS-1), amplified using the 25/396 primers, of the Sarcocystis sp. parasites identified in the muscles of wild great-tailed grackles, bronzed cowbirds, and stripe-headed sparrows in Mexico. Fifteen birds with sarcocystosis in their skeletal muscles were studied: 7 great-tailed grackles (Quiscalus mexicanus), 6 bronzed cowbirds (Molothrus aeneus), and 2 stripe-headed sparrows (Aimophila ruficauda). Histopathological analysis revealed thin-walled mature parasite cysts. Ultrastructurally, the cyst wall consisted of a granular layer with villar protrusions and numerous microtubules. The bradyzoites measured 4.1 × 1.6 µm, and micronemes appeared in the anterior third of the conoid. For molecular identification, PCR-RFLP was performed using sequences of a specific fragment of internal transcribed spacer 1 (ITS-1) using the primers 25/396 and Hinf I. Hind III did not cut this fragment. The sequencing results indicated a 100% similarity among the Sarcocystis parasites from the three bird species, and a BLAST search revealed 96% sequence similarity with S. neurona. The phylogenetic analysis shows that the sequences studied are topologically distant to those sequences reported for S. neurona in the United States and in South America and are not related to any group previously reported. Although our morphological and molecular analysis data provide strong evidence that S. neurona uses these bird species as intermediate hosts, future molecular studies with additional DNA fragments, combined with biological studies, will ultimately allow us to convincingly identify these parasites. This is the first report of a Sarcocystis sp. parasite in wild birds in Mexico that may be S. neurona.

Infundibulicybe macrospora sp. nov., a new and noteworthy species from Himalayan moist temperate forests of Pakistan

Phytotaxa ◽  
2020 ◽  
Vol 452 (4) ◽  
pp. 268-277
Author(s):  
MUHAMMAD ALI ◽  
JUNAID KHAN ◽  
HIRA BASHIR ◽  
ABDUL REHMAN NIAZI ◽  
HASSAN SHER ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Temperate Forests ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Reddish Orange ◽  
Light Orange

Infundibulicybe macrospora, a new species, is described and illustrated from the Himalayan moist temperate forests of Pakistan. The new species can be differentiated from close allies by its reddish orange to light orange pileus, whitish lamellae and stipe, and larger and broader basidiospores. The new species is also supported by molecular phylogenetic analysis inferred from internal transcribed spacer (ITS) region.

scholarly journals Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

2000 ◽  
Vol 38 (4) ◽  
pp. 1510-1515 ◽  
Author(s):  
Travis Henry ◽  
Peter C. Iwen ◽  
Steven H. Hinrichs
Keyword(s):  
Internal Transcribed Spacer ◽  
Its Region ◽  
Diagnostic Value ◽  
Species Level ◽  
Clinical Isolates ◽  
Rrna Genes ◽  
28S Rrna ◽  
Accurate Identification ◽  
Invasive Mold Infections ◽  
Reference Strains

Aspergillus species are the most frequent cause of invasive mold infections in immunocompromised patients. Although over 180 species are found within the genus, 3 species, Aspergillus flavus, A. fumigatus, and A. terreus, account for most cases of invasive aspergillosis (IA), with A. nidulans, A. niger, and A. ustus being rare causes of IA. The ability to distinguish between the various clinically relevant Aspergillus species may have diagnostic value, as certain species are associated with higher mortality and increased virulence and vary in their resistance to antifungal therapy. A method to identify Aspergillus at the species level and differentiate it from other true pathogenic and opportunistic molds was developed using the 18S and 28S rRNA genes for primer binding sites. The contiguous internal transcribed spacer (ITS) region, ITS 1–5.8S–ITS 2, from referenced strains and clinical isolates of aspergilli and other fungi were amplified, sequenced, and compared with non-reference strain sequences in GenBank. ITS amplicons fromAspergillus species ranged in size from 565 to 613 bp. Comparison of reference strains and GenBank sequences demonstrated that both ITS 1 and ITS 2 regions were needed for accurate identification ofAspergillus at the species level. Intraspecies variation among clinical isolates and reference strains was minimal. Sixteen other pathogenic molds demonstrated less than 89% similarity withAspergillus ITS 1 and 2 sequences. A blind study of 11 clinical isolates was performed, and each was correctly identified. Clinical application of this approach may allow for earlier diagnosis and selection of effective antifungal agents for patients with IA.

