scholarly journals Hybridomas for production of monoclonal antibodies to human erythropoietin

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 357-364 ◽  
Author(s):  
S Yanagawa ◽  
S Yokoyama ◽  
K Hirade ◽  
R Sasaki ◽  
H Chiba ◽  
...  

Abstract Human urinary erythropoietin has been highly purified by a combination of conventional purification methods and immunoadsorbent columns packed with hybridoma-produced antibodies against contaminants that seemed difficult to separate from erythropoietin by the usual means. By using the partially purified erythropoietin as an antigen, three hybridoma clones have been obtained that secrete monoclonal antibodies against erythropoietin. One of the clones has been quite stable, with a rapid growth rate and high production of antibody. Western blotting technique with monoclonal antibodies revealed occurrence of two species of erythropoietin. The monoclonal antibody will be useful as a probe for the purification of erythropoietin and for further studies of the hormone and its mechanism of action.

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 357-364 ◽  
Author(s):  
S Yanagawa ◽  
S Yokoyama ◽  
K Hirade ◽  
R Sasaki ◽  
H Chiba ◽  
...  

Human urinary erythropoietin has been highly purified by a combination of conventional purification methods and immunoadsorbent columns packed with hybridoma-produced antibodies against contaminants that seemed difficult to separate from erythropoietin by the usual means. By using the partially purified erythropoietin as an antigen, three hybridoma clones have been obtained that secrete monoclonal antibodies against erythropoietin. One of the clones has been quite stable, with a rapid growth rate and high production of antibody. Western blotting technique with monoclonal antibodies revealed occurrence of two species of erythropoietin. The monoclonal antibody will be useful as a probe for the purification of erythropoietin and for further studies of the hormone and its mechanism of action.


2012 ◽  
Vol 15 (1) ◽  
Author(s):  
José Augusto Leitão Drummond ◽  
José Luiz De Andrade Franco ◽  
Daniela De Oliveira

This is a second overview of the Brazilian conservation unit system for mid-2010. It updates author et al, 2009. It examines six dimensions of federal and state protected areas – age, numbers, types of units, absolute and average sizes, distribution by states and biomes, and degree of compliance with CBD-inspired goals. Major findings: (i) the system maintained a rapid growth rate; (ii) national parks and national forests are the most prominent units; (iii) distribution of units by region and biome remains unbalanced; (iv) state units grew remarkably over the last five years; (v) state units are biased towards sustainable use; (vi) sustainable use units grew more than fully protected units; (vii) Amazonia remains the most extensively protected biome; and (viii) quantitative goals of biome protection are closer to being reached. In 2010 Brazil held the fourth position globally in protected areas; it created the largest number of units between 2000 and 2010; it has the largest combined area of protected tropical formations. However, several regions and biomes remain under protected. 


2016 ◽  
Vol 74 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Nikolina Kružić ◽  
Bosiljka Mustać ◽  
Ivan Župan ◽  
Slavica Čolak

Abstract The objective of this review is to present current status of meagre aquaculture in Croatia. Meagre Argyrosomus regius (Asso, 1801) is a fast growing migratory fish species which used to be widespread along the coast of the Adriatic Sea. Today, it is very rare in fisheries catches and is considered highly endangered. Recently, meagre has become an increasingly important species in the Mediterranean aquaculture. In Croatia, meagre is a relatively new cultured species whose farming started after the year 2000. Since the European Commission identified species diversification as a top priority in the framework of the 2020 strategies for Aquaculture, and with recent studies revealing that meagre adapt easily to captivity and maintain a rapid growth rate, this species became an interesting candidate for Croatian as well as Mediterranean aquaculture.


1963 ◽  
Vol 42 (6) ◽  
pp. 1393-1397 ◽  
Author(s):  
R. George Jaap

1948 ◽  
Vol 46 (3) ◽  
pp. 349-349

The Toxicity of Methallyl Chloride and Methyl Bromide towards White RatsBy N. van Tiel, 46, No. 2, p. 217p. 219, column 1, lines 9–11 should read:“The effect of the several gassings upon those rats which survived was studied during and after treatment.”p. 220, column 1, lines 12–16 should read:“This rapid growth rate diminished the differences between the average body weights of the treated and untreated rats.”


1930 ◽  
Vol 2 (4) ◽  
pp. 249-263 ◽  
Author(s):  
A. H. Hutchinson ◽  
Dorothy Newton

An isolated strain of Yeast, derived from Fleischman's preparation, when exposed to monochromatic light responds quite differently to light of different wave-lengths. Slight retardation in growth by red and orange light, stimulation by yellow and green, and increasingly marked retardation in the blue and violet, characterize the effects of the mercury lines of the visible spectrum. There is a sudden transition to stimulation in the near ultra-violet. (λ 3984 Å). Stimulation continues at λ 3650 Å and characterises the region λ 2894 Å to λ 2700 Å, but otherwise the lines employed in this investigation retard growth.Generally the effect either of stimulation or retardation is greater when the control is one of rapid growth rate, but the reverse is the case at the red end of the visible spectrum.


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