Genetic Variability in Growth Characteristics among Different Clones of Eucalyptus tereticornis Sm.

The present investigation was carried out at the College of Forestry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India during August, 2018 to June, 2019 to study the genetic variability in growth characteristics among different clones of Eucalyptus tereticornis. Different clones of Eucalyptus tereticornis Sm. were planted in RCBD, with 4 replications revealed significant variations among all eight treatments (clones) with respect to 9 different characters. Based on the mean performance, treatment-1 (clone-526) showed maximum value for characters like biomass (1124.17), plant height (247.9 cm), collar diameter (23.25 mm), and a number of leaves plant-1 (463.25 number). Similarly, the maximum value was observed in treatment-8 (clone-136) for traits like leaf area (42.70 cm2), leaf length (15 cm), and leaf width (5.75 cm). The highest leaf length to leaf width ratio (3.57) and lowest number of branches plant-1 (18.75 number) were found in treatment-2 (clone-288). All characters had exhibited higher genotypic variance than an environmental variance. Similarly, the genetic coefficient of variation in the case of all variables was also found greater than an environmental coefficient of variation. Heritability was found maximum in plant height (87.35%) and all other characters also showed high heritability. Genetic advance as % of mean was found maximum in biomass (71.15%). Based on the overall mean performance of growth characters, Treatment-1 (Clone-526) was found as a superior clone with respect to the most important character biomass for the test locality. High GCV, heritability, and GAM value for biomass indicated that the character would respond to selection for the improvement program.

Estimation of leaf nitrogen content from nondestructive methods in Eucalyptus tereticornis and Eucalyptus saligna plantations

The determination of leaf nitrogen content (LNC) by indirect methods is essential for silvicultural management of forest crops. The application of photography or rapid measurement equipment, such as chlorophyll index (soil-plant analysis development-SPAD), is increasingly used due to its low-cost, ease of estimation and accuracy. Therefore, the aim of this study was to estimate foliar nitrogen content from nondestructive methods in plantations of Eucalyptus tereticornis and Eucalyptus saligna using three urea treatments (120 kg N ha-1, 240 kg N ha-1 and a control treatment without urea). For each treatment, 10 trees were selected, including four for the validation of the equations. The LNC was directly evaluated for color with the CIEL*a*b* model, photographic measurement with the RGB model, SPAD measurement and destructive estimation of nitrogen in leaves. The results showed negative relationships with the L* (luminosity) and b* (trend from yellow to green) indices, while the a* (red to green trend) index was discarded, with SPAD positive relationships were found with LNC and RGB space. In the R and B indices, the greatest negative relationships were found. It was determined that the multivariate equation Y=a+b1x1+b2x2+…+bnxn can be used for this type of study. It was also determined that the LNC=0.389+0.026SPAD model was the optimum for E. tereticornis and the LNC=3.826-0.001R-0.10B equation was the optimum for E. saligna.

Ursolic Acid Lactone Obtained from Eucalyptus tereticornis Increases Glucose Uptake and Reduces Inflammatory Activity and Intracellular Neutral Fat: An In Vitro Study

Obesity has a strong relationship to insulin resistance and diabetes mellitus, a chronic metabolic disease that alters many physiological functions. Naturally derived drugs have aroused great interest in treating obesity, and triterpenoids are natural compounds with multiple biological activities and antidiabetic mechanisms. Here, we evaluated the bioactivity of ursolic acid lactone (UAL), a lesser-known triterpenoid, obtained from Eucalyptus tereticornis. We used different cell lines to show for the first time that this molecule exhibits anti-inflammatory properties in a macrophage model, increases glucose uptake in insulin-resistant muscle cells, and reduces triglyceride content in hepatocytes and adipocytes. In 3T3-L1 adipocytes, UAL inhibited the expression of genes involved in adipogenesis and lipogenesis, enhanced the expression of genes involved in fat oxidation, and increased AMP-activated protein kinase phosphorylation. The range of biological activities demonstrated in vitro indicates that UAL is a promising molecule for fighting diabetes.

Eucalyptus tereticornis (forest red gum).

Eucalyptus tereticornis is a versatile, fast growing and widely planted tree species. It has been especially successful in areas with summer rainfall followed by a moderate to severe dry season, although fast-growing provenances adapted to a winter rainfall are also available from its extensive natural distribution. The species is marginally less drought tolerant than its close relative E. camaldulensis but appears to be less susceptible than this species to leaf pathogens and stem cankers when planted in areas of seasonally-high humidity. Provenances have been identified from the southern, temperate part of the species distribution that are moderately frost and salt tolerant. Eucalyptus tereticornis is proving a valuable partner in various hydrid combinations with other fast growing eucalypts, including E. grandis and E. urophylla. The wood is used for a wide range of purposes including fuel, charcoal, paper, poles, posts, mining timber, hardboard and particleboard while the tree is used for shelterbelts, shade and in apiculture.