scholarly journals Computational Structure Prediction Provides a Plausible Mechanism for Electron Transfer by the Outer Membrane Protein Cyc2 from Acidithiobacillus ferrooxidans

2021 ◽  
Author(s):  
Virginia Jiang ◽  
Sagar D. Khare ◽  
Scott Banta
Keyword(s):  
Electron Transfer ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Acidithiobacillus Ferrooxidans ◽  
Structure Prediction ◽  
Computational Structure ◽  
Plausible Mechanism

scholarly journals Computational Structure Prediction Provides a Plausible Mechanism for Electron Transfer by the Outer Membrane Protein Cyc2 from Acidithiobacillus ferrooxidans

2021 ◽  
Author(s):  
Virginia Jiang ◽  
Sagar D Khare ◽  
Scott Banta
Keyword(s):  
Electron Transfer ◽  
Outer Membrane ◽  
Acidithiobacillus Ferrooxidans ◽  
Structure Prediction ◽  
Structural Model ◽  
Comparative Modeling ◽  
Transport Chain ◽  
Associated Proteins ◽  
Periplasmic Proteins ◽  
Plausible Mechanism

Cyc2 is the key protein in the outer membrane of Acidithiobacillus ferrooxidans that mediates electron transfer between extracellular inorganic iron and the intracellular central metabolism. This cytochrome c is specific for iron and interacts with periplasmic proteins to complete a reversible electron transport chain. A structure of Cyc2 has not yet been characterized experimentally. Here we describe a structural model of Cyc2, and associated proteins, to highlight a plausible mechanism for the ferrous iron electron transfer chain. A comparative modeling protocol specific for trans membrane beta barrel (TMBB) proteins in acidophilic conditions (pH ~2) was applied to the primary sequence of Cyc2. The proposed structure has three main regimes: extracellular loops exposed to low-pH conditions, a TMBB, and a N-terminal cytochrome-like region within the periplasmic space. The Cyc2 model was further refined by identifying likely iron and heme docking sites. This represents the first computational model of Cyc2 that accounts for the membrane microenvironment and the acidity in the extracellular matrix. This approach can be used to model other TMBBs which can be critical for chemolithotrophic microbial growth.

scholarly journals Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans

Extremophiles ◽  
2011 ◽  
Vol 15 (3) ◽  
pp. 403-410 ◽  
Author(s):  
Mohammed Abul Manchur ◽  
Mei Kikumoto ◽  
Tadayoshi Kanao ◽  
Jun Takada ◽  
Kazuo Kamimura
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Acidithiobacillus Ferrooxidans

scholarly journals Structure Prediction and Simulations of the Major Outer Membrane Protein of Chlamydia

2011 ◽  
Vol 100 (3) ◽  
pp. 321a
Author(s):  
Muhammad S. Noon ◽  
Joseph B. Lim ◽  
Alexander D. MacKerell ◽  
Jeffery B. Klauda
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Structure Prediction ◽  
Major Outer Membrane Protein

scholarly journals The High-Molecular-Weight Cytochrome c Cyc2 of Acidithiobacillus ferrooxidans Is an Outer Membrane Protein

2002 ◽  
Vol 184 (5) ◽  
pp. 1502-1502
Author(s):  
Andrés Yarzábal ◽  
Gaël Brasseur ◽  
Jeanine Ratouchniak ◽  
Karen Lund ◽  
Danielle Lemesle-Meunier ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cytochrome C ◽  
Membrane Protein ◽  
Outer Membrane ◽  
High Molecular Weight ◽  
Outer Membrane Protein ◽  
Acidithiobacillus Ferrooxidans

Electron Transfer Activity of Mitochondrial Outer Membrane Protein MitoNEET

2016 ◽  
Vol 100 ◽  
pp. S183
Author(s):  
Yiming Wang ◽  
Aaron P. Landry ◽  
Huangen Ding
Keyword(s):  
Electron Transfer ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Mitochondrial Outer Membrane ◽  
Transfer Activity

Electron transfer kinetics of the mitochondrial outer membrane protein mitoNEET

2018 ◽  
Vol 121 ◽  
pp. 98-104 ◽  
Author(s):  
Xiaokang Li ◽  
Yiming Wang ◽  
Guoqiang Tan ◽  
Jianxin Lyu ◽  
Huangen Ding
Keyword(s):  
Electron Transfer ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Mitochondrial Outer Membrane ◽  
Electron Transfer Kinetics ◽  
Kinetics Of
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