Photodynamic Therapy of Pheophorbide a Inhibits the Proliferation of Human Breast Tumour via Both Caspase-dependent and -independent Apoptotic Pathways in In Vitro and In Vivo Models

2011 ◽  
Vol 26 (5) ◽  
pp. 734-742 ◽  
Author(s):  
Sandy Wan-Heng Hoi ◽  
Hoi Ming Wong ◽  
Judy Yuet-Wa Chan ◽  
Grace Gar Lee Yue ◽  
Gary Man-Kit Tse ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Breast Tumour ◽  
Human Breast ◽  
Pheophorbide A ◽  
In Vivo Models ◽  
Human Breast Tumour ◽  
Apoptotic Pathways

In Vitro and In Vivo Efficacy of Photofrin® and Pheophorbide a, a Bacteriochlorin, in Photodynamic Therapy of Colonic Cancer Cells¶

2002 ◽  
Vol 75 (2) ◽  
pp. 140 ◽  
Author(s):  
A. Hajri ◽  
S. Wack ◽  
C. Meyer ◽  
M. K. Smith ◽  
C. Leberquier ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Colonic Cancer ◽  
Pheophorbide A ◽  
In Vivo Efficacy

Photodynamic therapy can kill Cryptococcus neoformans in in vitro and in vivo models

2009 ◽  
Author(s):  
Renato A. Prates ◽  
Eriques G. da Silva ◽  
Priscila F. Chaves ◽  
Antônio José S. Santos ◽  
Claudete R. Paula ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cryptococcus Neoformans ◽  
In Vivo Models

Beyond mouse cancer models: Three-dimensional human-relevant in vitro and non-mammalian in vivo models for photodynamic therapy

2017 ◽  
Vol 773 ◽  
pp. 242-262 ◽  
Author(s):  
Malgorzata Kucinska ◽  
Marek Murias ◽  
Patrycja Nowak-Sliwinska
Keyword(s):  
Photodynamic Therapy ◽  
Three Dimensional ◽  
In Vivo Models ◽  
Cancer Models ◽  
Mouse Cancer Models

In Vitro and In Vivo Efficacy of Photofrin® and Pheophorbide a, a Bacteriochlorin, in Photodynamic Therapy of Colonic Cancer Cells¶

2007 ◽  
Vol 75 (2) ◽  
pp. 140-148 ◽  
Author(s):  
A. Hajri ◽  
S. Wack ◽  
C. Meyer ◽  
M. K. Smith ◽  
C. Leberquier ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Colonic Cancer ◽  
Pheophorbide A ◽  
In Vivo Efficacy

Photodynamic therapy for rat pituitary tumor in vitro and in vivo using pheophorbide-a and white light

1991 ◽  
Vol 11 (2) ◽  
pp. 174-182 ◽  
Author(s):  
Takashi Yano ◽  
Tohru Uozumi ◽  
Keiichi Kawamoto ◽  
Kazutoshi Mukada ◽  
Jun Onda ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
White Light ◽  
Pituitary Tumor ◽  
Pheophorbide A ◽  
Rat Pituitary

Altered expression in vitro of human breast tumour associated glycoproteins by hormone additives

1988 ◽  
Vol 16 (6) ◽  
pp. 1026-1027
Author(s):  
PHILIP D. RYE ◽  
ROSEMARY A. WALKER
Keyword(s):  
Breast Tumour ◽  
Human Breast ◽  
Altered Expression ◽  
Human Breast Tumour

scholarly journals Hypericin and Pheophorbide a Mediated Photodynamic Therapy Fighting MRSA Wound Infections: A Translational Study from In Vitro to In Vivo

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1399
Author(s):  
Ben Chung Lap Chan ◽  
Priyanga Dharmaratne ◽  
Baiyan Wang ◽  
Kit Man Lau ◽  
Ching Ching Lee ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Infection Model ◽  
Wound Infections ◽  
Pheophorbide A ◽  
Promising Alternative ◽  
Translational Study ◽  
Therapeutic Modalities ◽  
High Prevalence

