Secretome profiling of cytokines and growth factors reveals that neuro-glial differentiation is associated with the down-regulation of Chemokine Ligand 2 (MCP-1/CCL2) in amniotic fluid derived-mesenchymal progenitor cells

PROTEOMICS ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 674-688 ◽  
Author(s):  
Marco Miceli ◽  
Carmela Dell'Aversana ◽  
Rosita Russo ◽  
Camilla Rega ◽  
Lorenzo Cupelli ◽  
...  
Keyword(s):  
Growth Factors ◽  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Glial Differentiation ◽  
Down Regulation ◽  
Chemokine Ligand ◽  
Chemokine Ligand 2 ◽  
Secretome Profiling

A targeted secretome profiling by multiplexed immunoassay revealed that secreted chemokine ligand 2 (MCP-1/CCL2) affects neural differentiation in mesencephalic neural progenitor cells

PROTEOMICS ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 714-724 ◽  
Author(s):  
Luca Colucci-D'Amato ◽  
Anna Emilia Cicatiello ◽  
Mafalda Giovanna Reccia ◽  
Floriana Volpicelli ◽  
Valeria Severino ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Differentiation ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Chemokine Ligand ◽  
Multiplexed Immunoassay ◽  
Chemokine Ligand 2 ◽  
Secretome Profiling

scholarly journals Transplantation of Cardiac Mesenchymal Progenitor Cell Sheets for Myocardial Vascularization after an Infarction (Experimental Study)

2018 ◽  
Vol 14 (6) ◽  
pp. 28-40 ◽  
Author(s):  
Konstantin V. Dergilev ◽  
Zoya I. Tsokolaeva ◽  
Ivan A. Ryzhkov ◽  
Elena V. Parfenova
Keyword(s):  
Myocardial Infarction ◽  
Growth Factors ◽  
Progenitor Cells ◽  
Connexin 43 ◽  
Solid Phase ◽  
Reference Group ◽  
Right Atrial ◽  
Rational Approach ◽  
Mesenchymal Progenitor Cells ◽  
Cell Sheets

Purpose. To develop a method of producing tissue-engineered constructs (TECs) on the basis of resident mesenchymal progenitor cells (MPC) of the human heart and to assess the effect of TECs transplantation on regenerative processes in the heart using a model of myocardial infarction in rats.Materials and methods. Human resident MPCs were isolated from the right atrial auricle of CAD patients. A similar protocol was used to obtain MPCs from Wistar rats. The MPC immunophenotype was determined by cytofluorometry. Corresponding TECs were obtained on the basis of MPC sheets of human and rats' hearts. Myocardial infarction in rats was induced by ligation of the anterior descending coronary artery followed by TEC transplantation. Euthanasia was performed 30 days after the transplantation. Histological examination of the implant and vascularization cells, morphometric analysis, tracking of the MPC differentiation ability, determination of the content of growth factors by solid-phase ELISA were carried out. Statistical evaluation of the significance of differences was performed using the Statistica 8.0 software package.Results. The analysis of the obtained cell constructs showed that they consisted of several layers of cells interacting with each other by means of connexin 43 and were characterized by good cell viability as a part TECs. The number of vessels in the peri-infarction area under the transplant from the MPC was significantly higher than that in the reference group with signs of differentiation of cardiac MPCs transplanted into endothelial vascular cells.The increased vascularization was combined with an increase in the area of viable myocardial sites and a decrease in LV cavity dilation. Analysis of the cardiac MPC secretion products showed that they produce the most important growth factors and cytokines that regulate angiogenesis and migration of stem cells.Conclusion. The strategy of using epicardial TEC transplantation based on MPC sheets seems to be a rational approach for effective delivery of viable stem/progenitor cells to the damaged myocardium. The use of TEC helps to reduce or temporarily eliminate the effect of factors that contribute to progressive heart dysfunction by local paracrine exposure and activation of the revascularization processes in the affected zone.

scholarly journals In vitro and in vivo properties of distinct populations of amniotic fluid mesenchymal progenitor cells

2011 ◽  
Vol 15 (9) ◽  
pp. 1896-1913 ◽  
Author(s):  
Maria G. Roubelakis ◽  
Vasiliki Bitsika ◽  
Dimitra Zagoura ◽  
Ourania Trohatou ◽  
Kalliopi I. Pappa ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells

scholarly journals Dynamic mechanical loading and growth factors influence chondrogenesis of induced pluripotent mesenchymal progenitor cells in a cartilage-mimetic hydrogel

2019 ◽  
Vol 7 (12) ◽  
pp. 5388-5403
Author(s):  
Elizabeth A. Aisenbrey ◽  
Ganna Bilousova ◽  
Karin Payne ◽  
Stephanie J. Bryant
Keyword(s):  
Growth Factors ◽  
Progenitor Cells ◽  
Mechanical Loading ◽  
Mesenchymal Progenitor Cells ◽  
Dynamic Mechanical ◽  
Exogenous Growth ◽  
Induced Pluripotent ◽  
Exogenous Growth Factors ◽  
Biophysical Cues

The chondrogenesis of iPSC-mesenchymal progenitor cells in a cartilage mimetic hydrogel is dependent on the combinatory effects of biophysical cues and exogenous growth factors.

scholarly journals A Comparative Study on Culture Conditions and Routine Expansion of Amniotic Fluid-Derived Mesenchymal Progenitor Cells

2013 ◽  
Vol 34 (4) ◽  
pp. 225-235 ◽  
Author(s):  
L. Gucciardo ◽  
N. Ochsenbein-Kölble ◽  
Y. Ozog ◽  
G. Verbist ◽  
V. Van Duppen ◽  
...  
Keyword(s):  
Comparative Study ◽  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Culture Conditions ◽  
Mesenchymal Progenitor Cells

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties

2007 ◽  
Vol 313 (5) ◽  
pp. 1008-1023 ◽  
Author(s):  
Mitsutaka Shiota ◽  
Toshio Heike ◽  
Munetada Haruyama ◽  
Shiro Baba ◽  
Atsunori Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Isolation And Characterization
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