The relationship of the oxidation-reduction potentials developed in bacterial cultures to the production of hydrogen peroxide

To examine the relationship of the formation of biofilm (adherent bacterial microcolonies) to recurrent peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPO) we examined the surfaces of 25 recovered Tenckhoff (T) catheters by scanning electron microscopy (SEM) and microbiological examination of scrapings. Twelve catheters had been removed after successful transplantation; the patients had not been dialyzed for three months and had no evidence of peritonitis during that period (control group), and only seven had experienced peritonitis (I or 2 episodes) before transplantation. All 13 patients in the study group had experienced repeated episodes of peritonitis (range 3–13, average, 5.6). We detected no significant differences between the control and study groups with respect to the percentage of catheter-surface covered by biofilm, or the proportion of catheter segments whose scrapings yielded positive bacterial cultures. It is concluded that large areas of the T catheters of CAPO patients are colonized by bacterial biofilms but only active dialysis predisposes to recurrent peritonitis.

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The Relationship of Hydrogen Peroxide-Producing Lactobacilli to Bacterial Vaginosis and Genital Microflora in Pregnant Women

Obstetrics and Gynecology ◽

10.1097/00006250-199203000-00008 ◽

1992 ◽

Vol 79 (3) ◽

pp. 369-373 ◽

Cited By ~ 122

Author(s):

SHARON L. HILLIER ◽

MARIJANE A. KROHN ◽

SEYMOUR J. KLEBANOFF ◽

DAVID A. ESCHENBACH

Keyword(s):

Hydrogen Peroxide ◽

Bacterial Vaginosis ◽

Pregnant Women ◽

Relationship Of ◽

The Relationship

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The relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women

International Journal of Gynecology & Obstetrics ◽

10.1016/0020-7292(92)90920-e ◽

1992 ◽

Vol 39 (2) ◽

pp. 152-152

Author(s):

SL Hillier ◽

ME Krohn ◽

SJ Klebanoff ◽

DA Eschenbach

Keyword(s):

Hydrogen Peroxide ◽

Bacterial Vaginosis ◽

Pregnant Women ◽

Relationship Of ◽

The Relationship

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Sanitation of a South African Forestry Nursery Contaminated with Fusarium circinatum Using Hydrogen Peroxide at Specific Oxidation Reduction Potentials

Plant Disease ◽

10.1094/pdis-05-11-0432 ◽

2012 ◽

Vol 96 (6) ◽

pp. 875-880 ◽

Cited By ~ 11

Author(s):

Schalk J. P. Van Wyk ◽

Anne-Laure Boutigny ◽

Teresa A. Coutinho ◽

Altus Viljoen

Keyword(s):

Hydrogen Peroxide ◽

Irrigation Water ◽

Economic Losses ◽

Western Cape ◽

Fusarium Circinatum ◽

Reduction Potentials ◽

Oxidation Reduction ◽

Pine Seedlings ◽

Species Specific ◽

Specific Oxidation

Pitch canker, caused by Fusarium circinatum, was first reported in a forestry nursery in the Mpumalanga Province of South Africa in 1990, and it has since spread to almost all forestry nurseries in the country, where it causes significant economic losses. The aim of the current study was to (i) identify sources of F. circinatum contamination in the Karatara forestry nursery in the Western Cape Province and (ii) manage the disease by implementing an oxidation reduction potential (ORP)-based sanitation method using hydrogen peroxide. The irrigation water, planting tray inserts and seeds were screened for fungal contamination. Fusarium circinatum colonies were identified morphologically and confirmed by polymerase chain reaction using species-specific primers. Both the irrigation water and planting tray inserts served as sources of inoculum that introduced the pathogen into the nursery. The irrigation water was amended with hydrogen peroxide at an ORP level of 400 mV for an exposure time of 6 h because it was observed that such a treatment effectively killed all F. circinatum spores and was not phytotoxic to pine seedlings under laboratory conditions. In addition, the contaminated planting tray inserts were cleaned in water amended with hydrogen peroxide at an ORP value of 360 mV for 6 h, which was shown to efficiently eliminate all inoculum from planting tray inserts. Since the introduction of the ORP-based sanitation method at Karatara nursery, losses of pine seedlings were reduced to insignificant levels, and field losses were minimized.

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