Development of a female attractant for the click beetle pest Agriotes brevis

2013 ◽  
Vol 70 (4) ◽  
pp. 610-614 ◽  
Author(s):  
József Vuts ◽  
Lorenzo Furlan ◽  
Éva Bálintné Csonka ◽  
Christine M Woodcock ◽  
John C Caulfield ◽  
...  
Keyword(s):  
Click Beetle ◽  
Female Attractant

scholarly journals Estimation of abundance and dispersal distance of the sugarcane click beetle Melanotus sakishimensis Ohira (Coleoptera: Elateridae) on Kurima Island, Okinawa, by mark-recapture experiments

2008 ◽  
Vol 43 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Norio Arakaki ◽  
Atsushi Nagayama ◽  
Aya Kobayashi ◽  
Kazuhiko Tarora ◽  
Mitsunobu Kishita ◽  
...  
Keyword(s):  
Dispersal Distance ◽  
Mark Recapture ◽  
Click Beetle

scholarly journals A novel thermostable beetle luciferase based cytotoxicity assay

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sunju Choi ◽  
Hittu Matta ◽  
Ramakrishnan Gopalakrishnan ◽  
Venkatesh Natarajan ◽  
Songjie Gong ◽  
...  
Keyword(s):  
Membrane Integrity ◽  
Cytotoxicity Assay ◽  
Luciferase Assay ◽  
Target Cells ◽  
Over Expression ◽  
Click Beetle ◽  
In Vivo Bioluminescence Imaging ◽  
Cost Efficient ◽  
Dying Cells

AbstractCytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the development of a new cytotoxicity assay termed ‘Matador-Glo assay’ which takes advantage of a thermostable variant of Click Beetle Luciferase (Luc146-1H2). Matador-Glo assay utilizes Luc146-1H2 and D-luciferin as the luciferase-substrate pair for luminescence detection. The assay involves ectopic over-expression of Luc146-1H2 in the cytosol of target cells of interest. Upon damage to the membrane integrity, the Luc146-1H2 is either released from the dead and dying cells or its activity is preferentially measured in dead and dying cells. We demonstrate that this assay is simple, fast, specific, sensitive, cost-efficient, and not labor-intensive. We further demonstrate that the Matador-Glo assay can be combined with the marine luciferase-based Matador assay to develop a dual luciferase assay for cell death detection. Finally, we demonstrate that the Luc146-1H2 expressing target cells can also be used for in vivo bioluminescence imaging applications.

scholarly journals Comparison of different pheromone traps for monitoring of click beetle Agriotes ustulatus (Coleoptera Elateridae)

2017 ◽  
pp. 155-159
Author(s):  
Tímea Szalárdi ◽  
Katalin Szentannai ◽  
Antal Nagy
Keyword(s):  
Sex Pheromone ◽  
Pheromone Traps ◽  
Preliminary Results ◽  
Click Beetle ◽  
The Way

The effectiveness of two trap types (YATLORf and VARb3 with CSALOMON® sex pheromone bait) for monitoring click beetle Agriotes ustu latus was compared near Karcag (East Hungary) in 2016. Additionally effectiveness of YATLORf traps placed on ground and placed on a 25 cm high mound was also compared. Contrary to our expectations traps caught very few individuals thus our study could provide only preliminary results. Between traps and methods there were not significant differences but YATLORf traps placed on mounds caught more individuals than others. The swarming imagos of A. ustulatus were also detected two weeks earlier in 2016 than as usual. To prove the effect of the way of usage on the effectiveness of YATLORf traps new studies should be made.

scholarly journals Taxonomic notes, a new species, and a key to Indian species of the click beetle genus Cryptalaus Ȏhira, 1967 (Coleoptera: Elateridae: Agrypninae)

2021 ◽  
Vol 13 (13) ◽  
pp. 19985-19999
Author(s):  
Harshad Parekar ◽  
Amol Patwardhan
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Indian Species ◽  
Male And Female ◽  
Median Ridge ◽  
Click Beetle ◽  
A New Species

