scholarly journals Pressure Cycling Technology Assisted Mass Spectrometric Quantification of Gingival Tissue Reveals Proteome Dynamics during the Initiation and Progression of Inflammatory Periodontal Disease

PROTEOMICS ◽  
2020 ◽  
Vol 20 (3-4) ◽  
pp. 1900253 ◽  
Author(s):  
Kai Bao ◽  
Xiaofei Li ◽  
Tetsuhiro Kajikawa ◽  
Abe Toshiharu ◽  
Nathalie Selevsek ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Mass Spectrometric ◽  
Gingival Tissue ◽  
Pressure Cycling ◽  
Pressure Cycling Technology

scholarly journals Morphometric analysis of collagen and inflammatory cells in periodontal disease

2015 ◽  
Vol 72 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Ranko Golijanin ◽  
Bojan Kujundzic ◽  
Zoran Milosavljevic ◽  
Dragan Milovanovic ◽  
Zlatibor Andjelkovic ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Morphometric Analysis ◽  
Plasma Cells ◽  
Relative Proportion ◽  
Inflammatory Cells ◽  
Test System ◽  
Clinical Staging ◽  
Gingival Tissue ◽  
Tissue Destruction ◽  
Immunochemical Method

Background/Aim. Periodontal disease affects gingival tissue and supporting apparatus of the teeth leading to its decay. The aim of this study was to highlight and precisely determine histological changes in the gum tissue. Methods. Gingival biopsy samples from 53 healthy and parodontopathy-affected patients were used. Clinical staging of the disease was performed. Tissue specimens were fixed and routinely processed. Sections, 5 ?m thin, were stained with hematoxylin and eosin, histochemical Van-Gieson for the collagen content, Spicer method for mast-cells and immunochemical method with anti-CD68 and anti-CD38 for the labelling of the macrophages and plasma-cells. Morphometric analysis was performed by a M42 test system. Results. While the disease advanced, collagen and fibroblast volume density decreased almost twice in the severe cases compared to the control ones, but a significant variation was observed within the investigated groups. The mast-cell number increased nearly two times, while the macrophage content was up to three times higher in severe parodontopathy than in healthy gingival tissue. However, the relative proportion of these cells stayed around 6% in all cases. Plasma-cells had the most prominent increase in the number (over 8 times) compared to the control, but again, a variation within investigated groups was very high. Conclusion. Gingival tissue destruction caused by inflammatory process leads to significant changes in collagen density and population of resident connective tissue cells. Although inflammatory cells dominated with the disease advancing, a high variation within the same investigated groups suggests fluctuation of the pathological process. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/VSP1704391E">10.2298/VSP1704391E</a><u></b></font>

scholarly journals The occurrence of Entamoeba gingivalis among patients with periodontal disease

2016 ◽  
Vol 29 (2) ◽  
pp. 86-89 ◽  
Author(s):  
Joanna Luszczak ◽  
Michal Bartosik ◽  
Jolanta Rzymowska ◽  
Agnieszka Sochaczewska-Dolecka ◽  
Ewa Tomaszek ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Age Groups ◽  
Healthy People ◽  
Gingival Tissue ◽  
Pathological Changes ◽  
Oral Diseases

AbstractAccording to some studies, the Entamoeba gingivalis colonizing the gingival tissue is an important agent in bringing about periodontitis. Other studies, however, deem it an opportunist that is able to survive in the medium induced by periodontal disease. The aim of this study was to investigate the prevalence of Entamoeba gingivalis infection in patients from the Department of Periodontology, and compare this population with that of healthy people, so as to analyze the relationships between infection and patient sex and age. The result of this work is that in both groups, a correlation (p = 0,19) has been noted between the occurrence of amoebae and other diseases in the oral cavity. Indeed, 81,4% of all patients with some periodontal disease showed the presence of amoeba. Among those who are not afflicted with oral diseases, the presence of amoeba was indicated in 62,5% of the total. In addition, a correlation between the person's age and the presence of protozoa (p = 0,15) was strongly marked among women (p = 0,19). In the three age groups of women in this study (40-49, 60-69, and above 80 years), we observed a 100% presence of protozoa.Our study leads us to the conclusion that infections with Entamoeba gingivalis should be regarded as an factor that is associated with the pathological changes occurring in patients with periodontal diseases.

scholarly journals Salivary antioxidants and periodontal disease status

2002 ◽  
Vol 61 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Dean V. Sculley ◽  
Simon C. Langley-Evans
Keyword(s):  
Periodontal Disease ◽  
Antioxidant Status ◽  
Alveolar Bone ◽  
Disease Status ◽  
Gingival Tissue ◽  
Root Area ◽  
Nutritional Strategy ◽  
Dietary Antioxidant ◽  
Factor Α ◽  
High Degree

Periodontal disease is a common chronic adult condition. The bacteriumPorphyromonas gingivalishas been implicated in the aetiology of this disease, which causes destruction of the connective tissue and bone around the root area of the tooth. It has been observed that invadingP. gingivalisbacteria trigger the release of cytokines such as interleukin 8 and tumour necrosis factor α, leading to elevated numbers and activity of polymorphonucleocytes (PMN). As a result of stimulation by bacterial antigens, PMN produce the reactive oxygen species (ROS) superoxide via the respiratory burst as part of the host response to infection. Patients with periodontal disease display increased PMN number and activity. It has been suggested that this proliferation results in a high degree of ROS release, culminating in heightened oxidative damage to gingival tissue, periodontal ligament and alveolar bone. Antioxidant constituents in plasma have been well-documented, being chiefly ascorbate, albumin and urate, and these are known to display sensitivity to dietary antioxidant intakes. The concentration of antioxidants in saliva does not appear to mirror those of plasma. The extent of dietary influence upon salivary antioxidant status is unclear. Urate is the predominant salivary antioxidant, with albumin and ascorbate providing minor contributions. Previous research has found reduced salivary antioxidant activity in patients suffering from periodontal disease. An improved understanding of the role antioxidants play in periodontitis, and the influence of nutrition on antioxidant status, may lead to a possible nutritional strategy for the treatment of periodontal disease.

