Monoclonal antibodies to blood group isoantigens: An alternative marker to factor VIII related antigen for benign and malignant vascular endothelial cells

1985 ◽  
Vol 147 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Timothy J. Stephenson ◽  
Patricia M. Mills
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Factor Viii ◽  
Blood Group ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Factor Viii Related Antigen

scholarly journals Localization of factor-VIII-related antigen in human vascular subendothelium

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 752-756
Author(s):  
JH Rand ◽  
II Sussman ◽  
RE Gordon ◽  
SV Chu ◽  
V Solomon
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Blood Vessel ◽  
Factor Viii ◽  
Platelet Adhesion ◽  
Vascular Endothelial Cells ◽  
Staining Technique ◽  
Immunofluorescent Staining ◽  
Vascular Endothelial ◽  
Factor Viii Related Antigen

Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

scholarly journals Localization of factor-VIII-related antigen in human vascular subendothelium

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 752-756 ◽  
Author(s):  
JH Rand ◽  
II Sussman ◽  
RE Gordon ◽  
SV Chu ◽  
V Solomon
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Blood Vessel ◽  
Factor Viii ◽  
Platelet Adhesion ◽  
Vascular Endothelial Cells ◽  
Staining Technique ◽  
Immunofluorescent Staining ◽  
Vascular Endothelial ◽  
Factor Viii Related Antigen

Abstract Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

The Endothelial Cell and the Factor VIII Bypassing Activity of Prothrombin Complex Concentrate

1988 ◽  
Vol 60 (02) ◽  
pp. 226-229 ◽  
Author(s):  
Jerome M Teitel ◽  
Hong-Yu Ni ◽  
John J Freedman ◽  
M Bernadette Garvey
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Factor Viii ◽  
Prothrombin Complex Concentrate ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Monolayer Cultures ◽  
Coagulant Activity ◽  
Time Courses ◽  
Privileged Site

SummarySome classical hemophiliacs have a paradoxical hemostatic response to prothrombin complex concentrate (PCC). We hypothesized that vascular endothelial cells (EC) may contribute to this “factor VIII bypassing activity”. When PCC were incubated with suspensions or monolayer cultures of EC, they acquired the ability to partially bypass the defect of factor VIII deficient plasma. This factor VIII bypassing activity distributed with EC and not with the supernatant PCC, and was not a general property of intravascular cells. The effect of PCC was even more dramatic on fixed EC monolayers, which became procoagulant after incubation with PCC. The time courses of association and dissociation of the PCC-derived factor VIII bypassing activity of fixed and viable EC monolayers were both rapid. We conclude that EC may provide a privileged site for sequestration of constituents of PCC which express coagulant activity and which bypass the abnormality of factor VIII deficient plasma.

Monoclonal Antibodies to Vascular Endothelial Cells - A Possible Tool for Detecting Endothelial Damage

1985 ◽  
Vol 69 (s12) ◽  
pp. 80P-80P
Author(s):  
D.P. de Bono ◽  
S. Pringle
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Vascular Endothelial Cells ◽  
Endothelial Damage ◽  
Vascular Endothelial

Production of two novel monoclonal antibodies that distinguish mouse lymphatic and blood vascular endothelial cells

2006 ◽  
Vol 211 (5) ◽  
pp. 379-393 ◽  
Author(s):  
Taichi Ezaki ◽  
Kazuhiko Kuwahara ◽  
Shunichi Morikawa ◽  
Kazuhiko Shimizu ◽  
Nobuo Sakaguchi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial

Lectins as markers of endothelial cells: comparative study between human and animal cells

1988 ◽  
Vol 22 (2) ◽  
pp. 135-140 ◽  
Author(s):  
F. Roussel ◽  
J. Dalion
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Comparative Study ◽  
Vascular Endothelial Cells ◽  
Human Cells ◽  
Point Of View ◽  
Laboratory Animals ◽  
Vascular Endothelial ◽  
Animal Cells ◽  
Common Laboratory

Vascular endothelial cells were labelled with 10 vegetal lectins and 3 more monoclonal antibodies antiblood group ABO substances, in major organs of 14 common laboratory animals. After fixation in PLPa and paraffin embedding, cells were examined to determine their likeness to human cells. The most interesting reactive used was EEA, whose positivity defines upper mammalians. Blood B substance positivity and CSA negativity defines primates among which man is unique and defined by UEA I positivity and variability in ABO substance. CSA positivity defines non-primate upper mammalians. Rodents and birds were negative with all reactives tested. From the histochemical point of view, the animals closest to humans are monkeys, followed by swine and oxen, then by cat and dog and lastly by sheep. Rodents appear unrelated to humans in this system.

scholarly journals Factor VIII-Related Antigen Detects Phenotypic Change of Sinusoidal to Vascular Endothelium in Hepatic Fibrosis of Elderly Cadavers

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Ki M. Mak ◽  
Priya Sehgal ◽  
Cynthia K. Harris
Keyword(s):  
Endothelial Cells ◽  
Liver Fibrosis ◽  
Factor Viii ◽  
Hepatic Fibrosis ◽  
Vascular Endothelium ◽  
Vascular Endothelial Cells ◽  
Positive Control ◽  
Phenotypic Change ◽  
Phenotypic Marker ◽  
Factor Viii Related Antigen

In advanced stages of hepatic fibrosis, the liver sinusoidal endothelium transforms to vascular endothelium with accompanying expression of factor VIII-related antigen (FVIIIRAg), a phenotypic marker of vascular endothelial cells. Liver fibrosis has been shown to be associated with aging and was found to be prevalent in elderly cadavers. Using immunohistochemistry, we studied FVIIIRAg expression in the livers of elderly cadavers with progressive stages of fibrosis. The vascular endothelium of portal tracts and central veins was stained for FVIIIRAg, providing an internal positive control. The incidence of FVIIIRAg expression was low in the sinusoids of livers that showed minimal fibrosis or perisinusoidal fibrosis but was increased in livers with advanced fibrosis (i.e., septa formation, bridging fibrosis, and cirrhosis). FVIIIRAg positive sinusoidal endothelial cells were distributed in loose aggregates in the periportal, periseptal, and midlobular parenchyma and were found less frequently in the centrilobular area. FVIIIRAg immune deposits appeared patchy and discontinuous along the sinusoidal lining, likely representing focalized transformation of sinusoidal to vascular endothelium. There was a discrete localization of FVIIIRAg immunoreactivity in the foci of severe parenchymal fibrosis. Conclusion. FVIIIRAg is a reliable marker for detecting the transformation of sinusoidal to vascular endothelium in advanced liver fibrosis in elderly cadavers.

Inhibitory effects of TTP sera on binding of anti-platelet glycoprotein IIb-IIIa monoclonal antibodies to human vascular endothelial cells

1987 ◽  
Vol 25 (1) ◽  
pp. 115-118 ◽  
Author(s):  
Tokuo Nakajima ◽  
Tetsuji Koyama ◽  
Eizo Kakishita ◽  
Kiyoyasu Nagai
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Vascular Endothelial Cells ◽  
Platelet Glycoprotein ◽  
Vascular Endothelial ◽  
Inhibitory Effects
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