Histone Deacetylase 4 Promotes Osteosarcoma Cell Proliferation and Invasion by Regulating Expression of Proliferating Cell Nuclear Antigen

Problem To determine if epidermal cell proliferation in colony-raised Fischer 344 rats changes with age and diet. Methods Fischer 344 rats fed ad libitum and calorie-restricted (CR) diets were obtained from the NIA colonies, and young, young adult, and old animals from both groups were used for this study (six in each group). Tissue sections from the dorsal skin (DS) and foot plate (FP) were used for immunohistochemical staining of proliferating cell nuclear antigen (PCNA). The proliferation index (PCNA-I) was computed from counts of stained and total number of keratinocytes. Simultaneous measurements of epidermal thickness were obtained from same sections. Data were analyzed with Excel and SPSS 14.0 software for statistics. Results Two-way analysis of variance (ANOVA) was applied to the data to probe the effect of age, diet, and age-diet interaction. A significant effect of age was noticed in the two parameters i.e., DS PCNA (F 3.96, P .011), FP epidermal width (F 3.37, P .021) and FP PCNA-I (F 9.0, P .000). A significant correlation between DS width and PCNA values was also noted (r 0.5, P .01). Conclusion There is a trend of reduction of PCNA positive cells with increasing age irrespective of thickness of epidermis, and this trend is more apparent in CR rats. Significance This cell proliferation study has implications in relation to CR effect on age-related disease conditions, and biogerontology. Support The study was partially funded by the 2007 Leslie Bernstein grant from AAFPRS foundation.

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Toxicological and Pathological Applications of Proliferating Cell Nuclear Antigen (PCNA), A Novel Endogenous Marker for Cell Proliferation

Critical Reviews in Toxicology ◽

10.3109/10408449309104075 ◽

1993 ◽

Vol 23 (1) ◽

pp. 77-109 ◽

Cited By ~ 119

Author(s):

Daniel R. Dietrich

Keyword(s):

Cell Proliferation ◽

Proliferating Cell Nuclear Antigen ◽

Nuclear Antigen ◽

Proliferating Cell

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Cell Proliferation as Assessed by Expression of Proliferating Cell Nuclear Antigen or the Uptake of Bromodeoxyuridine in a Rabbit Model of Leg-lengthening

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632511 ◽

1996 ◽

Vol 09 (03) ◽

pp. 95-100 ◽

Cited By ~ 2

Author(s):

G. Li ◽

A. H. R. W. Simpson ◽

J. Kenwright ◽

J. T. Triffitt

Keyword(s):

Cell Proliferation ◽

Proliferating Cell Nuclear Antigen ◽

Rabbit Model ◽

Experimental System ◽

Nuclear Antigen ◽

Positive Staining ◽

Leg Lengthening ◽

Staining Index ◽

Proliferating Cell ◽

Distraction Rate

SummaryAn experimental model of leg lengthening has been used to study the cellular responses of the regenerating bone to different rates of distraction. Cell proliferation were assessed by detection of proliferating cell nuclear antigen using a monoclonal antibody, PC10. The technique was verified by comparison with bromodeoxyuridine uptake and subsequent detection with specific antibody (Bu20A). The positive staining index (PSI) was calculated for a variety of tissues and the PC10 PSI was greater than that of Bu20A, as described by the expression: PC10 PSI = 1.6 Bu20A PSI + 12.9, with a correlation coefficient 0.79. The results suggest that PC10 may be used as an alternative marker to assess cell proliferation in rabbit regenerating bone tissue. In addition, the rate of cell proliferation during leg-lengthening was found to reach a maximum at a distraction rate of 0.7 mm/day without further change at higher rates.Cell proliferation was assessed in an experimental system of leg-lengthening by two separate methods. The presence of proliferating cell nuclear antigen or the uptake of bromodeoxyuridine were determined immuno-histochemically. Both methods indicated cell proliferation during leg-lengthening reaches a maximum at a distraction rate of 0.7 mm/day.

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