Rapid isolation and characterization of bacterial lipopeptides using liquid chromatography and mass spectrometry analysis

PROTEOMICS ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2620-2627 ◽  
Author(s):  
Yan Kwok ◽  
Wang-Chou Sung ◽  
Annie Li-Hsiu Lin ◽  
Hsueh-Hung Liu ◽  
Frieda Ai-Hsiang Chou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Rapid Isolation ◽  
Isolation And Characterization

scholarly journals Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules

2015 ◽  
Author(s):  
Kok-Gan Chan ◽  
Wen-Si Tan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Synthesis Activity

Enterobacter cancerogenus strain M004 genome size is 5.67 Mb. Here, its luxI homologue, designated as ecnI which is ecnI gene (633 bp) was cloned and overexpressed. Its AHL synthesis activity was verified using the high-resolution liquid chromatography-mass spectrometry analysis revealed the production of N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL). The cloning and characterization of luxI homologue of E. cancerogenus strain M004 was firstly reported here.

scholarly journals Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules

2015 ◽  
Author(s):  
Kok-Gan Chan ◽  
Wen-Si Tan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Synthesis Activity

Enterobacter cancerogenus strain M004 genome size is 5.67 Mb. Here, its luxI homologue, designated as ecnI which is ecnI gene (633 bp) was cloned and overexpressed. Its AHL synthesis activity was verified using the high-resolution liquid chromatography-mass spectrometry analysis revealed the production of N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL). The cloning and characterization of luxI homologue of E. cancerogenus strain M004 was firstly reported here.

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