Graphene and graphene oxide: two ideal choices for the enrichment and ionization of long-chain fatty acids free from matrix-assisted laser desorption/ionization matrix interference

2011 ◽  
Vol 25 (21) ◽  
pp. 3223-3234 ◽  
Author(s):  
Yang Liu ◽  
Junyan Liu ◽  
Chunhui Deng ◽  
Xiangmin Zhang
Keyword(s):  
Fatty Acids ◽  
Graphene Oxide ◽  
Laser Desorption ◽  
Long Chain Fatty Acids ◽  
Laser Desorption Ionization ◽  
Matrix Interference ◽  
Desorption Ionization ◽  
Long Chain ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

2020 ◽  
Vol 3 (2) ◽  
pp. 36
Author(s):  
Aleksey Gladchuk ◽  
Julia Shumilina ◽  
Alena Kusnetsova ◽  
Ksenia Bureiko ◽  
Susan Billig ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Sample Preparation ◽  
High Throughput ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative content of free fatty acids (FFAs). In this context, nitrogen-fixing bacteria (order Rhizobiales), the symbionts of legumes, are particularly interesting. Indeed, the FA profiles influence the structure of rhizobial nodulation factors, required for successful infection of plant root. Although FA patterns can be assessed by gas chromatography—(GC-) and liquid chromatography—mass spectrometry (LC-MS), sample preparation for these methods is time-consuming and quantification suffers from compromised sensitivity, low stability of derivatives and artifacts. In contrast, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) represents an excellent platform for high-efficient metabolite fingerprinting, also applicable to FFAs. Therefore, here we propose a simple and straightforward protocol for high-throughput relative quantification of FFAs in rhizobia by combination of Langmuir technology and MALDI-TOF-MS featuring a high sensitivity, accuracy and precision of quantification. We describe a step-by-step procedure comprising rhizobia culturing, pre-cleaning, extraction, sample preparation, mass spectrometric analysis, data processing and post-processing. As a case study, a comparison of the FFA metabolomes of two rhizobia species—Rhizobium leguminosarum and Sinorhizobium meliloti, demonstrates the analytical potential of the protocol.

Sign in / Sign up

Export Citation Format

Share Document