Cryptalaus alveolatus sp. nov. is described and illustrated from India. The species has unique morphological characters: conspicuous longitudinal median ridge on pronotum, elytral interstria II more depressed than interstriae I and III. The Indian fauna of the genus Cryptalaus Ȏhira, 1967 is updated and represented by seven species: C. alveolatus sp. nov., C. assamensis (Schwarz, 1902) comb. nov., C. eryx (Candèze, 1874), C. lynceus (Candèze, 1874), C. nodulosus (Waterhouse, 1877) comb. nov., C. sculptus (Westwood, 1848) and C. sordidus (Westwood, 1848). Genitalia and terminalia of male and female C. sordidus are also illustrated. A key to the Indian species of Cryptalaus is given.

scholarly journals Firefly genomes illuminate parallel origins of bioluminescence in beetles

2017 ◽  
Author(s):  
Timothy R. Fallon ◽  
Sarah E. Lower ◽  
Ching-Ho Chang ◽  
Manabu Bessho-Uehara ◽  
Gavin J. Martin ◽  
...  
Keyword(s):  
Firefly Luciferase ◽  
Chemical Defenses ◽  
Photinus Pyralis ◽  
Click Beetles ◽  
Origin And Evolution ◽  
The North ◽  
Click Beetle ◽  
Light Organs ◽  
The Caribbean ◽  
Shed Light

AbstractFireflies and their fascinating luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin of their bioluminescence remains unclear. To shed light on this long-standing question, we sequenced the genomes of two firefly species that diverged over 100 million-years-ago: the North AmericanPhotinus pyralisand JapaneseAquatica lateralis.We also sequenced the genome of a related click-beetle, the CaribbeanIgnelater luminosus,with bioluminescent biochemistry near-identical to fireflies, but anatomically unique light organs, suggesting the intriguing but contentious hypothesis of parallel gains of bioluminescence. Our analyses support two independent gains of bioluminescence between fireflies and click-beetles, and provide new insights into the genes, chemical defenses, and symbionts that evolved alongside their luminous lifestyle.One Sentence Summary:Comparative analyses of the first linkage-group-resolution genomes of fireflies and related bioluminescent beetles address long-standing questions of the origin and evolution of bioluminescence and its associated traits.

scholarly journals The phytosanitary status of sunflower crops оf north-eastern Forest-Steppe of Ukraine

2019 ◽  
pp. 3-7
Author(s):  
V.M. Demenko ◽  
O.L. Golinach ◽  
V.A. Vlasenko
Keyword(s):  
Sharp Increase ◽  
Crop Rotations ◽  
Forest Steppe ◽  
Economic Threshold ◽  
Soil Pests ◽  
Darkling Beetle ◽  
Melolontha Melolontha ◽  
Click Beetle ◽  
North Eastern ◽  
Brachycaudus Helichrysi

The high economic efficiency of sunflower growing contributed to a sharp increase in the sunflower planting acreage in Sumy region. The increase of cultivated areas under sunflower resulted in an oversaturation of crop rotations with this crop. The study of the phytosanitary status of sunflower crops was carried out in the basic farms of the phytosanitary security department of the Main Office of State Consumer Service (Derzhprodsluzhba) in Sumy region. The research methodology was commonly accepted. The main pests of sunflower crops were grey beet weevil (Tanymecus palliates Fabr.), larvae of common click beetle (Agriotes sputator L.), darkling beetle (Opatrum sabulosum L.), larvae of the western may beetle (Melolontha melolontha L.), leafcurl plum aphid (Brachycaudus helichrysi Kalt). The sunflower seedlings were damaged grey beet weevil, darkling beetle. The most widespread soil pests were the larvae of the western may beetle and larvae of common click beetle. Leafcurl plum aphid populated sunflower crops with 6‒8 pairs of true leaves. It continued to spread across the field during the inflorescence stage and the stage of initial blossom. The highest pest colonization was observed at the edge of the field in 2015, 2017 and accounted for 16 % of the plants. In the middle of the field, the aphid colonization was lower than at the edge. During the years of research, the economic threshold of sunflower pest harmfulness was exceeded only in some years. Sunflower damage by grey weevil beet, larvae of common click beetle, darkling beetle, larvae of the western may beetle was weak, and their number was insignificant. The increase of sunflower acreage did not lead to a significant growth of pest number, the exceeding of economic threshold of their harmfulness.

Sign in / Sign up

Export Citation Format

Share Document