A study of cell proliferation in formalin-fixed, wax-embedded bone marrow trephine biopsies using the monoclonal antibody PC10, reactive with proliferating cell nuclear antigen (PCNA)

1992 ◽  
Vol 166 (1) ◽  
pp. 45-52 ◽  
Author(s):  
B. S. Wilkins ◽  
S. Harris ◽  
N. H. Waseem ◽  
D. P. Lane ◽  
D. B. Jones
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Bone Marrow Trephine ◽  
Proliferating Cell ◽  
Formalin Fixed

scholarly journals Proliferating cell nuclear antigen (PCNA) expressed in human leptomeninges.

1996 ◽  
Vol 44 (11) ◽  
pp. 1261-1265 ◽  
Author(s):  
H Funato ◽  
M Yoshimura ◽  
Y Ito ◽  
R Okeda ◽  
Y Ihara
Keyword(s):  
Monoclonal Antibody ◽  
Cell Proliferation ◽  
Smooth Muscle ◽  
Proliferating Cell Nuclear Antigen ◽  
Normal Subjects ◽  
Nuclear Antigen ◽  
Ependymal Cells ◽  
Ki 67 ◽  
Proliferating Cell ◽  
Strong Immunoreactivity

Here we report on the presence of proliferating cell nuclear antigen (PCNA) in human leptomeninges from 35 normal subjects with ages ranging from 57 to 94 years. Strong immunoreactivity with PC10 (a monoclonal antibody to PCNA) was detected in the nuclei of meningothelial cells, smooth muscle cells of leptomeningeal vessels, and ependymal cells. An immunoblot of leptomeningeal homogenate with PC10 showed the presence of a single band at 35 KD, the expected molecular mass of PCNA. Ki-67, another marker for cell proliferation, was undetectable in human leptomeninges. These observations point to isolated PCNA expression in tissue in which cells are not actively proliferating.

scholarly journals Evaluation of proliferating cell nuclear antigen (PCNA) as an endogenous marker of cell proliferation in rat liver: a dual-stain comparison with 5-bromo-2'-deoxyuridine.

1993 ◽  
Vol 41 (1) ◽  
pp. 1-6 ◽  
Author(s):  
K M Connolly ◽  
M S Bogdanffy
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Rat Liver ◽  
Proliferating Cell Nuclear Antigen ◽  
Simultaneous Detection ◽  
S Phase ◽  
Phase Fraction ◽  
Nuclear Antigen ◽  
Proliferating Cell ◽  
Formalin Fixed

Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure that allows the simultaneous detection of the two antigens. The results of this study suggest that although statistically similar indexes for each can be achieved, what has been reported to be the "S-phase fraction" of PCNA-labeled nuclei is significantly different from the population of cells marked by BrdU. The data also suggest that the reason for this difference is that the "S-phase fraction" of PCNA-labeled nuclei is the population of cells in late G1- and early S-phases. BrdU, by comparison, is incorporated into cells only during DNA synthesis. Therefore, although BrdU and PCNA labeling techniques may both be effective for evaluating cell proliferation rates, it must be recognized that labeling indices derived from each are not entirely synonymous. The method presented here for the simultaneous labeling of PCNA and BrdU antigens may have utility in studies of cell cycle perturbations.

scholarly journals Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation.

Gut ◽  
1994 ◽  
Vol 35 (4) ◽  
pp. 530-535 ◽  
Author(s):  
F J Kubben ◽  
A Peeters-Haesevoets ◽  
L G Engels ◽  
C G Baeten ◽  
B Schutte ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferating Cell

Ki-S1 and Proliferating Cell Nuclear Antigen Expression of Bone Marrow Macrophages

1994 ◽  
Vol 91 (3) ◽  
pp. 144-149 ◽  
Author(s):  
B.R. Titius ◽  
J. Thiele ◽  
H. Schaefer ◽  
H. Kreipe ◽  
R. Fischer
Keyword(s):  
Bone Marrow ◽  
Proliferating Cell Nuclear Antigen ◽  
Antigen Expression ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen as the Cell Cycle Sensor for an HLA-Derived Peptide Blocking T Cell Proliferation

2000 ◽  
Vol 164 (12) ◽  
pp. 6188-6192 ◽  
Author(s):  
Xuefeng Ling ◽  
Salar Kamangar ◽  
Michelle L. Boytim ◽  
Zvi Kelman ◽  
Philip Huie ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
T Cell ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
T Cell Proliferation ◽  
Proliferating Cell
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