High prevalence rates of methicillin-resistant Staphylococcus aureus (MRSA) and lack of effective antibacterial treatments urge discovery of alternative therapeutic modalities. The advent of antibacterial photodynamic therapy (aPDT) is a promising alternative, composing rapid, nonselective cell destruction without generating resistance. We used a panel of clinically relevant MRSA to evaluate hypericin (Hy) and pheophobide a (Pa)-mediated PDT with clinically approved methylene blue (MB). We translated the promising in vitro anti-MRSA activity of selected compounds to a full-thick MRSA wound infection model in mice (in vivo) and the interaction of aPDT innate immune system (cytotoxicity towards neutrophils). Hy-PDT consistently displayed lower minimum bactericidal concentration (MBC) values (0.625–10 µM) against ATCC RN4220/pUL5054 and a whole panel of community-associated (CA)-MRSA compared to Pa or MB. Interestingly, Pa-PDT and Hy-PDT topical application demonstrated encouraging in vivo anti-MRSA activity (>1 log10 CFU reduction). Furthermore, histological analysis showed wound healing via re-epithelization was best in the Hy-PDT group. Importantly, the dark toxicity of Hy was significantly lower (p < 0.05) on neutrophils compared to Pa or MB. Overall, Hy-mediated PDT is a promising alternative to treat MRSA wound infections, and further rigorous mechanistic studies are warranted.

scholarly journals Photodynamic therapy induces autophagy-mediated cell death in human colorectal cancer cells via activation of the ROS/JNK signaling pathway

2020 ◽  
Vol 11 (10) ◽  
Author(s):  
Changfeng Song ◽  
Wen Xu ◽  
Hongkun Wu ◽  
Xiaotong Wang ◽  
Qianyi Gong ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Photodynamic Therapy ◽  
Signaling Pathway ◽  
Tumor Promoter ◽  
Human Colorectal Cancer ◽  
In Vivo Models ◽  
Jnk Signaling ◽  
Jnk Signaling Pathway

Abstract Evidence has shown that m-THPC and verteporfin (VP) are promising sensitizers in photodynamic therapy (PDT). In addition, autophagy can act as a tumor suppressor or a tumor promoter depending on the photosensitizer (PS) and the cancer cell type. However, the role of autophagy in m-THPC- and VP-mediated PDT in in vitro and in vivo models of human colorectal cancer (CRC) has not been reported. In this study, m-THPC-PDT or VP-PDT exhibited significant phototoxicity, inhibited proliferation, and induced the generation of large amounts of reactive oxygen species (ROS) in CRC cells. From immunoblotting, fluorescence image analysis, and transmission electron microscopy, we found extensive autophagic activation induced by ROS in cells. In addition, m-THPC-PDT or VP-PDT treatment significantly induced apoptosis in CRC cells. Interestingly, the inhibition of m-THPC-PDT-induced autophagy by knockdown of ATG5 or ATG7 substantially inhibited the apoptosis of CRC cells. Moreover, m-THPC-PDT treatment inhibited tumorigenesis of subcutaneous HCT116 xenografts. Meanwhile, antioxidant treatment markedly inhibited autophagy and apoptosis induced by PDT in CRC cells by inactivating JNK signaling. In conclusion, inhibition of autophagy can remarkably alleviate PDT-mediated anticancer efficiency in CRC cells via inactivation of the ROS/JNK signaling pathway. Our study provides evidence for the therapeutic application of m-THPC and VP in CRC.

scholarly journals Identification of Cyanobacterial Strains with Potential for the Treatment of Obesity-Related Co-Morbidities by Bioactivity, Toxicity Evaluation and Metabolite Profiling

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 280 ◽  
Author(s):  
Margarida Costa ◽  
Filipa Rosa ◽  
Tiago Ribeiro ◽  
Rene Hernandez-Bautista ◽  
Marco Bonaldo ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolite Profiling ◽  
Metabolic Regulation ◽  
Complex Disease ◽  
Metabolic Diseases ◽  
Small Animal ◽  
Pheophorbide A ◽  
In Vivo Models

Obesity is a complex disease resulting in several metabolic co-morbidities and is increasing at epidemic rates. The marine environment is an interesting resource of novel compounds and in particular cyanobacteria are well known for their capacity to produce novel secondary metabolites. In this work, we explored the potential of cyanobacteria for the production of compounds with relevant activities towards metabolic diseases using a blend of target-based, phenotypic and zebrafish assays as whole small animal models. A total of 46 cyanobacterial strains were grown and biomass fractionated, yielding in total 263 fractions. Bioactivities related to metabolic function were tested in different in vitro and in vivo models. Studying adipogenic and thermogenic gene expression in brown adipocytes, lipid metabolism and glucose uptake in hepatocytes, as well as lipid metabolism in zebrafish larvae, we identified 66 (25%) active fractions. This together with metabolite profiling and the evaluation of toxicity allowed the identification of 18 (7%) fractions with promising bioactivity towards different aspects of metabolic disease. Among those, we identified several known compounds, such as eryloside T, leptosin F, pheophorbide A, phaeophytin A, chlorophyll A, present as minor peaks. Those compounds were previously not described to have bioactivities in metabolic regulation, and both known or unknown compounds could be responsible for such effects. In summary, we find that cyanobacteria hold a huge repertoire of molecules with specific bioactivities towards metabolic diseases, which needs to be explored in the future.

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