Enhanced metabolism and target gene overexpression confer resistance against acetolactate synthase‐inhibiting herbicides in Bromus sterilis

2021 ◽  
Author(s):  
Madhab Kumar Sen ◽  
Katerina Hamouzová ◽  
Jakub Mikulka ◽  
Rohit Bharati ◽  
Pavlina Košnarová ◽  
...  
Keyword(s):  
Target Gene ◽  
Acetolactate Synthase ◽  
Gene Overexpression

scholarly journals A burden-free gene overexpression system

2021 ◽  
Author(s):  
Carlos Barajas ◽  
Jesse Gibson ◽  
Luis Sandoval ◽  
Domitilla Del Vecchio
Keyword(s):  
Growth Rate ◽  
Target Gene ◽  
Cellular Growth ◽  
Genetic Circuit ◽  
Gene Overexpression ◽  
Genetic Circuits ◽  
Biomolecular Systems ◽  
Synthetic Genes ◽  
Feedforward Controller ◽  
Overexpression System

AbstractOverexpression of synthetic genes depletes cellular resources, particularly ribosomes, which leads to lower expression of other synthetic genes and decreased growth rate. These burden effects can be detrimental to genetic circuit performance and hinders the process of modularly composing genetic circuits to create complex biomolecular systems with novel functions. No solution exists that allows the expression of any gene to a desired level without hindering the expression level of all other genes and growth rate. Here, we engineer an actuator that upregulates ribosome production. The key component of the actuator is a genetic cassette that expresses the hydrolysis domain of the SpoT enzyme (SpoTH) in a cell strain with elevated basal levels of ppGpp. We demonstrate that our actuator is capable of increasing protein production rates (proxy for free ribosomes) by over 150% and growth rate by over 80%. We use the actuator to engineer a feedforward controller, in which SpoTH is co-expressed with a target gene. Expressing the target gene without SpoTH purges the expression of a constitutive gene by more than 80% and cellular growth rate by 40%. By contrast, with SPOTH, the feedforward controller can be tuned to guarantee less than 10% change in the expression of a constitutive gene while keeping the expression of a the target gene at any desired level without any decrease in growth rate (however growth can increase by ≈40%). Alternatively, the feedforward controller can be tuned to guarantee less than 10% deviations in growth rate while also providing 30% higher expression of a constitutive gene relative to the case of expressing the target gene without SpoTH. Therefore, this solution allows desired target gene overexpression without burden, which is instrumental for predictable composition of genetic circuits.

scholarly journals Wilms Tumor–Associated ENL Mutants Cause Target-Gene Overexpression

2020 ◽  
Vol 10 (2) ◽  
pp. 174.2-174
Keyword(s):  
Target Gene ◽  
Wilms Tumor ◽  
Gene Overexpression

Muscle RAS oncogene homolog is a novel therapeutic target gene contributing to hepatocellular carcinoma progression and sorafenib-resistance

2018 ◽  
Vol 56 (01) ◽  
pp. E2-E89
Author(s):  
M Bildstein ◽  
C Hellerbrand ◽  
A Bosserhoff ◽  
P Dietrich
Keyword(s):  
Hepatocellular Carcinoma ◽  
Therapeutic Target ◽  
Target Gene ◽  
Ras Oncogene ◽  
Novel Therapeutic

Investigating miR-125b target gene Arid3a in trisomy 21-associated leukemogenesis

2020 ◽  
Author(s):  
K Weigert ◽  
O Alejo-Valle ◽  
M Labuhn ◽  
V Amstislavskiy ◽  
S Emmrich ◽  
...  
Keyword(s):  
Trisomy 21 ◽  
Target Gene

Identification of Id1 as target gene of profibrogenic TGF-β family signaling during transdifferentiation of hepatic stellate cells

2004 ◽  
Vol 42 (01) ◽  
Author(s):  
E Wiercinska ◽  
L Wickert ◽  
J Hamzavi ◽  
AM Gressner ◽  
S Dooley
Keyword(s):  
Hepatic Stellate Cells ◽  
Target Gene ◽  
Stellate Cells

Suppression of 11[beta]-hydroxysteroid dehydrogenase type 1 target gene regulation by hypoxia

2016 ◽  
Author(s):  
Bushra Shammout ◽  
Adewonuola Alase ◽  
Miriam Wittmann ◽  
Paul Stewart ◽  
Ana Tiganescu
Keyword(s):  
Gene Regulation ◽  
Target Gene ◽  
Hydroxysteroid Dehydrogenase

Differential Acetolactate Synthase (ALS) Inhibitor Sensitivity in Three Biotypes of Cirsium arvense (L.) Scop. in Eastern Europe

2005 ◽  
Vol 40 (1-2) ◽  
pp. 67-78
Author(s):  
P. Nagy ◽  
A. R. Thompson ◽  
M. Schultz ◽  
P. Solymosi
Keyword(s):  
Eastern Europe ◽  
Acetolactate Synthase ◽  
Cirsium Arvense ◽  
Als Inhibitor

2049-P: The Chd4 Helicase of the Nucleosome Remodeling and Deacetylase Complex Modulates Pdx1 Target Gene Expression In Vitro

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2049-P
Author(s):  
REBECCA K. DAVIDSON ◽  
NOLAN CASEY ◽  
JASON SPAETH
Keyword(s):  
Gene Expression ◽  
Target Gene ◽  
Nucleosome Remodeling ◽  
Target Gene Expression
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