scholarly journals Genetic variation in target-site resistance to pyrethroids and pirimicarb in Tunisian populations of the peach potato aphid,Myzus persicae(Sulzer) (Hemiptera: Aphididae)

2016 ◽  
Vol 72 (12) ◽  
pp. 2313-2320 ◽  
Author(s):  
Kamel Charaabi ◽  
Sonia Boukhris-Bouhachem ◽  
Mohamed Makni ◽  
Brian Fenton ◽  
Ian Denholm
Keyword(s):  
Genetic Variation ◽  
Myzus Persicae ◽  
Target Site ◽  
Potato Aphid ◽  
Target Site Resistance ◽  
Peach Potato Aphid

scholarly journals The evolution of insecticide resistance in the peach–potato aphid, Myzus persicae

1998 ◽  
Vol 353 (1376) ◽  
pp. 1677-1684 ◽  
Author(s):  
A. L. Devonshire ◽  
L. M. Field ◽  
S. P. Foster ◽  
G. D. Moores ◽  
M. S. Williamson ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Myzus Persicae ◽  
Temporal Dynamics ◽  
Diagnostic Methods ◽  
Strong Linkage Disequilibrium ◽  
Potato Aphid ◽  
Wide Range ◽  
Target Site Resistance ◽  
External Stimuli ◽  
Peach Potato Aphid

The peach–potato aphid Myzus persicae (Sulzer) can resist a wide range of insecticides, but until recently (1990) the only mechanism identified was the increased production of carboxylesterases (E4 or FE4), which cause enhanced degradation and sequestration of insecticidal esters. We have now identified two forms of target–site resistance involving changes in the acetylcholinesterase ( AChE ) and sodium channel ( kdr ) genes. Biochemical and DNA diagnostic methods can be used to identify all three mechanisms in individual aphids, and thereby establish their spatial distributions and temporal dynamics. Amplified genes underlie the increased production of esterases but their expression is modulated by DNA methylation. Amplification of the E4 gene is in strong linkage disequilibrium with the kdr mechanism. This may reflect strong insecticidal selection favouring aphids with multiple mechanisms, tight chromosomal linkage and/or the prominence of parthenogenesis in many M. persicae populations. The decreased fitness of resistant aphids under winter conditions may be a consequence of the altered sodium–channel gene affecting behaviour and/or the perception of external stimuli.

Intensification of insecticide resistance in UK field populations of the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) in 1996

1998 ◽  
Vol 88 (2) ◽  
pp. 127-130 ◽  
Author(s):  
S.P. Foster ◽  
I. Denholm ◽  
Z.K. Harling ◽  
G.D. Moores ◽  
A.L. Devonshire
Keyword(s):  
Insecticide Resistance ◽  
Myzus Persicae ◽  
Resistance Mechanism ◽  
The Novel ◽  
Potato Aphid ◽  
Aphid Control ◽  
Target Site Resistance ◽  
The Uk ◽  
First Time ◽  
Peach Potato Aphid

AbstractThe well-established carboxylesterase-based resistance to insecticides in Myzus persicae Sulzer has recently been accentuated by the appearance of aphids with a modified acetylcholinesterase (MACE) insensitive to pirimicarb and the novel aphicide, triazamate. This target site resistance mechanism was found in M. persicae from crops in the UK for the first time in 1996, together with especially large proportions of aphids with R2 and R3 levels of carboxylesterases, a combination that was associated with serious insecticide failures. This paper describes the incidence of both mechanisms and discusses the implications for future recommendations for aphid control in the UK.

scholarly journals Inheritance of L1014F and M918T sodium channel mutations associated with pyrethroid resistance in Myzus persicae

2008 ◽  
Vol 4 (5) ◽  
pp. 545-548 ◽  
Author(s):  
Ioannis Eleftherianos ◽  
Stephen P Foster ◽  
Martin S Williamson ◽  
Ian Denholm
Keyword(s):  
Sodium Channel ◽  
Myzus Persicae ◽  
Pyrethroid Resistance ◽  
Pest Species ◽  
Pyrethroid Insecticides ◽  
Potato Aphid ◽  
Direct Dna Sequencing ◽  
Gene Segregation ◽  
Target Site Resistance ◽  
Peach Potato Aphid

Two amino acid substitutions (L1014F and M918T) in the voltage-gated sodium channel confer target-site resistance to pyrethroid insecticides in the peach potato aphid, Myzus persicae . Pyrethroid-resistant and -susceptible M. persicae clones with various combinations of these mutations were crossed under laboratory conditions, and the genotypes of aphid progeny were analysed by direct DNA sequencing of the IIS4–S6 region of the sodium channel gene. Segregation patterns showed that in aphids heterozygous for both L1014F and M918T, both mutations were present in the same resistance allele. Despite these mutations appearing largely recessive in other pest species, such aphids exhibited strong resistance to pyrethroids in leaf-dip bioassays. These results have important implications for the spread and management of pyrethroid resistance in field populations.

scholarly journals The properties of a carboxylesterase from the peach-potato aphid, Myzus persicae (Sulz.), and its role in conferring insecticide resistance

1977 ◽  
Vol 167 (3) ◽  
pp. 675-683 ◽  
Author(s):  
Alan L. Devonshire
Keyword(s):  
Insecticide Resistance ◽  
Myzus Persicae ◽  
Order Rate Constant ◽  
Preliminary Evidence ◽  
Exchange Chromatography ◽  
Potato Aphid ◽  
First Order ◽  
Different Strains ◽  
Hydrolysis Of ◽  
Peach Potato Aphid

