Chemical synthesis of a two-domain scorpion toxin LaIT2 and its single-domain analogs to elucidate structural factors important for insecticidal and antimicrobial activities

2018 ◽  
Vol 24 (12) ◽  
pp. e3133 ◽  
Author(s):  
Hironori Juichi ◽  
Ryo Ando ◽  
Takafumi Ishido ◽  
Masahiro Miyashita ◽  
Yoshiaki Nakagawa ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Antimicrobial Activities ◽  
Single Domain ◽  
Scorpion Toxin ◽  
Structural Factors

scholarly journals Chemical Synthesis and Characterization of Maurotoxin, a Short Scorpion Toxin with four Disulfide Bridges that Acts on K+ Channels

1996 ◽  
Vol 242 (3) ◽  
pp. 491-498 ◽  
Author(s):  
Ryadh Kharrat ◽  
Kamel Mabrouk ◽  
Marcel Crest ◽  
Herve Darbon ◽  
Razika Oughideni ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Synthesis And Characterization ◽  
Disulfide Bridges ◽  
K Channels

scholarly journals Isolation, Characterization and Chemical Synthesis of Large Spectrum Antimicrobial Cyclic Dipeptide (l-leu-l-pro) from Streptomyces misionensis V16R3Y1 Bacteria Extracts. A Novel 1H NMR Metabolomic Approach

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 270
Author(s):  
Ilhem Saadouli ◽  
Imène Zendah El Euch ◽  
Emna Trabelsi ◽  
Amor Mosbah ◽  
Alaeddine Redissi ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Chemical Synthesis ◽  
1H Nmr ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Penicillium Expansum ◽  
Cyclic Dipeptide ◽  
Synthetic Compound ◽  
Streptomyces Species

Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensis V16R3Y1 isolated from the date palm rhizosphere (southern Tunisia). This strain presented a broad range of antifungal activity against Fusarium oxysporum, Aspergillus flavus, Penicillium expansum, Aspergillus niger, Candida albicans, Candida metapsilosis, and Candida parapsilosis and antibacterial activity against human pathogenic bacteria, including Escherichia fergusonii, Staphylococcus aureus, Salmonella enterica, Enterococcus faecalis, Bacillus cereus and Pseudomonas aeruginosa. The purification procedure entailed ethyl acetate extract, silica gel column, and thin layer chromatography. Based on 1H NMR metabolomic procedure application, also supported by the GC-MS analysis, cyclic dipeptide (l-Leucyl-l-Proline) was identified as the major compound in the bioactive fraction. In order to confirm the identity of the active compound and to have a large quantity thereof, a chemical synthesis of the cyclic dipeptide was performed. The synthetic compound was obtained with a very good yield (50%) and presented almost the same effect compared to the extracted fraction. This study indicates for the first time that Streptomyces misionensis V16R3Y1 exhibits a broad spectrum of antimicrobial activities, produced cyclic dipeptide (l-Leucyl-l-Proline) and might have potential use as a natural agent for pharmaceutical and agri-food applications.

Comparison of different strategies towards the chemical synthesis of long‐chain scorpion toxin AaH‐II

2021 ◽  
Author(s):  
Yu‐Lei Li ◽  
Qian Qu ◽  
Yun‐Kun Qi ◽  
Lei Liu ◽  
Ke Wei Wang ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Long Chain

Structure revision of the scorpion toxin noxiustoxin through total chemical synthesis

Peptides 1992 ◽  
1993 ◽  
pp. 101-102
Author(s):  
Ruth F. Nutt ◽  
Byron H. Arison ◽  
Jeffrey S. Smith
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Structure Revision

The first chemical synthesis of a short scorpion toxin cross-linked by four disulfide bridges, maurotoxin

Toxicon ◽  
1997 ◽  
Vol 35 (12) ◽  
pp. 1666 ◽  
Author(s):  
R. Kharrat ◽  
K. Mabrouk ◽  
M. Crest ◽  
H. Darbon ◽  
R. Oughideni ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Disulfide Bridges

scholarly journals Chemical synthesis and characterization of Pi1, a scorpion toxin from Pandinus imperator active on K+ channels

2000 ◽  
Vol 267 (16) ◽  
pp. 5149-5155 ◽  
Author(s):  
Ziad Fajloun ◽  
Edmond Carlier ◽  
Catherine Lecomte ◽  
Sandrine Geib ◽  
Eric di Luccio ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Synthesis And Characterization ◽  
K Channels

Chemical synthesis and characterization of maurocalcine, a scorpion toxin that activates Ca2+release channel/ryanodine receptors

FEBS Letters ◽  
2000 ◽  
Vol 469 (2-3) ◽  
pp. 179-185 ◽  
Author(s):  
Z. Fajloun ◽  
R. Kharrat ◽  
L. Chen ◽  
C. Lecomte ◽  
E. Di Luccio ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Ryanodine Receptors ◽  
Scorpion Toxin ◽  
Synthesis And Characterization ◽  
Release Channel

Chemical synthesis and structure-activity relationships of Ts κ, a novel scorpion toxin acting on apamin-sensitive SK channel

1999 ◽  
Vol 54 (5) ◽  
pp. 369-376 ◽  
Author(s):  
C. Lecomte ◽  
G. Ferrat ◽  
Z. Fajloun ◽  
J. Van Rietschoten ◽  
H. Rochat ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Scorpion Toxin ◽  
Structure Activity Relationships ◽  
Sk Channel ◽  
Structure Activity

Polarity Determination in Sphalerite and Wurtzite Structures

1990 ◽  
Vol 48 (2) ◽  
pp. 500-501
Author(s):  
Stuart McKernan ◽  
C. Barry Carter
Keyword(s):  
Opposite Polarity ◽  
Single Domain ◽  
Polar Material ◽  
Electron Microscopic ◽  
Dynamical Interaction ◽  
X Ray ◽  
Polarity Determination ◽  
The Absolute ◽  
Microscopic Techniques

The determination of the absolute polarity of a polar material is often crucial to the understanding of the defects which occur in such materials. Several methods exist by which this determination may be performed. In bulk, single-domain specimens, macroscopic techniques may be used, such as the different etching behavior, using the appropriate etchant, of surfaces with opposite polarity. X-ray measurements under conditions where Friedel’s law (which means that the intensity of reflections from planes of opposite polarity are indistinguishable) breaks down can also be used to determine the absolute polarity of bulk, single-domain specimens. On the microscopic scale, and particularly where antiphase boundaries (APBs), which separate regions of opposite polarity exist, electron microscopic techniques must be employed. Two techniques are commonly practised; the first [1], involves the dynamical interaction of hoLz lines which interfere constructively or destructively with the zero order reflection, depending on the crystal polarity. The crystal polarity can therefore be directly deduced from the relative intensity of these interactions.

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