Accelerated Lysis and Proteolytic Digestion of Biopsy-Level Fresh-Frozen and FFPE Tissue Samples Using Pressure Cycling Technology

2020 ◽  
Vol 19 (5) ◽  
pp. 1982-1990 ◽  
Author(s):  
Huanhuan Gao ◽  
Fangfei Zhang ◽  
Shuang Liang ◽  
Qiushi Zhang ◽  
Mengge Lyu ◽  
...  
Keyword(s):  
Ffpe Tissue ◽  
Proteolytic Digestion ◽  
Tissue Samples ◽  
Pressure Cycling ◽  
Fresh Frozen ◽  
Pressure Cycling Technology

Immunoregulatory Aspects of Periodontal Disease

1988 ◽  
Vol 2 (2) ◽  
pp. 328-333 ◽  
Author(s):  
M.A. Taubman ◽  
E.D. Stoufi ◽  
G.J. Seymour ◽  
D.J. Smith ◽  
J.L. Ebersole
Keyword(s):  
Periodontal Disease ◽  
Normal Tissue ◽  
Cell Subset ◽  
Gingival Tissue ◽  
Healthy Human ◽  
Flow Cytofluorometry ◽  
Immunoglobulin Synthesis ◽  
Periodontal Tissues ◽  
Gingival Cells ◽  
Tissue Cells

This manuscript reviews our studies of the composition and functional capabilities of gingival tissue lymphocytes from patients with periodontal disease. The emphasis has been on phenotyping the local lymphoid infiltration in gingival and periodontal disease. The preparation and phenotypic analyses of cells recovered from diseased and healthy human periodontal tissues indicated that T-cell subset ratios from diseased tissue were significantly decreased compared with peripheral blood or normal tissue ratios. These reductions were verified in a second study we performed using two-color immunofluorescence analyzed by flow cytofluorometry. Local variations in the CD4 + cell population were also found in diseased tissue cells when these were compared with normal tissue cells. The relative percentage of CD4+ cells labeled with anti-helper inducer (4B4) or anti-suppressor inducer (2H4) monoclonal antibodies was increased above that of normal tissue cells. Functional studies of immunoglobulin production by gingival cells from adult periodontitis tissues showed two discrete patterns of synthesis and also suppression of immunoglobulin synthesis after addition of mitogen to the cultures. Removal of macrophages also drastically reduced immunoglobulin synthesis by gingival cells. These results indicate that there is an abundance of suppressor T-cells in diseased tissue and that functional suppression is demonstrated by lymphocytes from periodontal disease tissue. The findings of these investigations have suggested potentially important roles for immune regulation in periodontal disease.

Pressure cycling technology (PCT) reduces effects of inhibitors of the PCR

2012 ◽  
Vol 127 (2) ◽  
pp. 321-333 ◽  
Author(s):  
Pamela L. Marshall ◽  
Jonathan L. King ◽  
Nathan P. Lawrence ◽  
Alexander Lazarev ◽  
Vera S. Gross ◽  
...  
Keyword(s):  
Pressure Cycling ◽  
Pressure Cycling Technology

scholarly journals Periodontal evaluation using a non-invasive imaging method (ultrasonography)

2019 ◽  
Vol 92 ◽  
pp. S20-S32 ◽  
Author(s):  
Radu Chifor ◽  
Alexandru Florin Badea ◽  
Ioana Chifor ◽  
Delia-Alexandrina Mitrea ◽  
Maria Crisan ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Alveolar Bone ◽  
Adult Population ◽  
World Health ◽  
Gingival Tissue ◽  
Imaging Method ◽  
Gingival Inflammation ◽  
Extensive Experience ◽  
Reliable Technique ◽  
Non Invasive

The periodontal disease and gingival bleeding are highly prevalent in the adult population worldwide. The World Health Organization (WHO) data shows that 90–100% of the 34-year-old adults present gingival inflammation. Therefore, an investigation method is required to allow the assessment of the periodontal disease as well as the monitoring of the evolution of the gingival inflammation after periodontal treatments. Non-invasive and operator-independent methods for periodontal examination are necessary for diagnosing and monitoring the periodontal disease. The periodontal ultrasonography is a reliable technique for visualizing the anatomical elements which are necessary to diagnose the periodontal status. Using this imaging technique the dentino-enamel junction, the cortical bone, the radicular surface from the crown to the alveolar bone, the gingival tissue can be seen without interfering with those elements during the examination. Also, calculus visualization is possible before and after scaling in order to evaluate the quality of the treatment. Using 2D ultrasonography is not feasible in dental practice as it requires extensive experience and is also time consuming. The reproducibility of the 2D slices is very difficult in order to have the possibility to compare different investigations efficiently. 3D reconstructions of the periodontal tissue can be a very good alternative to eliminate the operator dependence. Ultrasonography allows the practitioner to visualize the anatomic elements involved in making a periodontal diagnosis. It also allows tracking of subsequent changes. This method is not commonly used for periodontal examination and further studies are required. Previous studies show that ultrasonography can be a reliable non-invasive method to diagnose and monitor the periodontal disease.

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