Carboxylesterases from different strains of Myzus persicae were examined to try to understand their contribution to insecticide resistance. Preliminary evidence that they are involved comes from the good correlation between the degree of resistance and the carboxylesterase and paraoxon-degrading activity in aphid homogenates. Furthermore the carboxylesterase associated with resistance could not be separated from the insecticide-degrading enzyme by electrophoresis or ion-exchange chromatography. Homogenates of resistant aphids hydrolysed paraoxon 60 times faster than did those of susceptible aphids, yet the purified enzymes from both sources had identical catalytic-centre activities towards this substrate and also towards naphth-1-yl acetate, the latter being hydrolysed by both 2×106 times faster than paraoxon. These observations provide evidence that the enzyme from both sources is identical, and that one enzyme hydrolyses both substrates. This was confirmed by relating the rate of paraoxon hydrolysis to the rate at which paraoxon-inhibited carboxylesterase re-activated. Both had the same first-order rate constant (0.01min−1), showing clearly that the hydrolysis of both substrates is brought about by the same enzyme. Its Km for naphth-1-yl acetate was 0.131mm, and for paraoxon 75pm. The latter very small value could not be measured directly, but was calculated from substrate-competition studies coupled with measurements of re-activation of the diethyl phosphorylated enzyme. Since the purified enzymes from resistant and susceptible aphids had the same catalytic-centre activity, the 60-fold difference between strains must be caused by different amounts of the same enzyme resulting from mutations of the regulator gene(s) rather than of the structural gene.

scholarly journals Establishing the resistance of Myzus persicae (Sulzer) by molecular methods

2008 ◽  
Vol 60 (3) ◽  
pp. 493-499 ◽  
Author(s):  
Andja Vucetic ◽  
Olivera Petrovic-Obradovic ◽  
J. Margaritopoulos ◽  
P. Skouras
Keyword(s):  
Myzus Persicae ◽  
Molecular Methods ◽  
Single Type ◽  
Heterozygous State ◽  
Potato Aphid ◽  
Serbia And Montenegro ◽  
Knock Down ◽  
Peach Potato Aphid

In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investiga?tions, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance.

Transgenic tobacco expressing an Arisaema heterophyllum agglutinin gene displays enhanced resistance to aphids

2004 ◽  
Vol 84 (3) ◽  
pp. 785-790 ◽  
Author(s):  
Jianhong Yao, Xiuyun Zhao ◽  
Huaxiong Qi, Bingliang Wan ◽  
Fei Chen, Xiaofen Sun ◽  
Shanqian Yu ◽  
Kexuan Tang
Keyword(s):  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Myzus Persicae ◽  
Transgenic Tobacco Plants ◽  
Insect Bioassay ◽  
Tobacco Plants ◽  
Potato Aphid ◽  
Enhanced Resistance ◽  
Multiple Copies ◽  
Peach Potato Aphid

Tobacco leaf discs were transformed with a plasmid, pBIAHA, containing the selectable marker neomycin phosphotransferase gene (nptII) and an Arisaema heterophyllum agglutinin gene (aha) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that multiple copies of the aha gene had integrated into the plant genome. Northern blot analysis revealed that the aha gene was expressed at various levels in the transgenic plants. Insect bioassay test showed that transgenic plants expressing multiple copies of the aha gene reduced the rate of population increase of the peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic tobacco plants expressing the aha gene display enhanced resistance to aphids. Key words: Insect bioassay, Arisaema heterophyllum agglutinin, transformation, transgenic tobacco, peach potato aphid (Myzus persicae Sulzer)

New insight into the behaviour modifying activity of two natural sesquiterpenoids farnesol and nerolidol towards Myzus persicae (Sulzer) (Homoptera: Aphididae)

2019 ◽  
Vol 110 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Anna Wróblewska-Kurdyk ◽  
Katarzyna Dancewicz ◽  
Anna Gliszczyńska ◽  
Beata Gabryś
Keyword(s):  
Myzus Persicae ◽  
Total Duration ◽  
Host Plant Selection ◽  
Electrical Penetration Graph ◽  
Potato Aphid ◽  
Epg Technique ◽  
The Mean ◽  
Freely Moving ◽  
Peach Potato Aphid ◽  
Insight Into

AbstractThe effect of structurally related sesquiterpenoids (E,E)-farnesol and cis-nerolidol on the host-plant selection behaviour of the peach potato aphid Myzus persicae (Sulz.) was evaluated using electrical penetration graph (EPG) technique. No repellent effects of (E,E)-farnesol and (Z)-nerolidol to M. persicae were found but aphid probing activities on (E,E)-farnesol- and cis-nerolidol-treated plants were restrained. During non-phloem phases of probing, neither (E,E)-farnesol nor (Z)-nerolidol affected the cell puncture activity. On (E,E)-farnesol-treated plants, the total duration of phloem phase, the mean duration of individual sustained ingestion periods were significantly lower, and the proportion of phloem salivation was higher than on control plants. On (Z)-nerolidol-treated plants, the occurrence of the first phloem phase was delayed, and the frequency of the phloem phase was lower than on control plants. The freely moving aphids were reluctant to remain on (E,E)-farnesol- and (Z)-nerolidol-treated leaves for at least 24 h after exposure. (E,E)-Farnesol and (Z)-nerolidol show complementary deterrent properties, (E,E)-farnesol showing ingestive and post-ingestive activities and nerolidol showing pre-ingestive, ingestive, and post-ingestive deterrent activities.

Sign in / Sign up

Export Citation Format

Share Document