scholarly journals Cortinarius gueneri: A new species from the Euro-Siberian Floristic Region of Turkey

Karstenia ◽  
2021 ◽  
pp. 46-54
Author(s):  
Ertugrul Sesli
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Molecular Data ◽  
Full Description ◽  
Short Discussion ◽  
Morphological And Molecular Data ◽  
A New Species

<em>Cortinarius gueneri</em> (subgen. <em>Telamonia</em> sect. <em>Laeti</em>) is described as a new species from Turkey based on morphological and molecular data. It can be recognized by the somewhat irregular conical to campanulate, translucent-striate, rusty brown pileus; pale yellowish to cinnamon brown broadly attached lamellae; pale salmon to pinkish brown stipe; narrowly amygdaloid to ellipsoid basidiospores; and its putative association with <em>Carpinus orientalis</em>. Full description of the new species is given with field photos, microscopic illustrations, and a short discussion. Phylogenetic analysis of the internal transcribed spacer (ITS) region is also provided.

Three new species of Calocybella from India based on morphology and molecular phylogeny

Phytotaxa ◽  
2016 ◽  
Vol 255 (2) ◽  
pp. 133 ◽  
Author(s):  
K. P. DEEPNA LATHA ◽  
K. N. ANIL RAJ ◽  
THUSHARA CHEROLIL ◽  
SHAHINA A. SHARAFUDHEEN ◽  
PATINJAREVEETTIL MANIMOHAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogeny ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Its Region ◽  
First Record ◽  
Three New Species ◽  
Kerala State ◽  
Blastn Search

Three new species of Calocybella, C. lohitha, C. babruka and C. swetha are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions of these species are provided along with photo-illustrations and comparisons with phenetically similar and phylogenetically related species. Nuclear ribosomal internal transcribed spacer (ITS) region of the three species were sequenced and analyzed. In addition, nuclear ribosomal large subunit (nrLSU) region of Calocybella swetha was sequenced and used in BLASTn search to find similar sequences. ITS-based phylogenetic analysis using Maximum likelihood (ML) method supported both the novelty of these species and their placement within the genus. The discovery of these three species forms the first record of Calocybella outside Europe.

Suillus marginielevatus, a new species and S. triacicularis, a new record from Western Himalaya, Pakistan

Phytotaxa ◽  
2015 ◽  
Vol 203 (2) ◽  
pp. 169 ◽  
Author(s):  
Samina Sarwar ◽  
MALKA SABA ◽  
ABDUL N. KHALID ◽  
BRYN M. DENTINGER
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Phylogenetic Tree ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Temperate Forests ◽  
Its Region ◽  
Western Himalaya ◽  
Line Drawings ◽  
A New Species

Suillus marginielevatus sp. nov. and S. triacicularis are reported from Himalayan moist temperate forests of Pakistan in association with conifers. Morphologically S. marginielevatus is close to S. sibiricus, S. granulatus and S. intermedius but it can be distinguished by uplifted pileus margin and curved stipe with no ring at all stages. Phylogenetic analysis of internal transcribed spacer (ITS) region showed that this species form separate clade from other closely related Suillus species. Suillus triacicularis represents a new record for Pakistani mycobiota. Field photographs of fresh basidocarps and line drawings of microcharacters are provided along with phylogenetic tree.

Heliocybe villosa sp. nov., a new member to the genus Heliocybe (Gloeophyllales)

Phytotaxa ◽  
2018 ◽  
Vol 349 (2) ◽  
pp. 173 ◽  
Author(s):  
MING ZHANG ◽  
TAI-HUI LI ◽  
BIN SONG
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Its Region ◽  
Morphological Characters ◽  
Molecular Data ◽  
Hyphal System

Heliocybe villosa sp. nov. from southern China, is described, illustrated and discussed here based on its distinct morphological characters and molecular data. It is characterized by a yellowish brown pileus densely covered with orange brown villous hairs or flocci, white and close lamellae with serrated edge, broadly cylindric to subreniform basidiospores 7–9 × 3–3.5 μm, and monomitic hyphal system without clamp connections. Phylogenetic analysis based on the internal transcribed spacer (ITS) region further confirmed its placement in Heliocybe, and the second recorded species for this genus.

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